植物槲皮素对东方田鼠免疫功能的影响
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摘要
为探讨植物次生化合物对植食性小哺乳动物免疫功能的影响,本研究以含0、1. 5%、3%、5%和8%槲皮素的食物饲喂东方田鼠,探究东方田鼠的免疫器官发育和细胞因子水平对不同浓度槲皮素的响应。结果显示:不同浓度植物槲皮素对东方田鼠的体重增长均无显著影响;与不含槲皮素(对照组)相比,5%槲皮素处理的东方田鼠个体的脾脏和胸腺指数分别增加14. 48%(P <0. 05)、11. 67%(P <0. 05),细胞因子IL-2、IL-10分别增加8. 90%(P <0. 05)、14. 99%(P <0. 05),而TNF-α、IL-1β分别降低11. 13%(P <0. 05)、17. 40%(P <0. 05);槲皮素浓度达到8%时,与5%相比,脾脏指数、胸腺指数降低,IL-2、IL-10分泌减少,而TNF-α、IL-1β分泌升高。表明不同浓度植物槲皮素对东方田鼠免疫器官指数和细胞因子分泌的影响存在差异。
To explore the relationship between plant secondary compounds and the immune functions of small mammal herbivores,this study measured the effects of plant quercetin on immune organ development and concentrations of several key cytokines of Microtus fortis fed diets containing 0,1. 5%,3%,5%,or 8% quercetin. The results showed that the indices of the spleen and the thymus of M. fortis fed 5% quercetin were increased by 14. 48%( P < 0. 05) and 11. 67%( P <0. 05),respectively,and IL-2 and IL-10 were increased by 8. 90%( P < 0. 05) and 14. 99%( P < 0. 05),respectively; TNF-α and IL-1β were decreased by 11. 13%( P < 0. 05) and 17. 40%( P < 0. 05),respectively,when compared those of the control. When quercetin concentration reached 8%,the indexes of the spleen and the thymus were decreased,IL-2,and IL-10 secretions were suppressed,while TNF-α and IL-1β levels were increased. Dietary plant quercetin affected the immune organ development and cytokine secretion of M. fortis in a dose-dependent manner.
To explore the relationship between plant secondary compounds and the immune functions of small mammal herbivores,this study measured the effects of plant quercetin on immune organ development and concentrations of several key cytokines of Microtus fortis fed diets containing 0,1. 5%,3%,5%,or 8% quercetin. The results showed that the indices of the spleen and the thymus of M. fortis fed 5% quercetin were increased by 14. 48%( P < 0. 05) and 11. 67%( P <0. 05),respectively,and IL-2 and IL-10 were increased by 8. 90%( P < 0. 05) and 14. 99%( P < 0. 05),respectively; TNF-α and IL-1β were decreased by 11. 13%( P < 0. 05) and 17. 40%( P < 0. 05),respectively,when compared those of the control. When quercetin concentration reached 8%,the indexes of the spleen and the thymus were decreased,IL-2,and IL-10 secretions were suppressed,while TNF-α and IL-1β levels were increased. Dietary plant quercetin affected the immune organ development and cytokine secretion of M. fortis in a dose-dependent manner.
引文
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Angelika B,Jutta W,Martin A,Nicolas R,Humpert P M,Dimitri P,Roman F,Maximilian V E,Thoralf W,Gottfried R,Martina J,Michael M,Markus S,Bruce M,Clemens K,Peter P N.2003.Amechanism converting psychosocial stress into mononuclear cell activation.Proc Natl Acad Sci USA,100(4):1920-1925.
Bergeron J M,Jodoin L,Jean Y.1987.Pathology voles(Microtus pennsylvanicus)fed with plant extracts.Journal of Mammalogy,68(1):73-79.
Boesch S C,Loboda A,Wagner A E,Stachurska A,Jozkowicz A,Dulak J,D9ring F,Wolffram S,Rimbach G.2011.Effect of quercetin and its metabolites isorhamnetin and quercetin-3-glucuronide on inflammatory gene expression:role of miR-155.Journal of Nutritional Biochemistry,22(3):293-299.
Boots A W,Haenen G R,Bast A.2008.Health effects of quercetin:from antioxidant to nutraceutical.European Journal of Pharmacology,585(2-3):325-337.
