基于紫外—可见与红外指纹图谱的金银花与山银花品质差异研究
|
摘要
采用紫外—可见分光光度计和红外光谱仪分别测定10批金银花药材与10批山银花药材,建立金银花药材的对照紫外—可见及红外指纹图谱,并利用相关系数法计算10批山银花样品紫外—可见指纹图谱与对照指纹图谱的相似度,建立红外指纹图谱共有峰率和变异峰率双指标,分析金银花与山银花质量差异.结果显示,在紫外—可见指纹图谱中,山银花药材图谱与对照图谱的相似度均在0. 957以上,在红外指纹图谱中,金银花样品与山银花变异峰率大于共有峰率.结果表明,以紫外—可见指纹图谱评价金银花和山银花质量时,二者内在质量相似,但以红外指纹图谱评价二者质量时,内在质量差异较大.
Ten batches of Flos Lonicerae and ten batches of Radix Lonicerae were determined by UVVis spectrophotometer and infrared spectrometer,and the UV and IR fingerprints of Flos Lonicerae were established.The correlation coefficient method was used to calculate the similarity between the UV fingerprint and the control fingerprint of ten batches of Lonicera japonica.The common peak rate and variation peak rate of the infrared fingerprint were established.The quality differences between honeysuckle and honeysuckle were analyzed.In the UV fingerprint,the similarity between the UV spectrum and the control spectrum was more than 0.957,and in the infrared fingerprint,the variation peak rate of honeysuckle and Flos Lonicerae was higher than that of common peak.The experimental results showed that when the quality of honeysuckle and Flos Lonicerae was evaluated by UV fingerprint,the intrinsic quality of both was similar,but when the quality of Flos Lonicerae and Honeysuckle was evaluated by infrared fingerprint,the internal quality varied greatly.
Ten batches of Flos Lonicerae and ten batches of Radix Lonicerae were determined by UVVis spectrophotometer and infrared spectrometer,and the UV and IR fingerprints of Flos Lonicerae were established.The correlation coefficient method was used to calculate the similarity between the UV fingerprint and the control fingerprint of ten batches of Lonicera japonica.The common peak rate and variation peak rate of the infrared fingerprint were established.The quality differences between honeysuckle and honeysuckle were analyzed.In the UV fingerprint,the similarity between the UV spectrum and the control spectrum was more than 0.957,and in the infrared fingerprint,the variation peak rate of honeysuckle and Flos Lonicerae was higher than that of common peak.The experimental results showed that when the quality of honeysuckle and Flos Lonicerae was evaluated by UV fingerprint,the intrinsic quality of both was similar,but when the quality of Flos Lonicerae and Honeysuckle was evaluated by infrared fingerprint,the internal quality varied greatly.
引文
[1]国家药典委员会.中华人民共和国药典[S].北京:中国医药科技出版社,2015.
[2]康帅,张继,王亚丹,等.金银花与山银花的生药学鉴别研究[J].药物分析杂志,2014,34(11):1913-1921.
[3]刘玉琴.山银花中皂苷含量测定及指纹图谱研究[D].长沙:湖南中医药大学,2014.
[4]吴飞燕,冯宋岗,曾建国.金银花与山银花的鉴别与归属研究[J].中草药,2014,45(8):1150-1156.
[5]郑义,陈晓兰,丁宁,等.金银花与山银花有效成分测定及比较分析[J].甘肃农业大学学报,2015,50(4):170-174.
[6]刘亚楠,石典花,雷留成.金银花与山银花的HPLC指纹图谱鉴别研究[J].中草药,2017,48(4):773-776.
[7]王栋,王永禄,郭啸,等.中药金礞石红外指纹图谱相似度分析[J].光谱学与光谱分析,2011,31(10):2715-2718.
[8]田润涛,谢培山.色谱指纹图谱相似度评价方法的规范化研究(一)[J].中药新药与临床药理,2006,17(1):40-42.
[9]张军锋,杜宁,谭伟航,等.莪术红外指纹图谱研究[J].江苏农业科学,2017,45(10):40-42.
[10]关瑾光.近代傅立叶变换红外光谱技术及应用[M].北京:科技文献出版社,1994.
[2]康帅,张继,王亚丹,等.金银花与山银花的生药学鉴别研究[J].药物分析杂志,2014,34(11):1913-1921.
[3]刘玉琴.山银花中皂苷含量测定及指纹图谱研究[D].长沙:湖南中医药大学,2014.
[4]吴飞燕,冯宋岗,曾建国.金银花与山银花的鉴别与归属研究[J].中草药,2014,45(8):1150-1156.
[5]郑义,陈晓兰,丁宁,等.金银花与山银花有效成分测定及比较分析[J].甘肃农业大学学报,2015,50(4):170-174.
[6]刘亚楠,石典花,雷留成.金银花与山银花的HPLC指纹图谱鉴别研究[J].中草药,2017,48(4):773-776.
[7]王栋,王永禄,郭啸,等.中药金礞石红外指纹图谱相似度分析[J].光谱学与光谱分析,2011,31(10):2715-2718.
[8]田润涛,谢培山.色谱指纹图谱相似度评价方法的规范化研究(一)[J].中药新药与临床药理,2006,17(1):40-42.
[9]张军锋,杜宁,谭伟航,等.莪术红外指纹图谱研究[J].江苏农业科学,2017,45(10):40-42.
[10]关瑾光.近代傅立叶变换红外光谱技术及应用[M].北京:科技文献出版社,1994.