转录组学技术在茶树抗逆性的研究进展
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摘要
茶树(Camellia sinensis)属山茶科山茶属,是一种重要的木本经济作物,其叶片富含多种活性物质,其中尤以儿茶素、咖啡因、茶氨酸及其他游离氨基酸等活性成分对茶叶品质有重要影响。逆境胁迫是影响茶树生长发育、产量及品质的重要因素,人们对茶树应答逆境胁迫的分子机理越来越关注。转录组学作为功能基因组学的重要组成部分,它从RNA水平上全面研究物种的基因表达情况。利用该技术研究逆境胁迫下茶树基因表达的情况,有利于阐明茶树对逆境胁迫的应答机理。本综述主要介绍应用转录组学技术研究茶树应答生物胁迫和非生物胁迫(干旱,温度,盐碱,重金属等)的进展,以期为茶树抗逆性分子机理的研究提供理论参考。
Camellia sinensis belongs to Camelliaceae, Camellia, an important woody cash crop. Its leaves are rich in a variety of active substances, including catechins, caffeine, theanine and other free amino acids. It has an important influence on the quality of tea. Adversity stress is an important factor affecting the growth, yield and quality of tea trees. People pay more and more attention to the molecular mechanism of tea tree response to stress.As an important part of the functional genomics, transcriptomics comprehensively studies the gene expression of species from the RNA level. Using this technology to study the gene expression of tea trees under stress, it is helpful to elucidate the response mechanism of tea trees to stress. This paper mainly introduces the application of transcriptome technology in tea plant respond to a series of abiotic stress involving in drought, temperature, saline,alkaline and heavy metals, etc., and biotic stress so as to provide a reference for the study of molecular mechanism in tea plant under environmental stresses.
Camellia sinensis belongs to Camelliaceae, Camellia, an important woody cash crop. Its leaves are rich in a variety of active substances, including catechins, caffeine, theanine and other free amino acids. It has an important influence on the quality of tea. Adversity stress is an important factor affecting the growth, yield and quality of tea trees. People pay more and more attention to the molecular mechanism of tea tree response to stress.As an important part of the functional genomics, transcriptomics comprehensively studies the gene expression of species from the RNA level. Using this technology to study the gene expression of tea trees under stress, it is helpful to elucidate the response mechanism of tea trees to stress. This paper mainly introduces the application of transcriptome technology in tea plant respond to a series of abiotic stress involving in drought, temperature, saline,alkaline and heavy metals, etc., and biotic stress so as to provide a reference for the study of molecular mechanism in tea plant under environmental stresses.
引文
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Das A.,Das S.,and Mondal T.K.,2012,Identification of differentially expressed gene profiles in young roots of tea(Camellia sinensis(L.)O.Kuntze)subjected to drought stress using suppression subtractive hybridization,Plant Mol.Biol Rep.,30(5):1088-1101
Das A.,Mukhopadhyai M.,Sarkar B.,Saha D.,and Mondal T.K.2015,Influen ce of drought stress on cellular ultrastructure and antioxidant system in tea cultivars with different drought sensitivities,J.Environ.Biol.,36(4):875-882
Deng W.W.,Han J.Y.,Fan Y.B.,Tai Y.L.,Zhu B.Y.,Lu M.Q.,Wang R.J.,Wan X.C.,and Zhang Z.Z.,2018,Uncovering tea-specific secondary metabolism using transcriptomic and metabolomic analyses in grafts of Camellia sinensis and C.oleifera,Tree Genet.Genom.,14(2):23
Gao J.,Luo M.,Zhu Y.,He Y.,Wang Q.,and Zhang C.,2015,Transcriptome sequencing and differential gene expression analysis in Viola yedoensis Makino(Fam.Violaceae)responsive to cadmium(Cd)pollution,Biochem.Biophys.Res.Commun.,459(1):60-65
Glaubitz U.,Li X.,Schaedel S.,Erban A.,Sulpice R.,Kopka J.,Hincha D.K.,and Zuther E.,2017,Integrated analysis of rice transcriptomic and metabolomic responses to elevated night temperatures identifies sensitivity-and tolerance-related profiles,Plant Cell Environ.,40(1):121-137
Gupta S.,Bharalee R.,Bhorali P.,Bandyopadhyay T.,Gohain B.,Agarwal N.,Ahmed P.,Saikia H.,Borchetia S.,Kalita M.C.,Handique A.K.,and Das S.,2012,Identification of drought tolerant progenies in tea by gene expression analysis,Funct.Integr.Genomics,12(3):543-563
Gupta S.,Bharalee R.,Bhorali P.,Das S.K.,Bhagawati P.,Bandyopadhyay T.,Gohain B.,Agarwal N.,Ahmed P.,Borchetia S.,Kalita M.C.,Handique A.K.,and Das S.,2013,Molecular analysis of drought tolerance in tea by cD-NA-AFLP based transcript profiling,Mol.Biotechnol.,53(3):237-248
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Lai J.D.,2016,Gene expression analysis of tea leaves in response to Colletotrichum kahawae infection revealed by cDNA-AF-LP,Thesis for M.S.,Fujian Agriculture and Forestory University,Supervisor:Liu W.,pp.31-44(赖建东,2016,炭疽菌侵染后茶树cDNA-AFLP体系构建和基因差异表达研究,硕士学位论文,福建农林大学,导师:刘伟,pp.31-44)
Li N.N.,Yue C.,Cao H.L.,Qian W.J.,Hao X.Y.,Wang Y.C.,Wang L.,Ding C.Q.,Wang X.C.,and Yang Y.J.,2018,Transcriptome sequencing dissection of the mechanisms underlying differential cold sensitivity in young and mature leaves of the tea plant(Camellia sinensis),J.Plant Physiol.,224-225:144-155
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