Byun E B,Yang M S,Choi H G,Sunga N Y,Songa D S,Sinc S J,Byun E H.2013.Quercetin negatively regulates TLR4 signaling induced by lipopolysaccharide through Tollip expression.Biochemical&Biophysical Research Communications,431(4):698-705.
Chandra R K.1997.Food hypersensitivity and allergic diseases:a selective review.American Journal of Clinical Nutrition,66(2):526S-529S.
Chen J F,Zhong W Q,Liu W,Li Y L,Wang D H.2007.Effects of dietary protein content on social behavior and some physiological properties in male Mongolian gerbils(Meriones unguiculatus).Acta Theriologica Sinica,27(3):234-242.(in Chinese)
Dai X,Zhang B,Wu X,Jiang L Y,Zou Z Z,Wang A Q,Wei W H,Yang S G.2014.Identification of tannin-degrading microorganisms in the gut of plateau pikas(Ochotona curzoniae)and root voles(Microtus oeconomus).Symbiosis,63(1):1-9.
Gong F L.2007.Medical Immunology.Beijing:Science Press,1-13,46-52.(in Chinese)
Grossman C J.1985.Interaction between the gonadal steroids and the immune system.Science,227(4684):257-261.
Gu H,Goodale E,Chen J.2015.Emerging directions in the study of the ecology and evolution of plant-animal mutualistic networks:a review.Zoological Research,36(2):65-71.
Guo J J,Sun W B.2012.Research progress of the IL-10 receptor and signal transduction.Clinical Medicine&Engineering,19(1):135-137.(in Chinese)
Hattas D,Hjltén J,Julkunen-Tiitto R,Scogings P F,Rooke T.2011.Differential phenolic profiles in six African savanna woody species in relation to antiherbivore defense.Phytochem,72(14-15):1796-1803.
He L,Li J N,Yang D M,Tao S L.2010.Effects of plant phenolic compound on the growth and organ development of reed voles(Microtus fortis).Acta Theriologica Sinica,30(3):297-303.(in Chinese)
Hochman V,Kotler B P.2006.Effects of food quality,diet preference and water on patch use by Nubian ibex.Oikos,112(3):547-554.
Katsanis A,Rasmann S,Mooney K A.2016.Herbivore diet breadth and host plant defense mediate the tri-trophic effects of plant toxins on multiple coccinellid predators.PLoS ONE,11(5):1-17.
Lamy E,Graa G,Da C G,Franco C E,Silva F C,Baptista E S,Coelho A V.2010.Changes in mouse whole saliva soluble proteome induced by tannin-enriched diet.Proteome Science,8(1):65-77.
Lee K W,Kang N J,Heo Y S,Rogozin E A,P Angelo,Mun K H,Bowden G T,Bode A M,Lee H J,Dong Z G.2008.Raf and MEKprotein kinases are direct molecular targets for the chemopreventive effect of quercetin,a major flavonol in red wine.Cancer Research,68(3):946-955.
Li J N,Liu J K,Tao S L.2007.Effects of hunger and tannic acid on food intake and foraging behaviors in Microtus fortis.Acta Ecologica Sinica,27(11):4478-4484.(in Chinese)
Li Q Y,Xu H Y,Yang H J.2017.Effect of proinflammatory factors TNF-α,IL-1β,IL-6 on neuropathic pain.China Journal of Chinese Materia Medica,(19):3709-3712.(in Chinese)
Lindroth R L,Batzli G O.1984.Plant phenolics as chemical defenses:Effects of natural phenolics on survival and growth of prairie voles(Microtus ochrogaster).Journal of Chemical Ecology,10(2):229-244.
Liu Z H,Zhang L.2016.Effects of total flavone extract from shuiqin(Oenanthe javanica).Chinese Journal of Traditional Medical Science and Technology,23(04):423-425.(in Chinese)
Lochmiller R L,Deerenberg C.2000.Trade-offs in evolutionary immunology:just what is the cost of immunity?Oikos,88(1):87-98.
Loke W M,Proudfoot J M,Mckinley A J,Needs P W,Kroon P A,Hodgson J M,Croft K D.2008.Quercetin and its in vivo metabolites inhibit neutrophil-mediated low-density lipoprotein oxidation.Journal of Agricultural&Food Chemistry,56(10):3609-3615.
