一种具有潜在解酒护肝功能酵素的开发及其生物活性评价
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摘要
文章以明确报道的具有解酒护肝功效的葛根、葛花、丹参等药食同源材料为原料,经复合酶酶解和鼠李糖乳杆菌217-8发酵制备成酵素,对其黄酮、葛根素、多酚等活性成分含量及总抗氧化能力进行检测。结果表明,该酵素与原材料对照,其葛根素含量提高了7倍,黄酮含量提高了3倍,氨基酸含量提高了3倍,多酚含量提高了80 mg/L,抗氧化能力提高了92%,以上指标均是与加速酒精代谢及保护肝脏相关的指标。因此,优选并配伍的葛根、葛花、丹参、山楂、云茯苓等药食同源原料经复合酶解和微生物转化制备成酵素,可将其天然活性成分完整保留并有效释放,同时产生有益活性次级代谢产物,具有潜在的解酒护肝功能。
This article was based on well-reported drug and food homogenous materials, such as Pueraria lobata, Flos puerariae and Salvia mil, which have antialcoholism and liver protection effects. The ferment was prepared by enzymatic hydrolysis of complex enzymes and the fermentation of Lactobacillus rhamnosus 217-8. The content of active ingredients such as flavonoids, puerarin, polyphenols and total antioxidant capacity were tested. The results showed that the content of puerarin was increased 7-fold, the content of flavonoids was increased 3 times, the content of amino acids was increased 3 times, the content of polyphenols was increased by 80 mg/L, and compared with raw materials, the antioxidant capacity was improved 92%. The above indicators were related to accelerating alcohol metabolism and protecting the liver. Therefore, preferred and compatible raw materials of Pueraria lobata, Flos puerariaeand Salvia mil, hawthorn, and poria cocos, etc, are prepared into ferment by complex enzymolysis and microbial transformation, and their natural active ingredients can be completely preserved and effectively released. At the same time, beneficial secondary metabolites were generated, which have potential antialcoholism and liver protection functions.
This article was based on well-reported drug and food homogenous materials, such as Pueraria lobata, Flos puerariae and Salvia mil, which have antialcoholism and liver protection effects. The ferment was prepared by enzymatic hydrolysis of complex enzymes and the fermentation of Lactobacillus rhamnosus 217-8. The content of active ingredients such as flavonoids, puerarin, polyphenols and total antioxidant capacity were tested. The results showed that the content of puerarin was increased 7-fold, the content of flavonoids was increased 3 times, the content of amino acids was increased 3 times, the content of polyphenols was increased by 80 mg/L, and compared with raw materials, the antioxidant capacity was improved 92%. The above indicators were related to accelerating alcohol metabolism and protecting the liver. Therefore, preferred and compatible raw materials of Pueraria lobata, Flos puerariaeand Salvia mil, hawthorn, and poria cocos, etc, are prepared into ferment by complex enzymolysis and microbial transformation, and their natural active ingredients can be completely preserved and effectively released. At the same time, beneficial secondary metabolites were generated, which have potential antialcoholism and liver protection functions.
引文
[1]王辉,王江滨.肝炎病毒感染与酒精性肝硬化关系的研究[J].吉林大学学报(医学版),1998,(6):652-653
[2] O'Shea R S, Dasarathy S, Mccullough A J. Alcoholic liver disease[J]. Lancet,1995,51(1):227-229
[3] Ferenci P,Dragosics B, Dittrich H,et al. Randomized controlled trial of silymarin treatment in patients with cirrhosis of the liver[J]. Journal of Hepatology,1989,9(1):105-113
[4]单峰,黄璐琦,郭娟,等.药食同源的历史和发展概况[J].生命科学,2015,27(8):1061-1069
[5] Arango V, Underwood M D, Mann J J. Fewer pigmented neurons in the locus coeruleus of uncomplicated alcoholics[J]. Brain Research, 1994,650(1):1-8
