四川部分区域鸽源沙门菌携带情况的调查及其部分生物学特性的分析
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摘要
为了解四川部分区域鸽源沙门菌的病原携带情况及分离菌的部分生物学特性,笔者采用RTPCR对四川部分区域采集的1 375羽赛鸽、信鸽的肛门拭子、粪便样本开展了沙门菌携带情况的调查,检测分离鉴定得到的沙门菌部分毒力基因。结果鉴定出52株沙门菌,检出率为3.85%(95%置信区间为2.81%～4.85%),以双流区、郫都检出率较高,分别为11.23%(9/81)和11.11%(10/89),各区域间检出率差异极显著(P<0.01)。甲型副伤寒沙门菌、纽波特沙门菌均占定型菌株的11.9%,定型菌毒力基因sipC占比为92.85%,提示分离株有较强的入侵非吞噬细胞的能力。结果丰富了鸽源沙门菌携带情况的相关资料,为鸽源沙门菌的深入研究及防控提供了参考。
This study was conducted to learn the situation of Salmonella carrying status and some of their biological characteristics in pigeons in part of Sichuan Province.The total number of 1 375 pigeon fecal samples were collected from part of Sichuan Province and virulence genes of the isolates were detected.In result,the positive rate of Salmonella was 3.85%(95% confidence interval=2.81%-4.85%) in 1 375 pigeon fecal samples,sum to 52 strain.The positive rates were significantly different(P<0.01) among areas of the part of Sichuan Province,meanwhile the positive rates in Shuangliu District and Pixian County are higher than the other areas,the former is 11.23%(9/81) and the latter is 11.11%(10/89),respectively.11.9% of the isolates were Salmonella Paratyphi A or Newport.92.85%of the isolates had virulence gene sip C,indicating that the isolates have a strong ability to inter non-phagocytic cell.The above-mentioned results enrich the information on the pigeon-origin Salmonella and provide the theoretical basis for study on Salmonella in pigeons.
This study was conducted to learn the situation of Salmonella carrying status and some of their biological characteristics in pigeons in part of Sichuan Province.The total number of 1 375 pigeon fecal samples were collected from part of Sichuan Province and virulence genes of the isolates were detected.In result,the positive rate of Salmonella was 3.85%(95% confidence interval=2.81%-4.85%) in 1 375 pigeon fecal samples,sum to 52 strain.The positive rates were significantly different(P<0.01) among areas of the part of Sichuan Province,meanwhile the positive rates in Shuangliu District and Pixian County are higher than the other areas,the former is 11.23%(9/81) and the latter is 11.11%(10/89),respectively.11.9% of the isolates were Salmonella Paratyphi A or Newport.92.85%of the isolates had virulence gene sip C,indicating that the isolates have a strong ability to inter non-phagocytic cell.The above-mentioned results enrich the information on the pigeon-origin Salmonella and provide the theoretical basis for study on Salmonella in pigeons.
引文
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[18]孙璐,王娟,黄秀梅,等.山东地区肉鸡屠宰环节沙门菌流行病学调查与毒力分析[J].中国家禽,2015,37(7):67-70.SUN Lu,WANG Juan,HUANG Xiu-mei,et al.Epidemiological investigation and analysis of virulence genes of Salmonella in chicken from abattoir in Shangdong Province[J].China Poultry,2015,37(7):67-70.(in Chinese)
[19]DUTTA S,DAS S,MITRA U,et al.Antimicrobial resistance,virulence profiles and molecular subtypes of Salmonella enterica serovars typhi and paratyphi A blood isolates from Kolkata,India during2009-2013[J].PloS One,2014,9(8):e101347.
[20]MCWHORTER A R,CHOUSALKAR K K.Comparative phenotypic and genotypic virulence of Salmonella strains isolated from Australian layer farms[J].Frontiers in Microbiology,2015(6):12.