Mansoori B,Nodeh H,Modirsanei M,Kiaei M M,Farkhoy M.2007.Influence of dietary tannic acid and polyethylene glycol on growth and intestinal D-xylose absorption of broiler cockerels and activity of serum enzymes.British Poultry Science,48(4):489-495.
Marsh K J,Wallis I R,Andrew R L,Foley W J.2006.The detoxification limitation hypothesis:where did it come from and where is it going?Journal of Chemical Ecology,32(6):1247-1266.
Mclean S,Duncan A J.2006.Pharmacological perspectives on the detoxification of plant secondary metabolites:implications for ingestive behavior of herbivores.Journal of Chemical Ecology,32(6):1213-1228.
Mella V.2014.The role of personality in the foraging decisions of a mammalian generalist herbivore,the common brushtail possum.University of Sydneyschool of Biological Sciences.
Meyer M W,Richardson C.1993.The effects of chronic tannic acid intake on prairie vole(Microtus ochrogaster)reproduction.Journal of Chemical Ecology,19(7):1577-1585.
Mlcek J,Jurikova T,Skrovankova S,Sochor J.2016.Quercetin and it’s anti-allergic immune response.Molecules,21(5):623-638.
Neznanov N,Kondratova A,Chumakov K M,Neznanova L,Kondratov R,Banerjee A K,Gudkov A V.2008.Quercetinase pirin makes poliovirus replication resistant to flavonoid quercetin.Dna&Cell Biology,27(4):191-198.
Ni W,Li J S,Yu Y.2017.Inhibitory effect ofquercetin on lipopolysaccharide induced inflammatory response in peritoneal macrophages of mice.Science and Technology of Food Industry,1(23):284-288.(in Chinese)
Perezvizcaino F,Duarte J,Andriantsitohaina R.2006.Endothelial function and cardiovascular disease:effects of quercetin and wine polyphenols.Free Radical Research,40(10):1054-1065.
Ritz B W,Gardner E M.2006.Malnutrition and energy restriction differentially affect viral immunity.Journal of Nutrition,136(5):1141-1144.
Shaik Y B,Castellani M L,Perrella A,Conti F,Salini V,Tete S,Madhappan B,Vecchiet J,De Lutiis M A,Caraffa A,Cerulli G.2006.Role of quercetin(a natural herbal compound)in allergy and inflammation.J Biol Regul Homeost Agents,20(3-4):47-52.
Stachowicz J J,Hay M E.2008.Reducing predation through chemically mediated camouflage:indirect efects of plant defenses on herbivores.Ecology,80(2):495-509.
Taylor A K,Gangappa S.2013.Protein energy malnutrition decreases immunity and increases susceptibility to influenza infection in mice.Journal of Infectious Diseases,207(3):501-510.
Villalba J J,Provenza F D.2005.Foraging in chemically diverse environments:energy,protein,and alternative foods influence ingestion of plant secondary metabolites by lambs.Journal of Chemical Ecology,31(1):123-138.
Wang Y,Gou C,Zhang M W,Li B,Chen G A.2004.Population dynamics of Microtus fortis in Dongting Lake region and its forecasting.Chinese Journal of Applied Ecology,15(2):308-312.(in Chinese)
Winter H,Engel N K V D,Rüttinger D,Schmidt J,Schiller M,Poehlein C H,L9he F,Fox B A,Jauch K W,Hatz R A,Hu H M.2007.Therapeutic T cells induce tumor-directed chemotaxis of innate immune cells through tumor-specific secretion of chemokines and stimulation of B16BL6 melanoma to secrete chemokines.Journal of Translational Medicine,5(1):56-69.
Zhai Z M.2013.IL-2 and its bidirectional regulatory effect on immune activation and immune tolerance.Chinese Pharmacological Bulletin,29(3):319-322.(in Chinese)
Zhu Y S,Zhang Y,Ning Z L,Wang R,Li X H,Zhao J Z.2008.Effects of isolavone extracted from alfalfa on growth performance and immune function in mice.Acta Nutrimenta Sinica,30(6):615-618.(in Chinese)
王勇,郭聪,张美文,李波,陈安国.2004.洞庭湖区东方田鼠种群动态及其危害预警.应用生态学报,15(2):308-312.
