云南西双版纳试验站大气真菌群落的调查
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摘要
采用撞击法对热带雨林西双版纳大气试验站不同试验场地类型(试验场、试验棚、试验库)按干季和雨季共4次采集大气真菌,用统计学法计算出不同试验场地干季和雨季的真菌数量,采用真菌形态学方法,结合ITS1-5.8S-TIS2或26S rDNA D1/D2区序列分析法鉴定到种,并用SPSS和R软件初步探讨大气真菌与环境因素的关系。结果表明云南西双版纳试验站大气真菌浓度为2 200–4 300cfu/m3;从西双版纳大气试验站分离得到776株真菌鉴定为34属52个种,优势种群为尖孢枝孢菌Cladosporium oxysporum、变红镰孢菌Fusariumincarnatum、歧皱青霉菌Penicilliumsteckii、 Aspergillusminisclerotigenes、Leptosphaerulina chartarum、Clonostachys epichloe和Paraconiothyrium estuarinum;大气环境中真菌多样性较丰富,真菌群落和浓度随季节性变化;3个试验场地不同季节之间真菌的群落结构差异大,影响大气真菌群落结构的重要环境因素是降雨量和气压。
The impact method was used to collect airborne fungi in dry and rainy seasons for 4 times in different experimental sites(fields, sheds and storehouse) of the Xishuangbanna Tropical Rainforest Atmospheric Experiment Station. The quantity of fungi was calculated using a statistical method at different experimental sites in dry and rainy seasons. The fungal strains were identified by morphological characters combined with ITS1-5.8 S-TIS2 or 26 S rDNA D1/D2 region sequence analysis. The relationship between environmental factors and atmospheric fungi was preliminarily investigated using the SPSS and R software. The results showed that the concentration of airborne fungi was 2 200–4 300 cfu/m3 in the atmosphere ofXishuangbanna Experiment Station, and 776 strains of fungi were isolated and classified into 34 genera and 52 species. The dominant species were Aspergillus minisclerotigenes, Cladosporium oxysporum, Clonostachys epichloe, Fusarium incarnatum, Leptosphaerulina chartarum, Paraconiothyrium estuarinum and Penicillium steckii. The fungal diversity in the atmospheric environment is rich but the communities and concentrations of fungi changed seasonally. The community structure of fungi varied significantly among the three experimental sites in different seasons. The important environmental factors which affected the structure of airborne fungal community were precipitation and atmospheric pressure.
The impact method was used to collect airborne fungi in dry and rainy seasons for 4 times in different experimental sites(fields, sheds and storehouse) of the Xishuangbanna Tropical Rainforest Atmospheric Experiment Station. The quantity of fungi was calculated using a statistical method at different experimental sites in dry and rainy seasons. The fungal strains were identified by morphological characters combined with ITS1-5.8 S-TIS2 or 26 S rDNA D1/D2 region sequence analysis. The relationship between environmental factors and atmospheric fungi was preliminarily investigated using the SPSS and R software. The results showed that the concentration of airborne fungi was 2 200–4 300 cfu/m3 in the atmosphere ofXishuangbanna Experiment Station, and 776 strains of fungi were isolated and classified into 34 genera and 52 species. The dominant species were Aspergillus minisclerotigenes, Cladosporium oxysporum, Clonostachys epichloe, Fusarium incarnatum, Leptosphaerulina chartarum, Paraconiothyrium estuarinum and Penicillium steckii. The fungal diversity in the atmospheric environment is rich but the communities and concentrations of fungi changed seasonally. The community structure of fungi varied significantly among the three experimental sites in different seasons. The important environmental factors which affected the structure of airborne fungal community were precipitation and atmospheric pressure.
