不同采收期半枝莲中黄酮含量比较
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摘要
目的以黄酮类含量为指标,确定半枝莲药材的最佳采收期。方法以野黄芩苷为对照品,采用紫外-可见分光光度法测定不同采收期半枝莲中总黄酮含量,采用高效液相色谱(HPLC)法测定其中野黄芩苷及大波斯菊的含量。结果不同采收期的半枝莲中总黄酮和野黄芩苷的含量均达到了《中华人民共和国药典》的规定标准,在开花期半枝莲的总黄酮和野黄芩苷的含量最高,其中总黄酮的平均含量(45.74±0.95)mg·g-1,野黄芩苷的平均含量(5.58±0.16)mg·g-1,大波斯菊苷的平均含量(17.39±0.42)mg·g-1。结论半枝莲药材的最佳采收期应为枝繁叶茂的开花期。
Objective To determine the optimum harvest time of Scutellaria barbata D.Don with flavonoids contents as the index.Methods The content of total flavonoids in Scutellaria barbata D.Don was determined by ultraviolet-visible spectrophotometry with scutellarin as standard control,and scutellarin and cosmosiin in Scutellaria barbata D.Don were determined by HPLC.Results The contents of total flavonoids and scutellarin in Scutellaria barbata D.Don of different harvest time were complied with the quality standards in the China Pharma Copoeia,and reached the peakin blossom.The contents of total flavonoids,scutellarin and cosmosiin were(45.74±0.95),(5.58 ± 0.16) and(17.39 ± 0.42) mg·g-1,respectively.Conclusion The Scutellaria Barbata D.Don may be collected at the flowering time with luxuriant foliage.
Objective To determine the optimum harvest time of Scutellaria barbata D.Don with flavonoids contents as the index.Methods The content of total flavonoids in Scutellaria barbata D.Don was determined by ultraviolet-visible spectrophotometry with scutellarin as standard control,and scutellarin and cosmosiin in Scutellaria barbata D.Don were determined by HPLC.Results The contents of total flavonoids and scutellarin in Scutellaria barbata D.Don of different harvest time were complied with the quality standards in the China Pharma Copoeia,and reached the peakin blossom.The contents of total flavonoids,scutellarin and cosmosiin were(45.74±0.95),(5.58 ± 0.16) and(17.39 ± 0.42) mg·g-1,respectively.Conclusion The Scutellaria Barbata D.Don may be collected at the flowering time with luxuriant foliage.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010:109-110.
[2]仲浩,薛晓霞,姚庆强.半枝莲化学成分的研究[J].中草药,2008,39(1):21-23.
[3]杨培芬,李钧敏,邵红.半枝莲的次生代谢产物含量测定与体外抑菌活性的研究[J].四川中医,2005,23(11):35-36.
[4]曾莉萍,林明宝,舒虹.复方半枝莲胶囊体外抗肿瘤作用的实验研究[J].江西医学院学报,2006,46(3):46-48.
[5]郭珊珊,时宇静,高英杰,等.半枝莲总黄酮抗副流感病毒的作用机制[J].药学学报,2009,44(12):1348-1352.
[6]冯德富,李小沙.复方半枝莲汤联合阿德福韦酯治疗慢性乙型肝炎疗效观察[J].实用中医内科杂志,2010,24(2):72-75.
[7]石扬,周志愉,程流剑,等.半枝莲中野黄芩苷对乳腺癌MCF-7细胞增殖及侵袭能力的影响[J].江西医学院学报,2009,49(11):12-14.
[8]范悦,吴晓光,缪红,等.半枝莲黄酮对β-淀粉样蛋白所致星形胶质细胞损伤的影响[J].医药导报,2015,34(2):141-145.
[9]李洁.HPLC测定半枝莲不同药用部位野黄芩苷的含量[J].辽宁中医杂志,2008,35(3):427-428.
[2]仲浩,薛晓霞,姚庆强.半枝莲化学成分的研究[J].中草药,2008,39(1):21-23.
[3]杨培芬,李钧敏,邵红.半枝莲的次生代谢产物含量测定与体外抑菌活性的研究[J].四川中医,2005,23(11):35-36.
[4]曾莉萍,林明宝,舒虹.复方半枝莲胶囊体外抗肿瘤作用的实验研究[J].江西医学院学报,2006,46(3):46-48.
[5]郭珊珊,时宇静,高英杰,等.半枝莲总黄酮抗副流感病毒的作用机制[J].药学学报,2009,44(12):1348-1352.
[6]冯德富,李小沙.复方半枝莲汤联合阿德福韦酯治疗慢性乙型肝炎疗效观察[J].实用中医内科杂志,2010,24(2):72-75.
[7]石扬,周志愉,程流剑,等.半枝莲中野黄芩苷对乳腺癌MCF-7细胞增殖及侵袭能力的影响[J].江西医学院学报,2009,49(11):12-14.
[8]范悦,吴晓光,缪红,等.半枝莲黄酮对β-淀粉样蛋白所致星形胶质细胞损伤的影响[J].医药导报,2015,34(2):141-145.
[9]李洁.HPLC测定半枝莲不同药用部位野黄芩苷的含量[J].辽宁中医杂志,2008,35(3):427-428.