朱宇旌,张勇,宁自利,王蕊,李新华,赵加柱.2008.苜蓿异黄酮提取物对小鼠生长和免疫功能的影响.营养学报,30(6):615-618.
刘哲慧,张琳.2016.水芹总黄酮对免疫抑制小鼠免疫功能的影响.中国中医药科技,23(4):423-425.
李秋月,许海玉,杨洪军.2017.促炎因子TNF-α,IL-1β,IL-6在神经病理性疼痛中的研究进展.中国中药杂志,(19):3709-3712.
李俊年,刘季科,陶双伦.2007.饥饿和食物单宁酸对东方田鼠(Microtus fortis)食物摄入量和觅食行为的影响.生态学报,27(11):4478-4484.
何岚,李俊年,杨冬梅,陶双伦.2010.植物酚类化合物对东方田鼠体重增长和内脏器官发育的作用.兽类学报,30(3):297-303.
陈竞峰,钟文勤,刘伟,李玉梁,王德华.2007.食物蛋白含量对雄性长爪沙鼠社会行为和某些生理特征的影响.兽类学报,27(3):234-242.
倪湾,李敬双,于洋.2017.洋葱槲皮素对脂多糖诱导的小鼠腹腔巨噬细胞炎症反应抑制作用.食品工业科技,1(13):284-288.
郭锦锦,孙万邦.2012.IL-10受体及其信号转导研究进展.临床医学工程,19(1):135-137.
龚非力.2007.医学免疫学.北京:科学出版社,1-13,46-52.
翟志敏.2013.IL-2对免疫激活和免疫耐受的双向调节作用.中国药理学通报,298(3):319-322.
Angelika B,Jutta W,Martin A,Nicolas R,Humpert P M,Dimitri P,Roman F,Maximilian V E,Thoralf W,Gottfried R,Martina J,Michael M,Markus S,Bruce M,Clemens K,Peter P N.2003.Amechanism converting psychosocial stress into mononuclear cell activation.Proc Natl Acad Sci USA,100(4):1920-1925.
Bergeron J M,Jodoin L,Jean Y.1987.Pathology voles(Microtus pennsylvanicus)fed with plant extracts.Journal of Mammalogy,68(1):73-79.
Boesch S C,Loboda A,Wagner A E,Stachurska A,Jozkowicz A,Dulak J,D9ring F,Wolffram S,Rimbach G.2011.Effect of quercetin and its metabolites isorhamnetin and quercetin-3-glucuronide on inflammatory gene expression:role of miR-155.Journal of Nutritional Biochemistry,22(3):293-299.
Boots A W,Haenen G R,Bast A.2008.Health effects of quercetin:from antioxidant to nutraceutical.European Journal of Pharmacology,585(2-3):325-337.
Byun E B,Yang M S,Choi H G,Sunga N Y,Songa D S,Sinc S J,Byun E H.2013.Quercetin negatively regulates TLR4 signaling induced by lipopolysaccharide through Tollip expression.Biochemical&Biophysical Research Communications,431(4):698-705.
Chandra R K.1997.Food hypersensitivity and allergic diseases:a selective review.American Journal of Clinical Nutrition,66(2):526S-529S.
Chen J F,Zhong W Q,Liu W,Li Y L,Wang D H.2007.Effects of dietary protein content on social behavior and some physiological properties in male Mongolian gerbils(Meriones unguiculatus).Acta Theriologica Sinica,27(3):234-242.(in Chinese)
Dai X,Zhang B,Wu X,Jiang L Y,Zou Z Z,Wang A Q,Wei W H,Yang S G.2014.Identification of tannin-degrading microorganisms in the gut of plateau pikas(Ochotona curzoniae)and root voles(Microtus oeconomus).Symbiosis,63(1):1-9.
Gong F L.2007.Medical Immunology.Beijing:Science Press,1-13,46-52.(in Chinese)
Grossman C J.1985.Interaction between the gonadal steroids and the immune system.Science,227(4684):257-261.
Gu H,Goodale E,Chen J.2015.Emerging directions in the study of the ecology and evolution of plant-animal mutualistic networks:a review.Zoological Research,36(2):65-71.
Guo J J,Sun W B.2012.Research progress of the IL-10 receptor and signal transduction.Clinical Medicine&Engineering,19(1):135-137.(in Chinese)
Hattas D,Hjltén J,Julkunen-Tiitto R,Scogings P F,Rooke T.2011.Differential phenolic profiles in six African savanna woody species in relation to antiherbivore defense.Phytochem,72(14-15):1796-1803.
