福建省HIV-1毒株亚型分布分析
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摘要
目的分析福建省HIV-1毒株亚型毒株的分布特点。方法选取福建医科大学孟超肝胆医院就医的312例HIV-1感染者,采用TaKaLa PrimeScipt~(TM) One Step RT-PCR Ver.2和Prime Ex Taq~(TM) Hot Start Version试剂盒对gag基因区和env基因区进行扩增并测定其序列。结果 312例标本结果显示,131例为CRF07_BC(41.99%),112例为CRF01_AE(35.89%),69例为B亚型(22.12%),性传播是主要感染途径。结论福建省HIV-1毒株亚型分布众多,以CRF07_BC、CRF01_AE流行重组型为主,经性传播为主要感染途径。基于此,需加强对福建省HIV-1感染的监测,有针对性的开展行为干预,尤其是性工作者和同性性传播群体的监控,同时结合福建省HIV-1感染情况开展疫苗研发工作,有效防控区域艾滋病传播。
Objective To analyze the distribution characteristics of HIV-1 subtype in Fujian province. Methods Using TaKaLa PrimeScit~(TM) One Step RT-PCR Ver.2 and Prime ExTaq~(TM) Hot Start Verse reagent kit, 312 cases of HIV-1 infected patients were selected from Mengchao Hepatobiliary Hospital of Fujian Medical University to amplify the gag gene region and env gene region and determine its sequence. Results The results of 312 specimens showed that 131 cases were CRF07_BC(41.99%), 112 cases were CRF01_AE(35.89%), and 69 cases were subtype B(22.12%). Sexual transmission was the main route of infection. Conclusion There are many subtypes of HIV-1 strains in Fujian Province, mainly CRF07_BC and CRF01_AE, and sexually transmitted as the main route of infection. Based on this, it is necessary to strengthen the monitoring of HIV-1 infection in Fujian Province,conduct targeted behavioral interventions, especially the monitoring of sex workers and same-sex sexually transmitted groups, and carry out vaccine research and development in conjunction with HIV-1 infection in Fujian Province to effectively prevent and control regional HIV transmission.
Objective To analyze the distribution characteristics of HIV-1 subtype in Fujian province. Methods Using TaKaLa PrimeScit~(TM) One Step RT-PCR Ver.2 and Prime ExTaq~(TM) Hot Start Verse reagent kit, 312 cases of HIV-1 infected patients were selected from Mengchao Hepatobiliary Hospital of Fujian Medical University to amplify the gag gene region and env gene region and determine its sequence. Results The results of 312 specimens showed that 131 cases were CRF07_BC(41.99%), 112 cases were CRF01_AE(35.89%), and 69 cases were subtype B(22.12%). Sexual transmission was the main route of infection. Conclusion There are many subtypes of HIV-1 strains in Fujian Province, mainly CRF07_BC and CRF01_AE, and sexually transmitted as the main route of infection. Based on this, it is necessary to strengthen the monitoring of HIV-1 infection in Fujian Province,conduct targeted behavioral interventions, especially the monitoring of sex workers and same-sex sexually transmitted groups, and carry out vaccine research and development in conjunction with HIV-1 infection in Fujian Province to effectively prevent and control regional HIV transmission.
引文
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[2]高景波. HIV酶联免疫吸附试验检测设置灰区的必要性[J].中国艾滋病性病,2016,22(12):1009-1010.
[3]刘佳,闫江舟,孙定勇,等. 2015年河南省新确证HIV-1感染者耐药传播状况研究[J].河南医学研究,2018,27(4):598-601.
[4]佐合拉·吐尔地,廖玲洁,孙磊,等.新疆乌鲁木齐市2014年HIV-1耐药毒株传播分析[J].中国艾滋病性病,2016,22(8):586-588.
[5]吴楠楠,殷玥琪,袁瑞,等.我国HIV亚型与耐药性Meta分析[J].中华流行病学杂志, 2016,37(11):1546-1554.
[6]赵帅,冯毅,辛若雷,等.应用分子传播网络研究北京男男性行为者HIV-1毒株的传播特征[J].中国艾滋病性病,2018,24(3):241-245,306.
[7]韩志刚,吴昊,梁彩云,等.广州市2008-2010年和2015年外籍HIV-1感染者病毒亚型分析[J].中华流行病学杂志,2017,38(6):805-809.
[8]吴可可,朱紫苗,苏德华,等.温州地区HIV/AIDS病人抗病毒治疗后耐药基因变异研究[J].中国艾滋病性病,2018,24(1):40-43.
[9]McCoy LE,Burton DR. Identification and specificity of broadly neutralizing antibodies against HIV[J]. Immunol Rev,2017,275(1):11-20.
[10]王毅,李六林,樊静,等.男男性行为人群应对方式现状及与人口学特征的关系[J].中国病毒病杂志,2016,6(5):351-355.
[11]Pines HA,Wertheim JO,Liu L,et al. Concurrency and HIV transmission network characteristics among men who have sex with men with recent HIV infection[J]. AIDS,2016,28(30):2875-2883.
[12]邱月锋,张春阳,夏品苍,等.福建省2013年艾滋病病毒耐药警戒线调查[J].中国病毒病杂志,2016,6(4):275-278.
[13]袁丹,叶黎,龚芳红,等. 2014年四川省部分地区HIV/AIDS患者抗病毒治疗效果和耐药性影响因素分析[J].现代预防医学,2016,43(13):2445-2452.