基于射干苷临床利用量的川射干质量评价研究
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摘要
以射干苷为指标,采用单因素实验法考察浸泡对川射干提取转移率的影响,采用正交试验法优选川射干的最佳提取工艺,采用HPLC法测定10批川射干中射干苷含量,计算其临床利用量.结果显示:川射干的最佳提取工艺为,浸泡至过心后加8倍量水,提取2次,每次2 h; 10批川射干中射干苷含量与临床利用量呈负相关.结果表明,建立的评价方法将药材中化学成分与实际利用效果相结合,为川射干及其他中药材品质评价提供了一种新思路.
The effect of soaking on the Iridis Tectori Rhizoma's extract transport rate is tested by the single factor experiment based on the index of tectoridin,and the optimum extraction process is selected by the orthogonal test. The content of the tectoridin in 10 batches of Iridis Tectori Rhizomais is determined by HPLC method,and the clinical utilization is calculated. The best extraction process for Iridis Tectori Rhizomais is to add 8 times of water after it has been soaked with the water getting through the center,and extract it 2 times for 2 hours each time. The content of tectoridin in the 10 batches of Iridis Tectori Rhizoma is negatively correlated with its clinical utilization. It is concluded that the method established in this study combines the chemical components in the medicinal materials with the actual utilization effects,and provides a new idea for the quality evaluation of Iridis Tectori Rhizoma and of other Chinese herbal medicines.
The effect of soaking on the Iridis Tectori Rhizoma's extract transport rate is tested by the single factor experiment based on the index of tectoridin,and the optimum extraction process is selected by the orthogonal test. The content of the tectoridin in 10 batches of Iridis Tectori Rhizomais is determined by HPLC method,and the clinical utilization is calculated. The best extraction process for Iridis Tectori Rhizomais is to add 8 times of water after it has been soaked with the water getting through the center,and extract it 2 times for 2 hours each time. The content of tectoridin in the 10 batches of Iridis Tectori Rhizoma is negatively correlated with its clinical utilization. It is concluded that the method established in this study combines the chemical components in the medicinal materials with the actual utilization effects,and provides a new idea for the quality evaluation of Iridis Tectori Rhizoma and of other Chinese herbal medicines.
引文
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[11]孔馨逸,刘妍如,唐志书,等.响应面法优选葛根超微粉的制备工艺及活性成分测定研究[J].中草药,2018,49(13):2993-3000.
[2]潘静.川射干化学成分及体外抗肿瘤活性的研究[D].武汉:湖北中医学院,2009.
[3]韩梅,金光洙,朴惠顺.异黄酮类成分抗肿瘤作用研究进展[J].中草药,2006,37(9):1437-1439.
[4]于颖.川射干异黄酮类化学成分的神经保护作用[D].泰安:泰山医学院,2013.
[5]刘铁秋,曾宪垠,刘杨红,等.川射干抑菌成分提取及对番茄灰霉抑菌作用研究[J].中国抗生素杂志,2014,39(11):813-818.
[6]林启寿.中草药成分化学[M].北京:科学出版社,1977.
[7]刘涛,郭晓恒,吴春梅,等.肺毒清颗粒中有效成分的含量测定及转移率考察[J].中国实验方剂学杂志,2013,19(10):54-56.
[8]刘涛,苟小军,万德光,等.基于“中药成分临床利用率"的中药材药用品质评价模式的商建[J].中草药,2015,46(13):1863-1866.
[9]柏璐.正交试验法优选川射干的提取工艺[J].黑龙江医药,2009,22(3):333-334.
[10]井乐刚,张永忠.大豆异黄酮的物理化学性质[J].中国农学通报,2006,22(1):85-87.
[11]孔馨逸,刘妍如,唐志书,等.响应面法优选葛根超微粉的制备工艺及活性成分测定研究[J].中草药,2018,49(13):2993-3000.