[21]陈俊,蒋文灿,程平,等.四川和重庆地区鸭源沙门氏菌分离鉴定及血清型分析[J].中国预防兽医学报,2015,37(4):262-265.CHEN Jun,JIANG Wen-can,CHENG Ping,et al.Isolation,identification and serotype analysis of Salmonella from ducklings and dead duck-embryos in Sichuan and Chongqing[J].Chinese Journal of Preventive Veterinary Medicine,2015,37(4):262-265.(in Chinese)
[22]郭伟娜,路振香,刘利晓,等.滁州活禽市场鸡源沙门菌的分离鉴定与药敏试验[J].动物医学进展,2017,38(6):107-111.GUO Wei-na,LU Zhen-xiang,LIU Li-xiao,et al.Isolation,identification and drug susceptibility test of Salmonella from chicken in live poultry market of Chuzhou[J].Progress in Veterinary Medicine,2017,38(6):107-111.(in Chinese)
[23]HULAJ B,?ABELI P,GOGA I,et al.Survey of the prevalence of Salmonella species on laying hen farms in Kosovo[J].Poultry Science,2016,95(9):2030-2037.
[24]沙依染古丽,成进,谷文喜,等.新疆地区鸡伤寒白痢沙门氏菌病的调查[J].中国预防兽医学报,2001,23(5):386-389.SARERANGULI,CEHNG Jin,GU Wen-xi,et al.Epidemiological investigation of S.gallinarum and S.pullorum in XinJiang[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(5):386-389.(in Chinese)
[25]SASAKI Y,MURAKAMI M,MARUYAMA N,et al.Risk factors for Salmonella prevalence in laying-hen farms in Japan[J].Epidemiology and Infection,2012,140(6):982-990.
[26]TANAKA C,MIYAZAWA T,WATARAI M,et al.Bacteriological survey of feces from feral pigeons in Japan[J].The Journal of Veterinary Medical Science,2005,67(9):951-953.
[27]OSMAN K M,MEHREZ M,ERFAN A M,et al.Salmonella enterica isolated from pigeon(Columba livia)in Egypt[J].Foodborne Pathogens Disease,2013,10(5):481-483.
[28]SASKIA V D,BRUINS M J,RUIJS G J H M.Evaluation and implementation of a chromogenic agar medium for Salmonella detection in stool in routine laboratory diagnostics[J].Journal of Clinical Microbiology,2009,47(47):456-458.
[29]WILSON G.Rapid and economical method for biochemical screening of stool isolates for Salmonella and Shigella species[J].Journal of Clinical Microbiology,2004,42(10):4821-4823.
[30]PEREZ J M,CAVALLI P,ROURE C,et al.Comparison of four chromogenic media and hektoen agar for detection and presumptive identification of Salmonella strains in human stools[J].Journal of Clinical Microbiology,2003,41(3):1130-1134.
[31]李茂辉.吉林省部分规模化猪场临床健康猪群沙门菌流行病学调查及耐药性分析[D].长春:吉林农业大学,2014.LI Mao-hui.The epidemiological suvey and analysis of drug resistance of Salmonella from clinical healthy pigs from part of scale pig farms in Jilin[D].Changchun:Jilin Agricultural University,2014.(in Chinese)
[32]张斌,朱晓霞,岳华,等.青藏高原部分地区牦牛源沙门菌血清型及毒力基因的调查[J].畜牧兽医学报,2013,44(7):1167-1172.ZAHNG Bin,ZHU Xiao-xia,YUE Hua,et al.The survey study on the serovar and virulent gene of yak Salmonella from some areas of Qinghai-Tibetan plateau[J].Chinese Journal of Animal and Veterinary Sciences,2013,44(7):1167-1172.(in Chinese)
[33]FIERER J,KRAUSE M,TAUXE R,et al.Salmonella typhimurium bacteremia:association with the virulence plasmid[J].Journal of Infectious Diseases,1992,166(3):639-642.
