摘要
目的:探讨朝医补肺元汤对小鼠哮喘模型肺组织中p38蛋白激酶表达的作用及其可能机制。方法:雄性BALB/c小鼠40只随机分成5组。支气管肺泡灌洗液(BALF)中IL-5、IL-13的含量采用酶联免疫吸附法(ELISA)测出,并对支气管肺泡灌洗液中炎症细胞的病理学改变进行观察。应用免疫蛋白质印迹(Western blot)测小鼠肺组织中磷酸化的p38MAPK表达作用强弱。结果:相较于正常对照组,哮喘模型组小鼠BALF中IL-5、IL-13以及肺组织中的磷酸化p38MAPK表达增强(P<0.05)。相比较于小鼠哮喘模型组,朝医补肺元汤低、高剂量组小鼠肺泡灌洗液中的IL-5、IL-13、肺组织磷酸化p38MAPK表达水平显著降低(P<0.05)。综上可证明朝医补肺元汤能显著改善哮喘小鼠肺组织的病理学上的改变。结论:朝医补肺元汤显著治疗哮喘的功效密切相关于阻碍哮喘小鼠p38MAPK信号通路的表达。
Objective: To investigate the effect of traditional Korean medicine Bufeiyuan Decoction on the expression of p38 protein kinase in lung tissue of mice with asthma and its possible mechanism. Methods: Forty BALB/c male mice were randomly divided into 5 groups. The levels of IL-5 and IL-13 in bronchoalveolar lavage fluid(BALF) were measured by enzyme-linked immunosorbent assay(ELISA),and the pathological changes of inflammatory cells in bronchoalveolar lavage fluid were observed.The expression of phosphorylated p38 MAPK in lung tissue was measured by Western blot. Results: Compared with the normal control group,IL-5 and IL-13 in BALF and the expression of phosphorylated p38 MAPK in lung tissue in asthmatic model group were increased(P < 0. 05). Compared with the asthmatic model group,IL-5 and IL-13 in BALF and the expression of phosphorylated p38 MAPK in lung tissue in traditional Korean medicine Bufeiyuan Decoction high and low groups were significantly decreased(P < 0. 05). In summary,it can be proved that the treatment of traditional Korean medicine Bufeiyuan Decoction can significantly improve the pathologic changes of lung tissue in asthmatic mice. Conclusion: The effect of the treatment of asthma is significantly related to the expression of p38 MAPK signaling pathway in asthmatic mice. The treating method of traditional Korean medicine Bufeiyuan Decoction is by blocking the expression of p38 mapk signaling pathway in asthmatic mice.
引文
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