引文
Bai X,Shi YW,Dong XH,Xue J,Li P,Lou K,2013.Biodiversity of airborne cultivable fungi isolated from spring dust of Taklamakan Desert.Xinjiang Agricultural Sciences,50(7):1314-1321(in Chinese)
Barnett HL,Hunter BB,1977.Illustrated genera of imperfect fungi(third edition).Translated by Shen CY.Science Press,Beijing.1-240(in Chinese)
Beaumont F,Kauffman HF,Vandermark TH,Sluiter HJ,Devries K,1985.Volumetric aerobiological survey of conidial fungi in the North-East Netherlands.I.Seasonal patterns and the influence of meteorological variables.Allergy,40:173-180
Chen DM,Li JG,Su XR,Yang G,2006.Anti-mildew of military electronic equipment.Equipment Environmental Engineering,3(4):78-81,95(in Chinese)
de Leo F,Campanella G,Proverbio E,Urzi C,2013.Laboratory tests of fungal biocorrosion of unbonded lubricated post-tensioned tendons.Construction and Building Materials,49:821
Dong AR,Sun W,Wang TL,2008.Diversity of indoor airborne fungi in Harbin city.Journal of Northeast Forestry University,36(2):77-79(in Chinese)
Fang ZG,Huang C,Lou XQ,Li RZ,Yao WC,2017.Characteristics of airborne microbes in typical tourist city in Southeast China.China Environmental Science,37(8):2840-2847(in Chinese)
Fang ZG,Ouyang ZY,2009.Advance of airborne fungal community and the influencing factors in indoor and outdoor environments in urban ecosystem.Ecology and Environmental Sciences,18(1):386-393(in Chinese)
Garrett MH,Rayment PR,Hooper MA,Abramson MJ,Hooper BM,1998.Indoor airborne fungal spores,house dampness and associations with environmental factors and respiratory health in children.Clinical and Experimental Allergy,28(4):459-467
He XS,2015.Modern fungal classification system.Beijing Science Press,Beijing.1-627(in Chinese)
Hu LF,Fang ZG,Ouyang ZY,Liao XL,Lin XQ,Wang XK,2005.Ecological distributions of airborne fungi in outdoor environments in Beijing.Chinese Journal of Environmental Science,26(5):22-27(in Chinese)
Hu QX,Li JB,1994.Effect of solar radiation on the airborne fungi.Shanghai Environmental Sciences,13(2):19-21,32(in Chinese)
Huang JJ,Hong SL,Zhou W,Yang YC,2002.Air-borne molds in Chongqing city.Chongqing Medical Journal,31(8):701-703(in Chinese)
Ju TZ,Suo AM,Tian YJ,Feng KK,2003.Analysis on aerobiologia in Lanzhou.Industrial Safety and Environmental Protection,29(3):17-19
Kirk PM,Cannon PF,Minter DW,Stalpers JA,2008.Dictionary of the fungi.10th ed.CABI Publishing,Wallingford.1-784
Li CS,Kuo YM,1994.Characteristics of airborne microfungi in subtropical homes.Science of the Total Environment,155(3):267-271
Li DW,Kendrick B,1994.Functional relationships between airborne fungal spores and environmental factors in Kitchener-Waterloo,Ontario,as detect by canonical correspondence analysis.Grana,33:166-176
Lindmanm J,Constantinidou H,Barchet W,1982.Plants as sources of airborne bacteria,including ice nucleationaction bacteria.Grana,44(5):1059
Ling Q,Wang YP,Wang L,Shu Y,Tao Y,Bao LN,2008.Distribution of airborne fungi concentration in Hefei City,Anhui.Biodiversity Science,16(2):175-180(in Chinese)
Liu QQ,Lu L,Gao G,Li XG,2017.Research progress on fungal corrosion of metals and their protective layers in atmospheric environments.Journal of University of Science and Technology Beijing,39(10):1463-1469(in Chinese)
Liu SD,Kong WX,Wang J,2016.Investigation of typical marine mould corrosion environment-a case study of“DONG FANG HONG 2”.Guangzhou Chemical Industy,44(21):141-143(in Chinese)