[34]黄道超,姜波,杨光友,等.凹甲陆龟沙门氏菌的分离鉴定及毒力因子基因的PCR检测[J].生物技术通报,2009(10):156-160.HUANG Dao-chao,JIANG Bo,YANG Guang-you,et al.Isolation and identification of Salmonella in manouria impressaand detection of virulence factor gene by PCR[J].Biotechnology Bulletin,2009(10):156-160.(in Chinese)
[35]杨柳,胡文忠,姜爱丽,等.分子生物学方法检测沙门氏菌的研究进展[J].食品工业科技,2016,37(9):372-375.YANG Liu,HU Wen-zhong,JIANG Ai-li,et al.Research progress in molecular biology methods for detection of Salmonella[J].Science and Technology of Food Industry,2016,37(9):372-375.(in Chinese)
[36]FàBREGA A,VILA J.Salmonella enterica serovar typhimurium skills to succeed in the host:virulence and regulation[J].Clinical Microbiology Reviews,2013,26(2):308-341.
[37]RAFFATELLU M,WILSON R P,CHESSA D,et al.SipA,SopA,SopB,SopD,and SopE2 contribute to Salmonella enterica serotype typhimurium invasion of epithelial cells[J].Infection and Immunity,2005,73(1):146-154.
[38]LI D,WANG X,WANG L,et al.The actin-polymerizing activity of SipA is not essential for Salmonella enterica serovar typhimurium-induced mucosal inflammation[J].Infection and Immunity,2013,81(5):1541-1549.
[2]SCHROEDER C M,NAUGLE A L,SCHLOSSER W D,et al.Estimate of illnesses from Salmonella enteritidis in eggs,United States,2000[J].Emerging Infectious Diseases,2005,11(1):113-115.
[3]PATRICK M E,ADCOCK P M,GOMEZ T M,et al.Salmonella enteritidis infections,United States,1985-1999[J].Emerging Infectious Diseases,2004,10(1):1-7.
[4]KIMURA AC,REDDY V,MARCUS R,et al.Chicken consumption is a newly identified risk factor for sporadic Salmonella enterica serotype enteritidis infections in the United States:a case-control study in FoodNet sites[J].Clinical Infectious Diseases,2004,38(3):244-252.
[5]VAN DE,WANNET W J,HOUWERS D J,et al.Serotype and phage type distribution of Salmonella strains isolated from humans,cattle,pigs,and chickens in the Netherlands from 1984 to 2001[J].Journal of Clinical Microbiology,2002,40(11):3980-3985.
[6]XIONG D,SONG L,GENG S,et al.One-step PCR detection of Salmonella pullorum/gallinarum using a novel target:the flagellar biosynthesis gene flhb[J].Frontiers in Microbiology,2016,7(178):1863.
[7]陈溥言.兽医传染病学[M].5版.北京:中国农业出版社,2006.CHEN Pu-yan.Veterinary Infectious Diseases[M].5th ed.Beijing:China Agriculture Press,2006.(in Chinese)
[8]罗青平,温国元,王红琳,等.中国鸽病的流行及防控现状[J].湖北农业科学,2015,54(12):2954-2956.LUO Qing-ping,WEN Guo-yuan,WANG Hong-lin,et al.The epidemic situation of pigeon disease and its prevention and control in China[J].Hubei Agriculture Sciences,2015,54(12):2954-2956.(in Chinese)
[9]DUFFY L,BARLOW R,FEGAN N,et al.Prevalence and serotypes of Salmonlla associated with goats at two Australian abattoirs[J].Letters in Applied Microbiology,2008,48(2):193-197.
[10]刘军军,张宏海,方艳红,等.合肥地区鸡沙门菌带菌情况调查及其血清型与基因型分析[J].中国微生态学杂志,2011,23(7):582-585,588.LIU Jun-jun,ZHANG Hong-hai,FANG Yan-hong,et al.Investigation on the carrying rate of Salmonella from nonsymptomatic chickens in Hefei and analysis of their serotype and genotype[J].Chinese Journal of Microecology,2011,23(7):582-585,588.(in Chinese)
[11]岳秀英,汪开毓.四川省2009-2014年鸡源沙门菌耐药性变迁的调查[J].中国兽医科学,2015,45(5):544-550.YUE Xiu-ying,WANG Kai-yu.Prevalence of antimicrobial resistance in Salmonella from chickens in Sichuan province during 2009-2014[J].Chinese Veterinary Science,2015,45(5):544-550.(in Chinese)
[12]塞弗Y M.禽病学[M].苏敬良,高福,索勋,译.12版.北京:中国农业出版社,2012.SAIF Y M.Diseases of Poultry[M].Translated by SUJing-liang,GAO Fu,SUO Xun.12th ed.Beijing:China Agriculture Press,2012.(in Chinese)
[13]CARLI K T,EYIGOR A,CANER V.Prevalence of Salmonella serovars in chickens in Turkey[J].Journal of Food Protection,2001,64(11):1832-1835.