He L,Li J N,Yang D M,Tao S L.2010.Effects of plant phenolic compound on the growth and organ development of reed voles(Microtus fortis).Acta Theriologica Sinica,30(3):297-303.(in Chinese)
Hochman V,Kotler B P.2006.Effects of food quality,diet preference and water on patch use by Nubian ibex.Oikos,112(3):547-554.
Katsanis A,Rasmann S,Mooney K A.2016.Herbivore diet breadth and host plant defense mediate the tri-trophic effects of plant toxins on multiple coccinellid predators.PLoS ONE,11(5):1-17.
Lamy E,Graa G,Da C G,Franco C E,Silva F C,Baptista E S,Coelho A V.2010.Changes in mouse whole saliva soluble proteome induced by tannin-enriched diet.Proteome Science,8(1):65-77.
Lee K W,Kang N J,Heo Y S,Rogozin E A,P Angelo,Mun K H,Bowden G T,Bode A M,Lee H J,Dong Z G.2008.Raf and MEKprotein kinases are direct molecular targets for the chemopreventive effect of quercetin,a major flavonol in red wine.Cancer Research,68(3):946-955.
Li J N,Liu J K,Tao S L.2007.Effects of hunger and tannic acid on food intake and foraging behaviors in Microtus fortis.Acta Ecologica Sinica,27(11):4478-4484.(in Chinese)
Li Q Y,Xu H Y,Yang H J.2017.Effect of proinflammatory factors TNF-α,IL-1β,IL-6 on neuropathic pain.China Journal of Chinese Materia Medica,(19):3709-3712.(in Chinese)
Lindroth R L,Batzli G O.1984.Plant phenolics as chemical defenses:Effects of natural phenolics on survival and growth of prairie voles(Microtus ochrogaster).Journal of Chemical Ecology,10(2):229-244.
Liu Z H,Zhang L.2016.Effects of total flavone extract from shuiqin(Oenanthe javanica).Chinese Journal of Traditional Medical Science and Technology,23(04):423-425.(in Chinese)
Lochmiller R L,Deerenberg C.2000.Trade-offs in evolutionary immunology:just what is the cost of immunity?Oikos,88(1):87-98.
Loke W M,Proudfoot J M,Mckinley A J,Needs P W,Kroon P A,Hodgson J M,Croft K D.2008.Quercetin and its in vivo metabolites inhibit neutrophil-mediated low-density lipoprotein oxidation.Journal of Agricultural&Food Chemistry,56(10):3609-3615.
Mansoori B,Nodeh H,Modirsanei M,Kiaei M M,Farkhoy M.2007.Influence of dietary tannic acid and polyethylene glycol on growth and intestinal D-xylose absorption of broiler cockerels and activity of serum enzymes.British Poultry Science,48(4):489-495.
Marsh K J,Wallis I R,Andrew R L,Foley W J.2006.The detoxification limitation hypothesis:where did it come from and where is it going?Journal of Chemical Ecology,32(6):1247-1266.
Mclean S,Duncan A J.2006.Pharmacological perspectives on the detoxification of plant secondary metabolites:implications for ingestive behavior of herbivores.Journal of Chemical Ecology,32(6):1213-1228.
Mella V.2014.The role of personality in the foraging decisions of a mammalian generalist herbivore,the common brushtail possum.University of Sydneyschool of Biological Sciences.
Meyer M W,Richardson C.1993.The effects of chronic tannic acid intake on prairie vole(Microtus ochrogaster)reproduction.Journal of Chemical Ecology,19(7):1577-1585.
Mlcek J,Jurikova T,Skrovankova S,Sochor J.2016.Quercetin and it’s anti-allergic immune response.Molecules,21(5):623-638.
Neznanov N,Kondratova A,Chumakov K M,Neznanova L,Kondratov R,Banerjee A K,Gudkov A V.2008.Quercetinase pirin makes poliovirus replication resistant to flavonoid quercetin.Dna&Cell Biology,27(4):191-198.
