基于多元统计分析的茜草药材指纹图谱研究
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摘要
目的 建立茜草药材的高效液相指纹图谱,为不同产地茜草药材的质量评价提供方法。方法 采用高效液相谱法对10批不同产地茜草进行测定;采用相似度评价、聚类分析、主成分分析3种方法对指纹图谱进行研究。结果 建立了茜草HPLC指纹图谱共有模式图标定了12个共有峰,并指认了4个共有峰化学成分;10批茜草指纹图谱与对照指纹图谱之间相似度均大于0.90; 10批次样品被聚类为两大类;主成分分析显示,提取出5个主成分,累计方差贡献率达93.78%。结论 建立的指纹图谱较全面、直观地反映了茜草整体化学成分信息,结合多元统计分析,为茜草药材的质量评价提供参考。
Objective To establish a HPLC fingerprint method of Radix Rubiae and provide a comprehensive evaluation of the drug from different habitats. Methods The high-performance liquid chromatography method was used to measure 10 batches of Radix Rubiae in different producing areas and the fingerprints were studied by similarity evaluation, cluster analysis and principal component analysis.Results HPLC fingerprint of Radix Rubiae was established. 12 common peaks were designated and the chemical composition of 4 common peaks were identified;the similarity of 10 batches of Radix Rubiae was over 0.90; Five principal components were extracted and the cumulative contribution rate was 93.78%.Conclusion Combined with multivariate statistical analysis, the fingerprint analysis which comprehensively reflected most of the chemical information of Radix Rubiae provides a reference for the quality evaluation of Radix Rubiae.
Objective To establish a HPLC fingerprint method of Radix Rubiae and provide a comprehensive evaluation of the drug from different habitats. Methods The high-performance liquid chromatography method was used to measure 10 batches of Radix Rubiae in different producing areas and the fingerprints were studied by similarity evaluation, cluster analysis and principal component analysis.Results HPLC fingerprint of Radix Rubiae was established. 12 common peaks were designated and the chemical composition of 4 common peaks were identified;the similarity of 10 batches of Radix Rubiae was over 0.90; Five principal components were extracted and the cumulative contribution rate was 93.78%.Conclusion Combined with multivariate statistical analysis, the fingerprint analysis which comprehensively reflected most of the chemical information of Radix Rubiae provides a reference for the quality evaluation of Radix Rubiae.
引文
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[2] 魏·吴普等述.孙星衍,孙冯翼辑.神农本草经[M].北京:科学技术文献出版社,1996:28.
[3] 陈毅,王海丽,薛露,等.茜草的研究进展[J].中草药,2017,48(13):2771.
[4] 李俊峰,舒建昌,杨钦河,等.大叶茜草素抑制大鼠CFSC-2G细胞活化和胶原合成[J].中国病理生理杂志,2017,33(12):2259.
[5] Manoharan RK,Lee JH,Kim YG.Alizarin and chrysazin inhibit biofilm and hyphal formation by front cell infect microbiol[J].Fron Cell Infect Microbiol,2017,7:447.
[6] Zengin G,Degirmenci NS,Alpsoy L.Evaluation of antioxidant,enzyme inhibition,and cytotoxic activity of three anthraquinones (alizarin,purpurin,and quinizarin)[J].Hum Exp Toxicol,2016,35(5):544.
[7] Nam W,Kim SP,Nam SH,et al.Structure-antioxidative and anti-inflammatory activity relationships of purpurin and related anthraquinones in chemical and cell assays[J].Molecules ,2017,22(2):265.
[8] Gong XP,Sun YY,Chen W,et al.Anti-diarrheal and anti-inflammatory activities of aqueous extract of the aerial part of Rubia cordifolia[J].BMC Complement Altern Med,2017,17(1):20.
[9] 荀宁.茜草及其伪品的质量控制研究[D].北京中医药大学硕士学位论文,2014.
[10] 李鹏,胡正海.茜草的生物学及化学成分与生物活性研究进展[J].中草药,2013,44(14):2009.
[11] 尹江宁,高峻玉.茜草多糖的研究进展[J].中华中医药学刊,2007,25(1):128.
[12] 姚令文,刘燕,郑笑为,等.指纹图谱、特征图谱技术在中药材和中成药中的应用[J].中国新药杂志,2018,27(8):934.
[13] 管仁伟,王幼鹏,魏丕振,吕玲霞,辛立红,林慧彬,林建强.不同种质黄芩高效液相色谱指纹图谱研究[J].时珍国医国药,2018,29(5):1102.
[14] 姚东,孟宪生,王帅,等.气滞胃痛颗粒全时段多波长融合指纹图谱研究及多成分定量分析[J].中国中药杂志,2013,38(10):1513.