[14]SHIVAPRASAD H L.Fowl typhoid and pullorum disease[J].Revue Scientifique et Technique,2000,19(2):405.
[15]王琼,唐俊妮,汤承,等.一种采用微波炉加热快速提取细菌DNA用于PCR扩增的方法[J].西南民族大学学报(自然科学版),2015,41(2):150-155.WANG Qiong,TANG Jun-ni,TANG Cheng,et al.Rapid method with microwave oven heating for bacterial DNA extraction applied to PCR amplification[J].Journal of Southwest University(Natural Science Edition),2015,41(2):150-155.(in Chinese)
[16]SOTO S M,RODRIGUEZ I,RODICIO M R,et al.Detection of virulence determinants in clinical strains of Salmonella enterica serovar enteritidis and mapping on macrorestriction profiles[J].Journal of Medical Microbiology,2006,55(4):365-373.
[17]李幸,罗薇,刘内生,等.大雁和天鹅源沙门菌毒力基因分布的初步研究[J].动物医学进展,2014(3):28-32.LI Xing,LUO Wei,LIU Nei-sheng,et al.Preliminary study on distribution features of Salmonella virulence genes from Cygnus and Anser[J].Progress in Veterinary Medicine,2014(3):28-32.(in Chinese)
[18]孙璐,王娟,黄秀梅,等.山东地区肉鸡屠宰环节沙门菌流行病学调查与毒力分析[J].中国家禽,2015,37(7):67-70.SUN Lu,WANG Juan,HUANG Xiu-mei,et al.Epidemiological investigation and analysis of virulence genes of Salmonella in chicken from abattoir in Shangdong Province[J].China Poultry,2015,37(7):67-70.(in Chinese)
[19]DUTTA S,DAS S,MITRA U,et al.Antimicrobial resistance,virulence profiles and molecular subtypes of Salmonella enterica serovars typhi and paratyphi A blood isolates from Kolkata,India during2009-2013[J].PloS One,2014,9(8):e101347.
[20]MCWHORTER A R,CHOUSALKAR K K.Comparative phenotypic and genotypic virulence of Salmonella strains isolated from Australian layer farms[J].Frontiers in Microbiology,2015(6):12.
[21]陈俊,蒋文灿,程平,等.四川和重庆地区鸭源沙门氏菌分离鉴定及血清型分析[J].中国预防兽医学报,2015,37(4):262-265.CHEN Jun,JIANG Wen-can,CHENG Ping,et al.Isolation,identification and serotype analysis of Salmonella from ducklings and dead duck-embryos in Sichuan and Chongqing[J].Chinese Journal of Preventive Veterinary Medicine,2015,37(4):262-265.(in Chinese)
[22]郭伟娜,路振香,刘利晓,等.滁州活禽市场鸡源沙门菌的分离鉴定与药敏试验[J].动物医学进展,2017,38(6):107-111.GUO Wei-na,LU Zhen-xiang,LIU Li-xiao,et al.Isolation,identification and drug susceptibility test of Salmonella from chicken in live poultry market of Chuzhou[J].Progress in Veterinary Medicine,2017,38(6):107-111.(in Chinese)
[23]HULAJ B,?ABELI P,GOGA I,et al.Survey of the prevalence of Salmonella species on laying hen farms in Kosovo[J].Poultry Science,2016,95(9):2030-2037.
