HPLC法同时检测刺五加叶中原儿茶酸、绿原酸、刺五加苷E、金丝桃苷及槲皮苷的含量
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摘要
建立HPLC同时定量刺五加叶中原儿茶酸、绿原酸、刺五加苷E、金丝桃苷及槲皮苷五种主要成分的方法。方法:色谱柱:Agilent Extend C18(4.6mm×25mm,5μm),柱温:30℃;流速1 mL/min;进样量:10μL;流动相A:0.3%磷酸-水溶液,流动相B:0.3%磷酸-乙腈;梯度洗脱,洗脱条件:0～3 min,95%A;3～10 min,95%～80%A;10～35 min,80%～70%A;35～40 min,70%～10%A;40～50 min,10%A;紫外检测波长:265 nm。结果:金丝桃苷和槲皮苷在20～240μg/mL,原儿茶酸、绿原酸、刺五加苷E分别在5～60μg/mL、167.2～2010μg/mL及6～72μg/mL下与峰面积呈良好的线性关系;平均加样回收率在99.6%～103.1%,RSD为1.5%～2.7%。结论:本方法简便可靠,适用于刺五加叶中的多成分含量测定。
The paper presents simultaneous determination Of protocatechuis acid, chlorogenic acid, acanthopanax E, hyperoside and quercetin from acanthopanax senticosus leaves by HPLC method as follow. Column method: Agilent Extend C18( 4.6 mmx25 mm, 5μm);Column temperature: 30℃; Flow Rate: 1 mL/min; Injection; 10μL; Mobil phase A: 0.3% phosphoric acid solution; Mobil phase B: 0.3% phosphoric acid-acetonitrile; Gradient elution condition: 0-3 min, 95%A; 3-10 min,95-80%A; 10-35 min, 80-70%A; 35-40 min,70-10%; 40-50 min, 10%A; UV detection wavelength;265 nm; Result: hyperoside and quercetin contents are 20-240μg/mL;protocatechuis acid, chlorogenic acid and acanthopanax E are 5-60μg/ml, 167.2-2010μg/mL and 6-72μg/mL respectively. There are a good liear relationship with the peak area at 72 μg/mL, the average recovery is 99.6-103.1% and the RSD is 1.5-2.7%.Conclusion: This method is simple and reliable, and is suitiable for the determination ofmulti-component in Acathopanax senticosus leaves
The paper presents simultaneous determination Of protocatechuis acid, chlorogenic acid, acanthopanax E, hyperoside and quercetin from acanthopanax senticosus leaves by HPLC method as follow. Column method: Agilent Extend C18( 4.6 mmx25 mm, 5μm);Column temperature: 30℃; Flow Rate: 1 mL/min; Injection; 10μL; Mobil phase A: 0.3% phosphoric acid solution; Mobil phase B: 0.3% phosphoric acid-acetonitrile; Gradient elution condition: 0-3 min, 95%A; 3-10 min,95-80%A; 10-35 min, 80-70%A; 35-40 min,70-10%; 40-50 min, 10%A; UV detection wavelength;265 nm; Result: hyperoside and quercetin contents are 20-240μg/mL;protocatechuis acid, chlorogenic acid and acanthopanax E are 5-60μg/ml, 167.2-2010μg/mL and 6-72μg/mL respectively. There are a good liear relationship with the peak area at 72 μg/mL, the average recovery is 99.6-103.1% and the RSD is 1.5-2.7%.Conclusion: This method is simple and reliable, and is suitiable for the determination ofmulti-component in Acathopanax senticosus leaves
引文
[1]董文婷,霍金海,张海燕,等.刺五加叶的药理作用研究发展[J].中国实验方剂学杂志,2015,21(23):23-28.
[2]吴笛,张勉,张朝凤,等.款冬花中黄酮和酚酸类成分的研究[J].中国中药杂志,2010,35( 9 ):1142.
[3]罗旭,唐小龙,杨宋琪,等.不同产地刺五加药材高效液相色谱指纹图谱研究[J].中国药业,2017,26(20):66-68.
[2]吴笛,张勉,张朝凤,等.款冬花中黄酮和酚酸类成分的研究[J].中国中药杂志,2010,35( 9 ):1142.
[3]罗旭,唐小龙,杨宋琪,等.不同产地刺五加药材高效液相色谱指纹图谱研究[J].中国药业,2017,26(20):66-68.