辅助繁殖技术研究和应用进展
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摘要
动物繁殖是动物生产中的关键环节,辅助繁殖技术对提高动物的繁殖性能发挥着越来越重要的作用,其研究成果已广泛地应用于各领域中,为现代畜牧业的发展提供了更加广阔的前景,产生了深远的影响。文章主要阐述了人工授精及精液冷冻保存、定时输精技术、卵泡浆内精子注射、卵母细胞和胚胎冷冻保存、合子和配子输卵管内移植、纳米技术、激光技术等七个方面的动物辅助繁殖技术研究领域取得的成就、新的研究热点和存在问题,以期为相关研究和应用提供理论参考。
Animal reproduction was the key component during animal production,and assisted reproduction technology played an increasingly important role in improving animal reproductive performance. The research findings had been widely used in various fields,provided broader prospects for the development of modern animal husbandry,and took a far-reaching effect. The achievements,new hotspots and existing problems of seven aspects in this paper were mainly elaborated,including artificial insemination and semen cryopreservation,timed artificial insemination,intra-cytoplasmic sperm injection,oocyte and embryo cryopreservation,zygote and gamete intra-fallopian transfer,nanotechnology,and laser technology. This could provide the theoretical reference for related research and application.
Animal reproduction was the key component during animal production,and assisted reproduction technology played an increasingly important role in improving animal reproductive performance. The research findings had been widely used in various fields,provided broader prospects for the development of modern animal husbandry,and took a far-reaching effect. The achievements,new hotspots and existing problems of seven aspects in this paper were mainly elaborated,including artificial insemination and semen cryopreservation,timed artificial insemination,intra-cytoplasmic sperm injection,oocyte and embryo cryopreservation,zygote and gamete intra-fallopian transfer,nanotechnology,and laser technology. This could provide the theoretical reference for related research and application.
引文
[1]吴俊辉,曹丁壬,刘艳婷,等.家畜定时输精技术研究进展与应用现状[J].中国畜牧兽医,2016,43(7):1826-1833.
[2]徐振军,赵力新,刘冰,等.冻融过程对绵羊精子ATP酶活性的影响[J].黑龙江畜牧兽医,2016(07下):82-84.
[3]薛晓蓉,杨桂梅,胡宁玺,等.藏獒精液稀释液的筛选实验[J].中国畜牧杂志,2017,53(2):55-57.
[4]CHANAPIWAT P,KAEOKET K,TUMMARUK P.Cryopreservation of boar sperm by egg yolk-based extenders Containing lactose or fructose is better than sorbitol[J].J Vet Med Sci,2012,74(3):351-354.
[5]查星琴,肖晶,成文敏,等.版纳微型猪近交系精子冷冻保存研究[J].中国畜牧兽医,2017,44(1):167-172.
[6]权国波,吴国权,吕春荣,等.抗冻蛋白对山羊精子冷冻保护效果的分析[J].中国畜牧杂志,2017,53(3):53-57.
[7]DE VASCONCELOS FRANCO J S,FAHEEM M,CHAVEIRO A,et al.Effects ofα-tocopherol and freezing rates on the quality and heterologous in vitro fertilization capacity of stallion sperm after cryopreservation[J].Theriogenology,2016,86(4):957-962.
[8]杨莉,李海静,姜桂苗,等.精液剂型、输精部位对母驴情期受胎率的影响[J].黑龙江畜牧兽医,2017(02下):72-74.
[9]陈映,朱淋,杨露,等.不同输精方式和不同精液类型对母猪繁殖性能的影响[J].中国畜牧杂志,2017,53(4):59-63.
[10]COLAZO M G,MAPLETOFT R J.A review of current timed-AI(TAI)programs for beef and dairy cattle[J].Can Vet J,2014,55(8):772-780.
[11]傅春泉,陈龙,季春雷.定时输精技术在规模奶牛场的应用[J].畜牧与兽医,2013,45(4):34-37.
[12]李文瑞,田树军,薛振卫,等.奶牛定时输精技术研究[J].中国畜牧杂志,2017,53(3):63-66.