Mouli C,Mohan S,Reddy S,2005.Assessment of microbial(bacteria)concentrations of ambient air at semi-arid urban region:influence of meteorological factors.Aeer,3:139-149
Nisiotou AA,Spriopoulos AE,Nychas GJ,2007.Yeast community structures and dynamics in healthy and bortytis-affected grape must fermentations.Applied and Environmental Microbiology,73(21):6705-6713
Ouyang YS,Chen YB,Xie XB,Huang XM,Chen JD,Peng H,Li JY,2003.Determination of airborne microbial cancentration around key traffic route in Guangzhou urbal districts.Chinses Journal of Health Laboratory Technology,13(6):692-693(in Chinese)
Peng WH,Bau T,2012.A review on community diversity of urban airborne fungi.Journal of Fungal Research,10(1):49-56(in Chinese)
Salvarezza RC,de Mele MFL,Videla HA,1983.Mechanisms of the microbial corrosion of aluminum alloys.Corrosion,39(1):26
Shao LP,Shen RX,Zhang SX,Xiang CT,Tan SS,1984.Fungal taxonomy.China Forestry Press,Beijing.1-370(in Chinese)
Song ZW,Wang L,Ren J,2014.Concentration distribution and characteristics of airborne microbes in spring and summer in Qingdao.Journal of Qingdao Technological University,35(3):1-6,23(in Chinese)
Tai FL,1987.Morphology and taxonomy of fungi.Science Press,Beijing.1-352(in Chinese)
Wei CT,1979.Fungal identification manual.Shanghai Science and Technology Press,Shanghai.1-780(in Chinese)
Wu P,Su H,Lin C,2000.Characteristics of indoor and outdoor airborne fungi at suburban and urban homes in two seasons.Science of the Total Environment,253:111-118
Zhang YL,Zheng SY,Zhi Q,Liu DB,2007.Detection of airborne fungus in different types of military underground construction.Journal of Environmental Occupational Medicine,24(4):454-455(in Chinese)
Zi JW,Zi YH,Tang H,Li Q,Zhang SM,Li XX,2015.Distribution and drug sensitivity of mountain air microorganisms at Deqin district in Yunnan Province.Medical Journal of National Defending Forces in Southwest China,25(10):1153-1156(in Chinese)
Zi JW,Zi YH,Tang H,Li Q,Zhang SM,Li XX,2016.Distribution and drug sensitivity of mountain air microorganisms at Shangri-La district in Yunnan Province.Journal of Preventive Medicine of Chinese People’s Liberation Army,34(2):159-161(in Chinese)
白雪,史应武,董秀黄,薛娟,李萍,娄恺,2013.塔克拉玛干沙漠西缘春季沙尘天气大气可培养真菌多样性.新疆农业科学,50(7):1314-1321
陈丹明,李金国,苏兴荣,杨光,2006.军用电子装备的防霉.装备环境工程,3(4):78-81,95
戴芳澜,1987.真菌的形态和分类.北京:科学出版社.1-352
董爱荣,孙巍,王天亮,2008.哈尔滨室内大气真菌多样性.东北林业大学学报,36(2):77-79
方治国,黄闯,楼秀芹,李睿哲,姚文冲,2017.南方典型旅游城市大气微生物特征研究.中国环境科学,37(8):2840-2847
方治国,欧阳志云,2009.城市室内外大气真菌群落及影响因素研究进展.生态环境学报,18(1):386-393
贺新生,2015.现代菌物分类系统.北京:科学出版社.1-627
H.L.巴尼特,B.B亨特(著),沈崇尧(译),1977.半知菌属图解(第三版).北京:科学出版社.1-240
胡利锋,方治国,欧阳志云,廖晓兰,林学强,王效科,2005.北京室外大气真菌分布特征.环境科学,26(5):22-27
胡庆轩,李军保,1994.日光辐射对大气真菌粒子的影响.上海环境科学,13(2):19-21,32
黄江菊,洪苏玲,周伟,杨玉成,2002.重庆城区大气中致敏真菌调查.重庆医学,31(8):701-703
凌琪,王晏平,王莉,舒莹,陶勇,鲍立宁,2008.合肥城区大气真菌浓度的时空分布特征.生物多样性,16(2):175-180
刘倩倩,卢琳,高歌,李晓刚,2017.大气环境中金属及其保护层霉菌腐蚀研究的进展.工程科学学报,39(10):1463-1469
刘士栋,孔维轩,王佳,2016.典型海洋霉菌腐蚀环境调查-“东方红2”为例.广州化工,44(21):141-143
欧阳友生,陈仪本,谢小保,黄小茉,陈娇娣,彭红,李剑英,2003.广州城区主要交通枢纽大气微生物浓度的测定.中国卫生检验杂志,13(6):692-693
彭炜航,图力古尔,2012.城市大气真菌群落多样性研究概况.菌物研究,10(1):49-56
邵力平,沈瑞祥,张素轩,项存悌,谭松山,1984.真菌分类学.北京:中国林业出版社.1-370
宋志文,王琳,任杰,2014.青岛市区春、夏季生物气溶胶浓度分布及特征.青岛理工大学学报,35(3):1-6,23
魏景超,1979.真菌鉴定手册.上海:上海科学技术出版社.1-780
张永良,郑世英,智强,刘东彪,2007.不同类型地下工事大气真菌检测分析.环境与职业医学,24(4):454-455
子建文,资云海,唐辉,李倩,张胜梅,李晓霞,2015.云南德钦地区山脉大气微生物分布及药敏情况.西南国防医药,25(10):1153-1156
