斑叶香妃草的组培快繁
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摘要
目的:以斑叶香妃草为试验材料,探究不同浓度6-苄基氨基嘌呤(6-BA)和萘乙酸(NAA)配比对试管苗增殖的影响,以及不同浓度NAA、吲哚乙酸(IAA)对试管苗生根的影响。方法:选取带顶芽的斑叶香妃草嫩茎进行培养,探究其试管苗腋芽、不定根分化及组培苗生长发育情况。结果:以MS为基本培养基添加浓度配比为0.3mg/L 6-BA+(0.1mg/L NAA)条件下,试管苗增殖倍数为4.06倍;以MS为基本培养基,在NAA为0.09mg/L条件下,试管苗生长趋势最好,叶片嫩绿,生根数最多,根长最长,每株植株平均根数为8.75个,平均根长为2.54cm。结论:MS+(0.3mg/L 6-BA)+(0.1mg/L NAA)对试管苗增殖倍数的影响最为显著,且6-BA与NAA存在交互作用,对外植体的增殖存在极显著差异;MS+0.09mg/L NAA促进试管苗生根的效果最佳,对试管苗生根有极显著差异。
Objective:To investigate the effects of different concentrations of 6-BA and NAA on the multiplication of vitro seedlings,and the effects of different concentrations of NAA and IAA on the rooting of vitro seedlings of Plectranthus coleoides.Methods:The Plectranthus coleoides stem segments with buds were used as the explants for studying the auxillary buds and adventitious roots differentiation and tissue culture seedlings growth.Results:The multiplication rate of vitro seedlings was 4.06 times under the condition of 0.3mg/L 6-BA+0.1mg/L NAA with MS as the basic medium.Vitro seedlings had the best growth trend under the condition of NAA 0.09mg/L with MS as the basic medium,in which vitro seedlings were green with the largest number of roots and the longest roots.The average number of roots per seedling was 8.75,and the average root length was 2.54 cm.Conclusion:MS+0.3mg/L6-BA+0.1mg/L NAA had the most significant effect on the multiplication rate of seedlings in vitro.The 6-BA and NAA were interacted,and had the most significant effect on explants proliferation.MS+NAA 0.09 mg/L had the best effect in inducing the rooting of vitro seedlings,and the significant difference was significant.
Objective:To investigate the effects of different concentrations of 6-BA and NAA on the multiplication of vitro seedlings,and the effects of different concentrations of NAA and IAA on the rooting of vitro seedlings of Plectranthus coleoides.Methods:The Plectranthus coleoides stem segments with buds were used as the explants for studying the auxillary buds and adventitious roots differentiation and tissue culture seedlings growth.Results:The multiplication rate of vitro seedlings was 4.06 times under the condition of 0.3mg/L 6-BA+0.1mg/L NAA with MS as the basic medium.Vitro seedlings had the best growth trend under the condition of NAA 0.09mg/L with MS as the basic medium,in which vitro seedlings were green with the largest number of roots and the longest roots.The average number of roots per seedling was 8.75,and the average root length was 2.54 cm.Conclusion:MS+0.3mg/L6-BA+0.1mg/L NAA had the most significant effect on the multiplication rate of seedlings in vitro.The 6-BA and NAA were interacted,and had the most significant effect on explants proliferation.MS+NAA 0.09 mg/L had the best effect in inducing the rooting of vitro seedlings,and the significant difference was significant.
引文
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[14]张春梅,闫芳,陈杰昌.不同种类、浓度的激素配合使用对银斑百里香种子无菌苗生长的影响[J].北京联合大学学报,2013,27(4):53-57.
[2]吴卓珈,徐哲民,李春涛.芳香杆物的研究进展[J].安徽农业科学,2005,33(12):2393-2396.
[3]王羽梅.中国芳香植物[M].北京:科学出版社,2008.
[4]金钟民,沙伟.香茶菜属植物的研究现状及药用前景[J].齐齐哈尔大学学报,2003,19(4):10-13.
[5]王勤,周至品,李爱媛.香茶菜属植物药理活性研究进展[J].现代医药卫生,2008,24(3):362-364.
[6]盛玉婷.植物组织培养技术及应用进展[J].安徽农学通报,2008,14(9):45-47.
[7]刘晓梦,孟想想,张威威,等.洋甘菊的组织培养技术[J].北方园艺,2018,42(2):72-76.
[8]李敏,张晨光,路喆,等.“大马士革”玫瑰嫩茎组培快繁技术研究[J].江苏农业科学,2016,44(6):81-83.
[9]邵明月,叶子优,陈梦菲,等.羽叶薰衣草的组织培养[J].现代农业科技,2011,40(22):50-51.
[10]邓正正.植物组织培养寻求最佳培养基的方法研究[J].辽宁农业职业技术学院学报,2015,17(2):18-21.
[11]傅华龙,HE T J,吴巧玉.植物生长调节剂的研究与应用[J].生物加工过程,2008,6(4):7-12.
[12]章鹏程,陈瑜,邓衍福,等.6-BA与NAA不同浓度配比对大花蕙兰原球茎诱导的影响[J].杭州师范大学学报(自然科学版),2012,11(4):331-336.
[13]兰伟,杨京霞,王玲,等.黄金香柳的组织培养与快速繁殖[J].阜阳师范学院学报(自然科学版),2013,30(1):36-39.
[14]张春梅,闫芳,陈杰昌.不同种类、浓度的激素配合使用对银斑百里香种子无菌苗生长的影响[J].北京联合大学学报,2013,27(4):53-57.