硼替佐米对淋巴瘤细胞增殖凋亡和SHP-2基因表达的影响研究
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摘要
目的观察硼替佐米对淋巴瘤细胞株Jurkat细胞、Raji细胞增殖凋亡和SHP-2基因表达的影响。方法利用淋巴瘤细胞株Jurkat细胞和Raji细胞进行传代培养,用1.0、5.0、25.0和100.0 nmol/L不同浓度的硼替佐米进行处理,观察不同时间段细胞增殖抑制率和凋亡率,比较实验组和对照组SHP-2基因表达水平。结果随着硼替佐米浓度增加,相同干预时间下的Jurkat细胞、Raji细胞抑制率增加;随着干预时间延长,相同硼替佐米浓度时,Jurkat细胞、Raji细胞抑制率也增加。随着硼替佐米浓度增加,相同干预时间下的Jurkat细胞、Raji细胞凋亡率增加;随着干预时间延长,干预浓度相同时,Jurkat细胞、Raji细胞凋亡率也增加。对照组的SHP-2表达量小于硼替佐米干预组,且干预时间相同时,随着浓度增加,SHP-2表达量升高;药物浓度相同,随着干预时间延长,SHP-2表达量升高。结论硼替佐米可抑制淋巴瘤细胞株Jurkat细胞、Raji细胞增殖,促进凋亡并提高SHP-2基因表达量,相关作用与浓度有关。
Objective To observe the effects of bortezomib on apoptosis and expression of SHP-2 gene in the proliferation of lymphoma cell line Jurkat cells and Raji cells.Methods Jurkat cells and Raji cells were used for subculture,and the proliferation inhibition rate and apoptosis rate of 1.0 nmol/L,5.0 nmol/L,25.0 nmol/L and 100.0 nmol/L were observed. The expression level of SHP-2 gene in the experimental group and the control group was compared. Results With the increase of bortezomib concentration,the inhibition rate of Jurkat cells and Raji cells increased within the same intervention time. With the increase of intervention time,the inhibition rate of Jurkat cells and Raji cells also increased at the same bortezomizomi concentration. The expression of SHP-2 in the blank control group was lower than that of the bortezomizomi intervention group. Within the same intervention time,the expression of SHP-2 increased not only with theconcentration,but also with time at the same concentration of drugs.Conclusion Bortezomib significantly affects proliferation,apoptosis and SHP-2 gene expression of lymphoma cell line Jurkat cells and Raji cells in a concentration-dependent manner.
Objective To observe the effects of bortezomib on apoptosis and expression of SHP-2 gene in the proliferation of lymphoma cell line Jurkat cells and Raji cells.Methods Jurkat cells and Raji cells were used for subculture,and the proliferation inhibition rate and apoptosis rate of 1.0 nmol/L,5.0 nmol/L,25.0 nmol/L and 100.0 nmol/L were observed. The expression level of SHP-2 gene in the experimental group and the control group was compared. Results With the increase of bortezomib concentration,the inhibition rate of Jurkat cells and Raji cells increased within the same intervention time. With the increase of intervention time,the inhibition rate of Jurkat cells and Raji cells also increased at the same bortezomizomi concentration. The expression of SHP-2 in the blank control group was lower than that of the bortezomizomi intervention group. Within the same intervention time,the expression of SHP-2 increased not only with theconcentration,but also with time at the same concentration of drugs.Conclusion Bortezomib significantly affects proliferation,apoptosis and SHP-2 gene expression of lymphoma cell line Jurkat cells and Raji cells in a concentration-dependent manner.
引文
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[10]陈曦,霍春波,陈广山,等.消银解毒方颗粒对Jurkat T细胞内JAK1/STAT3信号通路作用机制的研究[J].北京中医药大学学报,2017,40(2):119.
[2]邵霞,蔡佳翌,许壁榆,等.硼替佐米联合有氧氧化抑制剂寡霉素靶向Burkitt淋巴瘤细胞Raji的杀伤作用及机制[J].肿瘤,2016,36(2):127.
[3]陈建忠,贾秀芹.microRNA-214的下调表达对不同种类淋巴瘤细胞耐药性的影响[J].中国临床药理学杂志,2017,33(10):905.
[4]薛曼,杨云惠,索占伟,等.蛋白酪氨酸磷酸酶SHP-2在Ⅰ型代谢型谷氨酸受体诱发痛觉超敏中的作用[J].中国药理学通报,2017,33(2):171.
[5]韩世炜,黄轶群,郑瑞玑.shRNA沉默LSD1基因对Jurkat细胞增殖的影响及机制研究[J].中华血液学杂志,2016,29(1):56.
[6]高美华,李华侨,李冰,等.下调CD59对急性T系白血病细胞株Jurkat凋亡相关分子的影响[J].中国免疫学杂志,2017,33(5):693.
[7]方鹏,张诺芳.SHIP1调控STAT3信号通路对白血病Jurkat细胞活力和凋亡的影响[J].中国病理生理杂志,2018,12(1):87.
[8]张素琴,李建华,王春美,等.微小RNA-195对急性淋巴细胞白血病Jurkat细胞周期和凋亡的影响[J].中华实验外科杂志,2017,34(1):109.
[9]李华侨,高美华,李冰,等.RNAi沉默CD59对急性T系白血病Jurkat细胞株增殖的影响[J].免疫学杂志,2017,19(1):7.
[10]陈曦,霍春波,陈广山,等.消银解毒方颗粒对Jurkat T细胞内JAK1/STAT3信号通路作用机制的研究[J].北京中医药大学学报,2017,40(2):119.