子建文,资云海,唐辉,李倩,张胜梅,李晓霞,2016.香格里拉地区山脉大气微生物分布及药敏情况.解放军预防医学杂志,34(2):159-161
Barnett HL,Hunter BB,1977.Illustrated genera of imperfect fungi(third edition).Translated by Shen CY.Science Press,Beijing.1-240(in Chinese)
Beaumont F,Kauffman HF,Vandermark TH,Sluiter HJ,Devries K,1985.Volumetric aerobiological survey of conidial fungi in the North-East Netherlands.I.Seasonal patterns and the influence of meteorological variables.Allergy,40:173-180
Chen DM,Li JG,Su XR,Yang G,2006.Anti-mildew of military electronic equipment.Equipment Environmental Engineering,3(4):78-81,95(in Chinese)
de Leo F,Campanella G,Proverbio E,Urzi C,2013.Laboratory tests of fungal biocorrosion of unbonded lubricated post-tensioned tendons.Construction and Building Materials,49:821
Dong AR,Sun W,Wang TL,2008.Diversity of indoor airborne fungi in Harbin city.Journal of Northeast Forestry University,36(2):77-79(in Chinese)
Fang ZG,Huang C,Lou XQ,Li RZ,Yao WC,2017.Characteristics of airborne microbes in typical tourist city in Southeast China.China Environmental Science,37(8):2840-2847(in Chinese)
Fang ZG,Ouyang ZY,2009.Advance of airborne fungal community and the influencing factors in indoor and outdoor environments in urban ecosystem.Ecology and Environmental Sciences,18(1):386-393(in Chinese)
Garrett MH,Rayment PR,Hooper MA,Abramson MJ,Hooper BM,1998.Indoor airborne fungal spores,house dampness and associations with environmental factors and respiratory health in children.Clinical and Experimental Allergy,28(4):459-467
He XS,2015.Modern fungal classification system.Beijing Science Press,Beijing.1-627(in Chinese)
Hu LF,Fang ZG,Ouyang ZY,Liao XL,Lin XQ,Wang XK,2005.Ecological distributions of airborne fungi in outdoor environments in Beijing.Chinese Journal of Environmental Science,26(5):22-27(in Chinese)
Hu QX,Li JB,1994.Effect of solar radiation on the airborne fungi.Shanghai Environmental Sciences,13(2):19-21,32(in Chinese)
Huang JJ,Hong SL,Zhou W,Yang YC,2002.Air-borne molds in Chongqing city.Chongqing Medical Journal,31(8):701-703(in Chinese)
Ju TZ,Suo AM,Tian YJ,Feng KK,2003.Analysis on aerobiologia in Lanzhou.Industrial Safety and Environmental Protection,29(3):17-19
Kirk PM,Cannon PF,Minter DW,Stalpers JA,2008.Dictionary of the fungi.10th ed.CABI Publishing,Wallingford.1-784
Li CS,Kuo YM,1994.Characteristics of airborne microfungi in subtropical homes.Science of the Total Environment,155(3):267-271
Li DW,Kendrick B,1994.Functional relationships between airborne fungal spores and environmental factors in Kitchener-Waterloo,Ontario,as detect by canonical correspondence analysis.Grana,33:166-176
Lindmanm J,Constantinidou H,Barchet W,1982.Plants as sources of airborne bacteria,including ice nucleationaction bacteria.Grana,44(5):1059
Ling Q,Wang YP,Wang L,Shu Y,Tao Y,Bao LN,2008.Distribution of airborne fungi concentration in Hefei City,Anhui.Biodiversity Science,16(2):175-180(in Chinese)
Liu QQ,Lu L,Gao G,Li XG,2017.Research progress on fungal corrosion of metals and their protective layers in atmospheric environments.Journal of University of Science and Technology Beijing,39(10):1463-1469(in Chinese)
Liu SD,Kong WX,Wang J,2016.Investigation of typical marine mould corrosion environment-a case study of“DONG FANG HONG 2”.Guangzhou Chemical Industy,44(21):141-143(in Chinese)
