脯氨酸引发提高烟草种子和幼苗抗逆性及其与抗氧化系统的关系
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摘要
以烟草种子为试验材料,以100 mmol/L浓度的脯氨酸(Pro)进行引发处理,测定干旱、低温、干旱+低温交叉胁迫下烟草种子和幼苗中APX和GPX的活性以及MDA,AsA和GSH的含量,研究Pro引发对烟草种子和幼苗的抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(GPX)活性以及丙二醛(MDA)、抗坏血酸(AsA)和谷胱甘肽(GSH)含量及其代谢相关酶活性的影响,探讨抗氧化系统与烟草逆境胁迫响应的关系,为烟草抗旱和抗寒研究提供理论依据。结果表明,在干旱、低温和干旱+低温交叉胁迫下,烟草幼苗抗氧化酶活性、抗氧化剂含量,以及还原型抗氧剂在总抗氧剂中所占的比值都普遍降低,但经Pro引发后的幼苗APX,GPX活性增强,MDA含量降低,AsA/(AsA+DHA)和GSH/(GSH+GSSH)的值增加。Pro引发提高了烟草幼苗的抗氧化能力,减缓和降低了由干旱和低温引起的氧化胁迫及其伤害,最终提高了烟草种子及幼苗的抗旱性和抗寒性。
The paper studied the influence on activity of ascorbate peroxidase(APX)and guaiacol peroxidase(GPX)and content of malondialdehyde(MDA),ascorbic acid(AsA)and glutathione(GSH)of tobacco seeds and seedlings by seed priming with proline,and explored the relationship between antioxidant systems and tobacco stress response,thus provided a theoretical basis for the drought resistance and cold resistance of tobacco research.Using 100 mmol/L proline to priming tobacco seeds,seeds priming and seedlings APX,GPX activity and MDA,ASA and GSH content was measured.The result showed that under the stress of drought,low temperature and drought + low temperature stress cross,antioxidant enzyme activity,antioxidant content and the ratio of ASA/(ASA+DHA)and GSH/(GSH+GSSH)of tobacco seedlings were generally decreased.However,APX,GPX activity increased,MDA content decreased,and the ratio of ASA/(ASA+DHA)and GSH/(GSH+GSSH)increased by proline priming.Proline priming can improve tobacco seedlings antioxidant capacity,slow and reduce the damage caused by drought and low temperature-induced oxidative stress,and ultimately improve tobacco's drought and cold resistance.
The paper studied the influence on activity of ascorbate peroxidase(APX)and guaiacol peroxidase(GPX)and content of malondialdehyde(MDA),ascorbic acid(AsA)and glutathione(GSH)of tobacco seeds and seedlings by seed priming with proline,and explored the relationship between antioxidant systems and tobacco stress response,thus provided a theoretical basis for the drought resistance and cold resistance of tobacco research.Using 100 mmol/L proline to priming tobacco seeds,seeds priming and seedlings APX,GPX activity and MDA,ASA and GSH content was measured.The result showed that under the stress of drought,low temperature and drought + low temperature stress cross,antioxidant enzyme activity,antioxidant content and the ratio of ASA/(ASA+DHA)and GSH/(GSH+GSSH)of tobacco seedlings were generally decreased.However,APX,GPX activity increased,MDA content decreased,and the ratio of ASA/(ASA+DHA)and GSH/(GSH+GSSH)increased by proline priming.Proline priming can improve tobacco seedlings antioxidant capacity,slow and reduce the damage caused by drought and low temperature-induced oxidative stress,and ultimately improve tobacco's drought and cold resistance.
引文
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[21]MALGORZATA MPOSMYK,CHRISTOPH BAILLY,KATARZY-NA SZAFRANSKA,et al.Antioxidant enzymes and Isoflavonoids in chilled soybean(Glycine max(L.)Merr.)seedlings[J].Journal of Plant Physiology,2005,162(4):403-412.
[2]彭珂珊,徐宣武,胡普辉,等.干旱是西部地区生态系统受损的关键因素[J].石家庄经济学院学报,2002,25(3):257-261.
[3]杜秀敏,殷文璇,赵彦修,等.植物中活性氧的产生及清除机制[J].生物工程学报,2001,17(2):121-215.
[4]刘紫娟,李萍,宗毓铮,等.干旱胁迫对糜子生理及产量的影响[J].山西农业科学,2016,44(9):1279-1283.
[5]钟连香,严理,秦武明,等.干旱胁迫对木荷幼苗生长及生理特性的影响[J].山西农业科学,2016,44(8):1103-1107.
[6]韩娅楠,米国全,史艳艳,等.低温对野生番茄和栽培番茄叶片抗氧化代谢和碳同化的影响[J].华北农学报,2018,33(3):153-158.
[7]蔺亚平,林海荣,崔辉梅.外源H2S和H2O2对Na Cl胁迫下加工番茄幼苗生理特性的影响[J].华北农学报,2018,33(3):159-166.
[8]梁艳荣,胡晓红,张颖力,等.植物过氧化物酶生理功能研究进展[J].内蒙古农业大学学报,2003,24(2):110-113.
[9]ASHRAF M,FOOLAD M R.Roles of glycine betaine and proline in improving plant a biotic stress resistance[J].Environ Exp Bot,2007,59:206-216.
[10]MOLINARI H B C,MARUR C J,FILHO J C B,et al.Osmotic adjustment in transgenic citrus rootstock Carrizo citrange(Citrus sinensis Osb.×Poncirus trifoliate L.Raf.)overproducing proline[J].Plant Sci,2004,167(6):1375-1381.
[11]YANG S L,LAN S S,GONG M.Hydrogen peroxide-induced proline and metabolic pathway of its accumulation in maize seedlings[J].Journal of Plant Physiology,2009(15):1694-1699.
[12]赵九洲,刘绍洪.渗透调节机制与植物的抗旱性研究[J].江西林业科技,2005(4):27-29.
[13]李忠光,杜朝昆.在单一提取系统中同时测定植物ASA/DSH和GSH/GSSG[J].云南师范大学学报,2003,23(3):67-70.
[14]CHANCE B,MAEHLY A C.Assay of catalase and peroxidase[J].Methods Enzymology,1955,2:764-775.
[15]李忠光,李江,杜朝昆,等.在单一提取系统中同时测定五种植物抗氧化酶[J].云南师范大学学报,2002,22(6):45-48.
[16]张志良.植物生理学实验指导[M].北京:高教出版社,2006.
[17]JIANG M Y,ZHANG J H.Effect of abscisic acid on active oxygen species,antioxidative defence system and oxidative damage in leaves of maize seedlings[J].Plant and Cell Physiology,2001,42(11):1265-1273.
[18]NAGALAKSHIMI N,PRASAD M N V.Responses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus[J].Plant Science,2001,160(2):291-299.
[19]NAVROT N,ROUHIER N,GELHAYE E,et al.Reactive oxygen species generation and antioxidant systems in plant mitochondria[J].Physiologia Plantarum,2007,129:185-195.
[20]LNZE D,MONTAGU M V.Oxidative stress in plants[J].Current Opinion Biotechnology,1995,6(2):153-158.
[21]MALGORZATA MPOSMYK,CHRISTOPH BAILLY,KATARZY-NA SZAFRANSKA,et al.Antioxidant enzymes and Isoflavonoids in chilled soybean(Glycine max(L.)Merr.)seedlings[J].Journal of Plant Physiology,2005,162(4):403-412.