[13]UEHARA T,YANAGIMACHI R.Microsurgical injection of spermatozoa into hamster eggs with subsequent transformation of sperm nuclei into male pronuclei[J].Biol Reprod,1976,15(4):467-470.
[14]周小梅.ICSI技术挽救GFP兔及介导家兔转基因的研究[D].哈尔滨:东北林业大学,2014.
[15]曹俊国,魏海军,许保增.哺乳动物生殖细胞冷冻保存对其DNA甲基化影响的研究进展[J].中国畜牧兽医,2017,44(3):819-824.
[16]PARNPAI R,LIANG Y,KETUDAT-CAIRNS M,et al.Vitrification of buffalo oocytes and embryos[J].Theriogenology,2016,86(1):214-220.
[17]NAGY Z P,NEL-THEMAAT L,CHANG C C,et al.Cryopreservation of eggs[J].Methods Mol Biol,2014,1154:439-454.
[18]MANDAWALA A A,HARVEY S C,ROY T K,et al.Cryopreservation of animal oocytes and embryos:Current progress and future prospects[J].Theriogenology,2016,86(7):1637-1644.
[19]徐志鹏,王公金,张锡然,等.山羊配子输卵管内移植受精技术[J].江苏农业学报,2002,18(4):233-235.
[20]SCHUSTER T G,CHO B,KELLER L M,et al.Isolation of motile spermatozoa from semen samples using microfluidics[J].Reprod Biomed Online,2003,7(1):75-81.
[21]SUH R S,ZHU X,PHADKE N,et al.IVF within microfluidic channels requires lower total numbers and lower concentrations of sperm[J].Hum Reprod,2005,21(2):477-483.
[22]HUANG H Y,WU T L,HUANG H R,et al.Isolation of motile spermatozoa with a microfluidic chip having a surface-modified microchannel[J].J Lab Autom,2014,19(1):91-99.
[23]SHIROTA K,YOTSUMOTO F,ITOH H,et al.Separation efficiency of a microfluidic sperm sorter to minimize sperm DNA damage[J].Fertil Steril,2016,105(2):315-321.
[24]BEEBE D,WHEELER M,ZERINGUE H,et al.Microfluidic technology for assisted reproduction[J].Theriogenology,2002,57(1):125-135.
[25]GLASGOW I K,ZERINGUE H C,BEEBE D J,et al.Handling individual mammalian embryos using microfluidics[J].IEEE Trans Biomed Eng,2001,48(5):570-578.
[26]O’CONNELL M J,BACHILO S M,HUFFMAN C B,et al.Band gap fluorescence from individual single-walled carbon nanotubes[J].Science,2002,297(5581):593-596.
[27]ABDEL-SALAM Z,HARITH M A.Laser researches on livestock semen and oocytes:A brief review[J].J Adv Res,2014,6(3):311-317.
[28]FERNANDES G H,DE C P T,SERRA A J,et al.The effect of low-level laser irradiation on sperm motility,and integrity of the plasma membrane and acrosome in cryopreserved bovine sperm[J].PLo S One,2015,10(3):0121487.
[29]YESTE M,CODONF F,ESTRADA E,et al.Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates[J].Sci Rep,2016(6):22569.
[2]徐振军,赵力新,刘冰,等.冻融过程对绵羊精子ATP酶活性的影响[J].黑龙江畜牧兽医,2016(07下):82-84.
[3]薛晓蓉,杨桂梅,胡宁玺,等.藏獒精液稀释液的筛选实验[J].中国畜牧杂志,2017,53(2):55-57.
[4]CHANAPIWAT P,KAEOKET K,TUMMARUK P.Cryopreservation of boar sperm by egg yolk-based extenders Containing lactose or fructose is better than sorbitol[J].J Vet Med Sci,2012,74(3):351-354.
[5]查星琴,肖晶,成文敏,等.版纳微型猪近交系精子冷冻保存研究[J].中国畜牧兽医,2017,44(1):167-172.
[6]权国波,吴国权,吕春荣,等.抗冻蛋白对山羊精子冷冻保护效果的分析[J].中国畜牧杂志,2017,53(3):53-57.
