半枝莲不同采收期质量综合评价
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摘要
[目的]以多成分含量为指标,掌握半枝莲不同采收期质量变化规律,确定半枝莲药材的最佳采收期。[方法]采用紫外-可见分光光度法测定不同采收期半枝莲中总黄酮含量,高效液相色谱法同时测定野黄芩苷、野黄芩素、木犀草素、芹菜素等多成分的含量,并以水溶性、醇溶性浸出物为评价指标。[结果]不同采收期总黄酮、野黄芩苷的含量从大到小依次为7月、9月、5月,不同生长期含量从大到小依次为开花期、开花前、果实期。其他多成分指标中,野黄芩苷及浸出物含量均以7月开花期采收的半枝莲最高,而木犀草素、芹菜素含量则表现不同的规律。[结论]不同采收期半枝莲中质量差异明显,建议应以7月开花期为半枝莲最佳采收期。
[Objective] The research aimed to grasp different harvesting periods quality of the law of change and determine the optimum harvest time of Scutellaria barbata with multi-component content as the index. [Methods]The content of total flavonoids in different harvesting periods of Scutellaria barbata was determined by UV spectrophotometry,and the content of scutellarin,scutellarein,luteolin,apigenin were simultaneously determined by HPLC,and with water soluble extract,alcohol soluble extract as the evaluation index. [Result]The contents of total flavonoids in different harvesting periods were in the order of July> September> May,the content in different growth stages were flowering period > pre-flowering> fruit stage. In other multi-component indexes,the content of Scutellaria baicalensis and water soluble extract,alcohol soluble extract were the highest in flowering in July,while the content of luteolin and apigenin showed different rules. [Conclusion]Scutellaria barbata in different harvesting periods have significant differences in quality and should be collected in flowering period in July.
[Objective] The research aimed to grasp different harvesting periods quality of the law of change and determine the optimum harvest time of Scutellaria barbata with multi-component content as the index. [Methods]The content of total flavonoids in different harvesting periods of Scutellaria barbata was determined by UV spectrophotometry,and the content of scutellarin,scutellarein,luteolin,apigenin were simultaneously determined by HPLC,and with water soluble extract,alcohol soluble extract as the evaluation index. [Result]The contents of total flavonoids in different harvesting periods were in the order of July> September> May,the content in different growth stages were flowering period > pre-flowering> fruit stage. In other multi-component indexes,the content of Scutellaria baicalensis and water soluble extract,alcohol soluble extract were the highest in flowering in July,while the content of luteolin and apigenin showed different rules. [Conclusion]Scutellaria barbata in different harvesting periods have significant differences in quality and should be collected in flowering period in July.
引文
[1]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015:118.
[2]郭姗姗,时宇静,高英杰,等.半枝莲总黄酮抗副流感病毒的作用机制[J].药学学报,2009,44(12):1348-1352.
[3]曾莉萍,林明宝,舒虹.复方半枝莲胶囊体外抗肿瘤作用的实验研究[J].江西医学院学报,2006,46(3):46-48.
[4]杨培芬,李钧敏,邵红.半枝莲的次生代谢产物含量测定与体外抑菌活性的研究[J].四川中医,2005,23(11):35-36.
[5]冯德富,李小沙.复方半枝莲汤联合阿德福韦酯治疗慢性乙型肝炎疗效观察[J].实用中医内科杂志,2010,24(2):72-75.
[6]南京中医药大学.中药大辞典·上册[M].2版.上海:上海科学技术出版社,2006:1079.
[7]国家中医药管理局《中华本草》编委会.中华本草7[M].上海:上海科学技术出版社,1999:210-212.
[8]邹箴蕾,吴启南.HPLC测定不同采收时间半枝莲中的野黄芩苷含量[J].现代中药研究与实践,2005,19(2):45-46.
[9]李洁.不同采收期半枝莲中总黄酮含量的比较研究[J].中医药导报,2008,14(5):99,112.
[10]范菊娣,覃容贵,李相陵,等.不同采收期半枝莲中黄酮含量比较[J].医药导报,2016,35(9):987-990.
[2]郭姗姗,时宇静,高英杰,等.半枝莲总黄酮抗副流感病毒的作用机制[J].药学学报,2009,44(12):1348-1352.
[3]曾莉萍,林明宝,舒虹.复方半枝莲胶囊体外抗肿瘤作用的实验研究[J].江西医学院学报,2006,46(3):46-48.
[4]杨培芬,李钧敏,邵红.半枝莲的次生代谢产物含量测定与体外抑菌活性的研究[J].四川中医,2005,23(11):35-36.
[5]冯德富,李小沙.复方半枝莲汤联合阿德福韦酯治疗慢性乙型肝炎疗效观察[J].实用中医内科杂志,2010,24(2):72-75.
[6]南京中医药大学.中药大辞典·上册[M].2版.上海:上海科学技术出版社,2006:1079.
[7]国家中医药管理局《中华本草》编委会.中华本草7[M].上海:上海科学技术出版社,1999:210-212.
[8]邹箴蕾,吴启南.HPLC测定不同采收时间半枝莲中的野黄芩苷含量[J].现代中药研究与实践,2005,19(2):45-46.
[9]李洁.不同采收期半枝莲中总黄酮含量的比较研究[J].中医药导报,2008,14(5):99,112.
[10]范菊娣,覃容贵,李相陵,等.不同采收期半枝莲中黄酮含量比较[J].医药导报,2016,35(9):987-990.