Ni W,Li J S,Yu Y.2017.Inhibitory effect ofquercetin on lipopolysaccharide induced inflammatory response in peritoneal macrophages of mice.Science and Technology of Food Industry,1(23):284-288.(in Chinese)
Perezvizcaino F,Duarte J,Andriantsitohaina R.2006.Endothelial function and cardiovascular disease:effects of quercetin and wine polyphenols.Free Radical Research,40(10):1054-1065.
Ritz B W,Gardner E M.2006.Malnutrition and energy restriction differentially affect viral immunity.Journal of Nutrition,136(5):1141-1144.
Shaik Y B,Castellani M L,Perrella A,Conti F,Salini V,Tete S,Madhappan B,Vecchiet J,De Lutiis M A,Caraffa A,Cerulli G.2006.Role of quercetin(a natural herbal compound)in allergy and inflammation.J Biol Regul Homeost Agents,20(3-4):47-52.
Stachowicz J J,Hay M E.2008.Reducing predation through chemically mediated camouflage:indirect efects of plant defenses on herbivores.Ecology,80(2):495-509.
Taylor A K,Gangappa S.2013.Protein energy malnutrition decreases immunity and increases susceptibility to influenza infection in mice.Journal of Infectious Diseases,207(3):501-510.
Villalba J J,Provenza F D.2005.Foraging in chemically diverse environments:energy,protein,and alternative foods influence ingestion of plant secondary metabolites by lambs.Journal of Chemical Ecology,31(1):123-138.
Wang Y,Gou C,Zhang M W,Li B,Chen G A.2004.Population dynamics of Microtus fortis in Dongting Lake region and its forecasting.Chinese Journal of Applied Ecology,15(2):308-312.(in Chinese)
Winter H,Engel N K V D,Rüttinger D,Schmidt J,Schiller M,Poehlein C H,L9he F,Fox B A,Jauch K W,Hatz R A,Hu H M.2007.Therapeutic T cells induce tumor-directed chemotaxis of innate immune cells through tumor-specific secretion of chemokines and stimulation of B16BL6 melanoma to secrete chemokines.Journal of Translational Medicine,5(1):56-69.
Zhai Z M.2013.IL-2 and its bidirectional regulatory effect on immune activation and immune tolerance.Chinese Pharmacological Bulletin,29(3):319-322.(in Chinese)
Zhu Y S,Zhang Y,Ning Z L,Wang R,Li X H,Zhao J Z.2008.Effects of isolavone extracted from alfalfa on growth performance and immune function in mice.Acta Nutrimenta Sinica,30(6):615-618.(in Chinese)
王勇,郭聪,张美文,李波,陈安国.2004.洞庭湖区东方田鼠种群动态及其危害预警.应用生态学报,15(2):308-312.
朱宇旌,张勇,宁自利,王蕊,李新华,赵加柱.2008.苜蓿异黄酮提取物对小鼠生长和免疫功能的影响.营养学报,30(6):615-618.
刘哲慧,张琳.2016.水芹总黄酮对免疫抑制小鼠免疫功能的影响.中国中医药科技,23(4):423-425.
李秋月,许海玉,杨洪军.2017.促炎因子TNF-α,IL-1β,IL-6在神经病理性疼痛中的研究进展.中国中药杂志,(19):3709-3712.
李俊年,刘季科,陶双伦.2007.饥饿和食物单宁酸对东方田鼠(Microtus fortis)食物摄入量和觅食行为的影响.生态学报,27(11):4478-4484.
何岚,李俊年,杨冬梅,陶双伦.2010.植物酚类化合物对东方田鼠体重增长和内脏器官发育的作用.兽类学报,30(3):297-303.
陈竞峰,钟文勤,刘伟,李玉梁,王德华.2007.食物蛋白含量对雄性长爪沙鼠社会行为和某些生理特征的影响.兽类学报,27(3):234-242.
倪湾,李敬双,于洋.2017.洋葱槲皮素对脂多糖诱导的小鼠腹腔巨噬细胞炎症反应抑制作用.食品工业科技,1(13):284-288.
郭锦锦,孙万邦.2012.IL-10受体及其信号转导研究进展.临床医学工程,19(1):135-137.
龚非力.2007.医学免疫学.北京:科学出版社,1-13,46-52.
翟志敏.2013.IL-2对免疫激活和免疫耐受的双向调节作用.中国药理学通报,298(3):319-322.