[7]DE VASCONCELOS FRANCO J S,FAHEEM M,CHAVEIRO A,et al.Effects ofα-tocopherol and freezing rates on the quality and heterologous in vitro fertilization capacity of stallion sperm after cryopreservation[J].Theriogenology,2016,86(4):957-962.
[8]杨莉,李海静,姜桂苗,等.精液剂型、输精部位对母驴情期受胎率的影响[J].黑龙江畜牧兽医,2017(02下):72-74.
[9]陈映,朱淋,杨露,等.不同输精方式和不同精液类型对母猪繁殖性能的影响[J].中国畜牧杂志,2017,53(4):59-63.
[10]COLAZO M G,MAPLETOFT R J.A review of current timed-AI(TAI)programs for beef and dairy cattle[J].Can Vet J,2014,55(8):772-780.
[11]傅春泉,陈龙,季春雷.定时输精技术在规模奶牛场的应用[J].畜牧与兽医,2013,45(4):34-37.
[12]李文瑞,田树军,薛振卫,等.奶牛定时输精技术研究[J].中国畜牧杂志,2017,53(3):63-66.
[13]UEHARA T,YANAGIMACHI R.Microsurgical injection of spermatozoa into hamster eggs with subsequent transformation of sperm nuclei into male pronuclei[J].Biol Reprod,1976,15(4):467-470.
[14]周小梅.ICSI技术挽救GFP兔及介导家兔转基因的研究[D].哈尔滨:东北林业大学,2014.
[15]曹俊国,魏海军,许保增.哺乳动物生殖细胞冷冻保存对其DNA甲基化影响的研究进展[J].中国畜牧兽医,2017,44(3):819-824.
[16]PARNPAI R,LIANG Y,KETUDAT-CAIRNS M,et al.Vitrification of buffalo oocytes and embryos[J].Theriogenology,2016,86(1):214-220.
[17]NAGY Z P,NEL-THEMAAT L,CHANG C C,et al.Cryopreservation of eggs[J].Methods Mol Biol,2014,1154:439-454.
[18]MANDAWALA A A,HARVEY S C,ROY T K,et al.Cryopreservation of animal oocytes and embryos:Current progress and future prospects[J].Theriogenology,2016,86(7):1637-1644.
[19]徐志鹏,王公金,张锡然,等.山羊配子输卵管内移植受精技术[J].江苏农业学报,2002,18(4):233-235.
[20]SCHUSTER T G,CHO B,KELLER L M,et al.Isolation of motile spermatozoa from semen samples using microfluidics[J].Reprod Biomed Online,2003,7(1):75-81.
[21]SUH R S,ZHU X,PHADKE N,et al.IVF within microfluidic channels requires lower total numbers and lower concentrations of sperm[J].Hum Reprod,2005,21(2):477-483.
[22]HUANG H Y,WU T L,HUANG H R,et al.Isolation of motile spermatozoa with a microfluidic chip having a surface-modified microchannel[J].J Lab Autom,2014,19(1):91-99.
[23]SHIROTA K,YOTSUMOTO F,ITOH H,et al.Separation efficiency of a microfluidic sperm sorter to minimize sperm DNA damage[J].Fertil Steril,2016,105(2):315-321.
[24]BEEBE D,WHEELER M,ZERINGUE H,et al.Microfluidic technology for assisted reproduction[J].Theriogenology,2002,57(1):125-135.
[25]GLASGOW I K,ZERINGUE H C,BEEBE D J,et al.Handling individual mammalian embryos using microfluidics[J].IEEE Trans Biomed Eng,2001,48(5):570-578.
[26]O’CONNELL M J,BACHILO S M,HUFFMAN C B,et al.Band gap fluorescence from individual single-walled carbon nanotubes[J].Science,2002,297(5581):593-596.
[27]ABDEL-SALAM Z,HARITH M A.Laser researches on livestock semen and oocytes:A brief review[J].J Adv Res,2014,6(3):311-317.
[28]FERNANDES G H,DE C P T,SERRA A J,et al.The effect of low-level laser irradiation on sperm motility,and integrity of the plasma membrane and acrosome in cryopreserved bovine sperm[J].PLo S One,2015,10(3):0121487.
[29]YESTE M,CODONF F,ESTRADA E,et al.Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates[J].Sci Rep,2016(6):22569.