[24]沙依染古丽,成进,谷文喜,等.新疆地区鸡伤寒白痢沙门氏菌病的调查[J].中国预防兽医学报,2001,23(5):386-389.SARERANGULI,CEHNG Jin,GU Wen-xi,et al.Epidemiological investigation of S.gallinarum and S.pullorum in XinJiang[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(5):386-389.(in Chinese)
[25]SASAKI Y,MURAKAMI M,MARUYAMA N,et al.Risk factors for Salmonella prevalence in laying-hen farms in Japan[J].Epidemiology and Infection,2012,140(6):982-990.
[26]TANAKA C,MIYAZAWA T,WATARAI M,et al.Bacteriological survey of feces from feral pigeons in Japan[J].The Journal of Veterinary Medical Science,2005,67(9):951-953.
[27]OSMAN K M,MEHREZ M,ERFAN A M,et al.Salmonella enterica isolated from pigeon(Columba livia)in Egypt[J].Foodborne Pathogens Disease,2013,10(5):481-483.
[28]SASKIA V D,BRUINS M J,RUIJS G J H M.Evaluation and implementation of a chromogenic agar medium for Salmonella detection in stool in routine laboratory diagnostics[J].Journal of Clinical Microbiology,2009,47(47):456-458.
[29]WILSON G.Rapid and economical method for biochemical screening of stool isolates for Salmonella and Shigella species[J].Journal of Clinical Microbiology,2004,42(10):4821-4823.
[30]PEREZ J M,CAVALLI P,ROURE C,et al.Comparison of four chromogenic media and hektoen agar for detection and presumptive identification of Salmonella strains in human stools[J].Journal of Clinical Microbiology,2003,41(3):1130-1134.
[31]李茂辉.吉林省部分规模化猪场临床健康猪群沙门菌流行病学调查及耐药性分析[D].长春:吉林农业大学,2014.LI Mao-hui.The epidemiological suvey and analysis of drug resistance of Salmonella from clinical healthy pigs from part of scale pig farms in Jilin[D].Changchun:Jilin Agricultural University,2014.(in Chinese)
[32]张斌,朱晓霞,岳华,等.青藏高原部分地区牦牛源沙门菌血清型及毒力基因的调查[J].畜牧兽医学报,2013,44(7):1167-1172.ZAHNG Bin,ZHU Xiao-xia,YUE Hua,et al.The survey study on the serovar and virulent gene of yak Salmonella from some areas of Qinghai-Tibetan plateau[J].Chinese Journal of Animal and Veterinary Sciences,2013,44(7):1167-1172.(in Chinese)
[33]FIERER J,KRAUSE M,TAUXE R,et al.Salmonella typhimurium bacteremia:association with the virulence plasmid[J].Journal of Infectious Diseases,1992,166(3):639-642.
[34]黄道超,姜波,杨光友,等.凹甲陆龟沙门氏菌的分离鉴定及毒力因子基因的PCR检测[J].生物技术通报,2009(10):156-160.HUANG Dao-chao,JIANG Bo,YANG Guang-you,et al.Isolation and identification of Salmonella in manouria impressaand detection of virulence factor gene by PCR[J].Biotechnology Bulletin,2009(10):156-160.(in Chinese)
[35]杨柳,胡文忠,姜爱丽,等.分子生物学方法检测沙门氏菌的研究进展[J].食品工业科技,2016,37(9):372-375.YANG Liu,HU Wen-zhong,JIANG Ai-li,et al.Research progress in molecular biology methods for detection of Salmonella[J].Science and Technology of Food Industry,2016,37(9):372-375.(in Chinese)
[36]FàBREGA A,VILA J.Salmonella enterica serovar typhimurium skills to succeed in the host:virulence and regulation[J].Clinical Microbiology Reviews,2013,26(2):308-341.
[37]RAFFATELLU M,WILSON R P,CHESSA D,et al.SipA,SopA,SopB,SopD,and SopE2 contribute to Salmonella enterica serotype typhimurium invasion of epithelial cells[J].Infection and Immunity,2005,73(1):146-154.
[38]LI D,WANG X,WANG L,et al.The actin-polymerizing activity of SipA is not essential for Salmonella enterica serovar typhimurium-induced mucosal inflammation[J].Infection and Immunity,2013,81(5):1541-1549.