中海拔地区肺癌患者血浆多种血管相关细胞因子联合检测的研究
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摘要
目的通过检测中海拔地区肺癌患者及对照组血浆中VEGF、TGF-β1、TNF-α、Ang-2的表达水平,探讨对肺癌的诊断及临床价值。方法选取中海拔地区(2 260 m)病理确诊为NSCLC的患者和对照组各30例。ELISA检测血浆中VEGF、TGF-β1、TNF-α、Ang-2的表达水平。结果中海拔地区肺癌组血浆中VEGF、TGF-β1、TNF-α的水平高于对照组,差异有统计学意义(P <0.05)。单项检测对肺癌诊断的灵敏度及特异度分别为VEGF 86.36%、80%,TGF-β1 90.91%、76.67%,TNF-α86.36%、76.67%,联合检测的灵敏度及特异度为95.45%,70%。结论 VEGF、TGF-β1、TNF-α在NSCLC患者血清中升高,VEGF对NSCLC诊断具有有较好的灵敏度和特异性,而联合检测可以提高灵敏度的同时特异性也有所下降。
Objective The expression of VEGF,TGF-β1,TNF-α and Ang-2 in plasma of lung cancer patients and healthy controls were detected in middle altitude area. To explore the diagnosis and clinical value of lung cancer. Method Lung cancer group(n = 30)and healthy control group(n = 30)were selected. The expression level of VEGF,TGF-β1,TNF-α and Ang-2 in two groups of plasma was detected by ELISA. Results The plasma level of VEGF,TGF-β1,TNF-α in lung cancer group at middle altitude was higher than that in the control group(P < 0.05). The sensitivity and specificity of three cytokines in the plasma of lung cancer patients were 86.36% and80% of VEGF,90.91% and 76.67% of TGF-β1,86.36% and 76.67% of TNF-α. The sensitivity and specificity of the three cytokines for combined detection were 95.45% and 70%. Conclusion VEGF,TGF-β1,and TNF-α are elevated in the serum of patients with NSCLC. VEGF has a good sensitivity and specificity for the diagnosis of NSCLC.Combined detection can increase the sensitivity as well as decrease the specificity for the diagnosis of NSCLC.
Objective The expression of VEGF,TGF-β1,TNF-α and Ang-2 in plasma of lung cancer patients and healthy controls were detected in middle altitude area. To explore the diagnosis and clinical value of lung cancer. Method Lung cancer group(n = 30)and healthy control group(n = 30)were selected. The expression level of VEGF,TGF-β1,TNF-α and Ang-2 in two groups of plasma was detected by ELISA. Results The plasma level of VEGF,TGF-β1,TNF-α in lung cancer group at middle altitude was higher than that in the control group(P < 0.05). The sensitivity and specificity of three cytokines in the plasma of lung cancer patients were 86.36% and80% of VEGF,90.91% and 76.67% of TGF-β1,86.36% and 76.67% of TNF-α. The sensitivity and specificity of the three cytokines for combined detection were 95.45% and 70%. Conclusion VEGF,TGF-β1,and TNF-α are elevated in the serum of patients with NSCLC. VEGF has a good sensitivity and specificity for the diagnosis of NSCLC.Combined detection can increase the sensitivity as well as decrease the specificity for the diagnosis of NSCLC.
引文
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[3]YANG S,SUI J,LIANG G.Diagnosis value of aberrantly expressed microRNA profiles in lung squamous cell carcinoma:A study based on the Cancer Genome Atlas[J].PeerJ,2017,5:e4101.
[4]KUMAR H,CHOI D K.Hypoxia inducible factor pathway and physiological adaptation:A cell survival pathway?[J].Mediators Inflamm,2015,2015:584758.
[5]REINMUTH N,RECK M,GROHéC.Anti-angiogenic strategy:Therapy optimization of non-squamous non-small cell lung carcinoma[J].Pneumologie,2014,68(12):793-798.
[6]CHAKRABORTY S,ADHIKARY A,MAZUMDAR M,et al.Capsaicin-induced activation of p53-SMAR1 auto-regulatory loop down-regulates VEGF in non-small cell lung cancer to restrain angiogenesis[J].PLoS One,2014,9(6):e99743.
[7]CHA N,LV M,ZHAO Y J,et al.Diagnostic utility of VEGFmRNA and SP1 m RNA expression in bronchial cells of patients with lung cancer[J].Respirology,2014,19(4):544-548.
[8]PENG J,AN S,WANG H P,et al.Video-assisted thoracoscopic surgery lobectomy for lung cancer versus thoracotomy:A less decrease in sVEGFR2 level after surgery[J].J Thorac Dis,2016,8(3):323-328.
[9]SZYSZKA-BARTH K,RAMLAU K,STENCEL D,et al.Antiangiogenic agents in the treatment of non-small cell lung cancer[J].Kardiochir Torakochirurgia Pol,2014,11(2):145-150.
[10]YANG S,CHO Y J,JIN L,et al.An epigenetic auto-feedback loop regulates TGF-βtype II receptor expression and function in NSCLC[J].Oncotarget,2015,6(32):33237-33252.
[11]RISOLINO M,MANDIA N,IAVARONE F,et al.Transcription factor PREP1 induces EMT and metastasis by controlling the TGF-β-SMAD3 pathway in non-small cell lung adenocarcinoma[J].Proc Natl Acad Sci U S A,2014,111(36):E3775-84.
[12]REN Y,YIN Z,LI K,et al.TGFβ-1 and TGFBR2 polymorphisms,cooking oil fume exposure and risk of lung adenocarcinoma in Chinese nonsmoking females:A case control study[J].BMC Med Genet,2015,16:22.
[13]WANG H,ZHAO Y P,GAO C F,et al.Transforming growth factor beta 1 gene variants increase transcription and are associated with liver cirrhosis in Chinese[J].Cytokine,2008,43(1):20-25.
[14]LU X,GUO H,CHEN X,et al.Effect of RhoC on the epithelial-mesenchymal transition process induced by TGF-β1 in lung adenocarcinoma cells[J].Oncol Rep,2016,36(6):3105-3112.
[15]PARK J S,KIM I K,HAN S,et al.Normalization of tumor vessels by Tie2 activation and Ang2 inhibition enhances drug delivery and produces a favorable tumor microenvironment[J].Cancer Cell,2016,30(6):953-967.
[16]YU Q,STAMENKOVIC I.Angiopoietin-2 is implicated in the regulation of tumor angiogenesis[J].Am J Pathol,2001,158(2):563-570.
[17]LIU X,YU X,XIE J,et al.ANGPTL2/LILRB2 signaling promotes the propagation of lung cancer cells[J].Oncotarget,2015,6(25):21004-21015.