[6]张镛.酒精中毒与神经损害[J].卒中与神经疾病,1994,(4):112-114
[7]中岛嘉次郎.葛花对消化道的影响[J].国际中医中药杂志,2002,24(4):250-250
[8] Lin R C, Li T K. Effects of isoflavones on alcohol pharmacokinetics and alcohol-drinking behavior in rats[J]. American Journal of Clinical Nutrition, 1998,68(6Suppl):1512S-1515S
[9] Lee M K,Cho S Y, Jang J Y,et al. Effects of Puerariae Flos and Puerariae Radix extracts on antioxidant enzymes in ethanol-treated rats[J]. American Journal of ChineseMedicine,2001,29(02):343-354
[10]薛惠明,胡义扬,顾宏图,等.丹参抗CCl4及DMN诱导的大鼠肝纤维化的研究[J].中西医结合肝病杂志,1999,(4):16-18
[11]王昂,王丽丽,仪宏,等.茚三酮比色法测定谷氨酸含量的研究[J].中国调味品,2005,(8):50-52
[12] Hodisan T, Culea M, Cimpoiu C, et al. Separation,identification and quantitative determination of free amino acids from plant extracts[J]. Journal of Pharmaceutical&Biomedical Analysis, 1998,18(3):319-323
[13]李静,轰继云,李海飞,等.Folin-酚法测定水果及其制品中总多酚含量的条件[J].果树学报,2008,25(1):126-131
[14]陈芳甜.观赏海棠果抗氧化活性的研究[D].泰安:山东农业大学,2012
[15]韩墨,王燕,杨志鹏,等.内蒙古传统酸乳乳酸菌的筛选及体外益生效果评价[J].食品研究与开发,2018,(1):152-156
[16] TIAN F, ZHAI Q, ZHAO J, et al. Lactobacillus plantarum CCFM8661 alleviates lead toxicity in mice[J].Biological trace element research,2012,150:264-271
[17]高学清.葛根和葛花的解酒护肝作用及其机制研究[D].无锡:江南大学,2013
[18]孙冀平,沈蓓英.蛋白肽饮料的醒酒机制和制备工艺的探讨[J].食品与发酵工业,1999,25(4):64-66
[19]赵鹏,姚思宇,李凤文,等.葛根黄酮对乙醇性肝损伤的保护作用[J].中国热带医学,2009,9(3):444-445
[20] Halliwell B, Gutteridge J M C. Free radicals in biology and medicine[J]. Journal of Free Radicals in Biology&Medicine,2007,4(1):331-332
[2] O'Shea R S, Dasarathy S, Mccullough A J. Alcoholic liver disease[J]. Lancet,1995,51(1):227-229
[3] Ferenci P,Dragosics B, Dittrich H,et al. Randomized controlled trial of silymarin treatment in patients with cirrhosis of the liver[J]. Journal of Hepatology,1989,9(1):105-113
[4]单峰,黄璐琦,郭娟,等.药食同源的历史和发展概况[J].生命科学,2015,27(8):1061-1069
[5] Arango V, Underwood M D, Mann J J. Fewer pigmented neurons in the locus coeruleus of uncomplicated alcoholics[J]. Brain Research, 1994,650(1):1-8
[6]张镛.酒精中毒与神经损害[J].卒中与神经疾病,1994,(4):112-114
[7]中岛嘉次郎.葛花对消化道的影响[J].国际中医中药杂志,2002,24(4):250-250
[8] Lin R C, Li T K. Effects of isoflavones on alcohol pharmacokinetics and alcohol-drinking behavior in rats[J]. American Journal of Clinical Nutrition, 1998,68(6Suppl):1512S-1515S
[9] Lee M K,Cho S Y, Jang J Y,et al. Effects of Puerariae Flos and Puerariae Radix extracts on antioxidant enzymes in ethanol-treated rats[J]. American Journal of ChineseMedicine,2001,29(02):343-354
[10]薛惠明,胡义扬,顾宏图,等.丹参抗CCl4及DMN诱导的大鼠肝纤维化的研究[J].中西医结合肝病杂志,1999,(4):16-18
[11]王昂,王丽丽,仪宏,等.茚三酮比色法测定谷氨酸含量的研究[J].中国调味品,2005,(8):50-52
[12] Hodisan T, Culea M, Cimpoiu C, et al. Separation,identification and quantitative determination of free amino acids from plant extracts[J]. Journal of Pharmaceutical&Biomedical Analysis, 1998,18(3):319-323
[13]李静,轰继云,李海飞,等.Folin-酚法测定水果及其制品中总多酚含量的条件[J].果树学报,2008,25(1):126-131
[14]陈芳甜.观赏海棠果抗氧化活性的研究[D].泰安:山东农业大学,2012
[15]韩墨,王燕,杨志鹏,等.内蒙古传统酸乳乳酸菌的筛选及体外益生效果评价[J].食品研究与开发,2018,(1):152-156
[16] TIAN F, ZHAI Q, ZHAO J, et al. Lactobacillus plantarum CCFM8661 alleviates lead toxicity in mice[J].Biological trace element research,2012,150:264-271
[17]高学清.葛根和葛花的解酒护肝作用及其机制研究[D].无锡:江南大学,2013
[18]孙冀平,沈蓓英.蛋白肽饮料的醒酒机制和制备工艺的探讨[J].食品与发酵工业,1999,25(4):64-66
[19]赵鹏,姚思宇,李凤文,等.葛根黄酮对乙醇性肝损伤的保护作用[J].中国热带医学,2009,9(3):444-445
[20] Halliwell B, Gutteridge J M C. Free radicals in biology and medicine[J]. Journal of Free Radicals in Biology&Medicine,2007,4(1):331-332