Mouli C,Mohan S,Reddy S,2005.Assessment of microbial(bacteria)concentrations of ambient air at semi-arid urban region:influence of meteorological factors.Aeer,3:139-149
Nisiotou AA,Spriopoulos AE,Nychas GJ,2007.Yeast community structures and dynamics in healthy and bortytis-affected grape must fermentations.Applied and Environmental Microbiology,73(21):6705-6713
Ouyang YS,Chen YB,Xie XB,Huang XM,Chen JD,Peng H,Li JY,2003.Determination of airborne microbial cancentration around key traffic route in Guangzhou urbal districts.Chinses Journal of Health Laboratory Technology,13(6):692-693(in Chinese)
Peng WH,Bau T,2012.A review on community diversity of urban airborne fungi.Journal of Fungal Research,10(1):49-56(in Chinese)
Salvarezza RC,de Mele MFL,Videla HA,1983.Mechanisms of the microbial corrosion of aluminum alloys.Corrosion,39(1):26
Shao LP,Shen RX,Zhang SX,Xiang CT,Tan SS,1984.Fungal taxonomy.China Forestry Press,Beijing.1-370(in Chinese)
Song ZW,Wang L,Ren J,2014.Concentration distribution and characteristics of airborne microbes in spring and summer in Qingdao.Journal of Qingdao Technological University,35(3):1-6,23(in Chinese)
Tai FL,1987.Morphology and taxonomy of fungi.Science Press,Beijing.1-352(in Chinese)
Wei CT,1979.Fungal identification manual.Shanghai Science and Technology Press,Shanghai.1-780(in Chinese)
Wu P,Su H,Lin C,2000.Characteristics of indoor and outdoor airborne fungi at suburban and urban homes in two seasons.Science of the Total Environment,253:111-118
Zhang YL,Zheng SY,Zhi Q,Liu DB,2007.Detection of airborne fungus in different types of military underground construction.Journal of Environmental Occupational Medicine,24(4):454-455(in Chinese)
Zi JW,Zi YH,Tang H,Li Q,Zhang SM,Li XX,2015.Distribution and drug sensitivity of mountain air microorganisms at Deqin district in Yunnan Province.Medical Journal of National Defending Forces in Southwest China,25(10):1153-1156(in Chinese)
Zi JW,Zi YH,Tang H,Li Q,Zhang SM,Li XX,2016.Distribution and drug sensitivity of mountain air microorganisms at Shangri-La district in Yunnan Province.Journal of Preventive Medicine of Chinese People’s Liberation Army,34(2):159-161(in Chinese)
白雪,史应武,董秀黄,薛娟,李萍,娄恺,2013.塔克拉玛干沙漠西缘春季沙尘天气大气可培养真菌多样性.新疆农业科学,50(7):1314-1321
陈丹明,李金国,苏兴荣,杨光,2006.军用电子装备的防霉.装备环境工程,3(4):78-81,95
戴芳澜,1987.真菌的形态和分类.北京:科学出版社.1-352
董爱荣,孙巍,王天亮,2008.哈尔滨室内大气真菌多样性.东北林业大学学报,36(2):77-79
方治国,黄闯,楼秀芹,李睿哲,姚文冲,2017.南方典型旅游城市大气微生物特征研究.中国环境科学,37(8):2840-2847
方治国,欧阳志云,2009.城市室内外大气真菌群落及影响因素研究进展.生态环境学报,18(1):386-393
贺新生,2015.现代菌物分类系统.北京:科学出版社.1-627
H.L.巴尼特,B.B亨特(著),沈崇尧(译),1977.半知菌属图解(第三版).北京:科学出版社.1-240
胡利锋,方治国,欧阳志云,廖晓兰,林学强,王效科,2005.北京室外大气真菌分布特征.环境科学,26(5):22-27
胡庆轩,李军保,1994.日光辐射对大气真菌粒子的影响.上海环境科学,13(2):19-21,32
黄江菊,洪苏玲,周伟,杨玉成,2002.重庆城区大气中致敏真菌调查.重庆医学,31(8):701-703
凌琪,王晏平,王莉,舒莹,陶勇,鲍立宁,2008.合肥城区大气真菌浓度的时空分布特征.生物多样性,16(2):175-180
刘倩倩,卢琳,高歌,李晓刚,2017.大气环境中金属及其保护层霉菌腐蚀研究的进展.工程科学学报,39(10):1463-1469
刘士栋,孔维轩,王佳,2016.典型海洋霉菌腐蚀环境调查-“东方红2”为例.广州化工,44(21):141-143
欧阳友生,陈仪本,谢小保,黄小茉,陈娇娣,彭红,李剑英,2003.广州城区主要交通枢纽大气微生物浓度的测定.中国卫生检验杂志,13(6):692-693
彭炜航,图力古尔,2012.城市大气真菌群落多样性研究概况.菌物研究,10(1):49-56
邵力平,沈瑞祥,张素轩,项存悌,谭松山,1984.真菌分类学.北京:中国林业出版社.1-370
宋志文,王琳,任杰,2014.青岛市区春、夏季生物气溶胶浓度分布及特征.青岛理工大学学报,35(3):1-6,23
魏景超,1979.真菌鉴定手册.上海:上海科学技术出版社.1-780
张永良,郑世英,智强,刘东彪,2007.不同类型地下工事大气真菌检测分析.环境与职业医学,24(4):454-455
子建文,资云海,唐辉,李倩,张胜梅,李晓霞,2015.云南德钦地区山脉大气微生物分布及药敏情况.西南国防医药,25(10):1153-1156
子建文,资云海,唐辉,李倩,张胜梅,李晓霞,2016.香格里拉地区山脉大气微生物分布及药敏情况.解放军预防医学杂志,34(2):159-161