扶正利湿抗癌方含药血清对前列腺癌PC-3细胞增殖的影响及机制研究
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摘要
目的:探讨扶正利湿抗癌方含药血清对前列腺癌细胞株PC-3体外增殖能力的影响及其作用机制。方法:应用CCK-8法测定不同浓度扶正利湿抗癌方含药血清对PC-3细胞生长抑制情况,流式细胞仪检测其对PC-3细胞凋亡及细胞周期的影响,并以Western blot分析扶正利湿抗癌方含药血清对PC-3细胞凋亡调控蛋白bcl-2和bax蛋白表达的影响。结果:扶正利湿抗癌方含药血清对PC-3细胞体外增殖抑制作用明显,呈浓度及时间依赖性。流式结果显示,扶正利湿抗癌方含药血清可诱导PC-3细胞凋亡,并能够增加G_2/M期细胞比例,降低S期细胞比例,呈浓度依赖性。Western blot结果显示,扶正利湿抗癌方含药血清可下调bcl-2蛋白的表达,而上调bax蛋白的表达,呈浓度依赖性。结论:扶正利湿抗癌方含药血清可以通过调节bcl-2和bax蛋白的表达发挥体外抑制PC-3细胞增殖的作用。
Objective: To explore the effects of Fuzheng Lishi Tang on the proliferatio of prostate cancer PC-3 cell lines and exploring the mechanism. Methods: The inhibitory effects of Fuzheng Lishi Tang on growth of PC-3 cells were detected by using CCK-8 assay. The influence of Fuzheng Lishi Tang on apoptosis and cell cycle of PC-3 cells was detected by flow cytometer. The influence of Fuzheng Lishi Tang on protein expressions of bcl-2 and bax of PC-3 cells was analyzed by Western blotting method. Results: The Fuzheng Lishi Tang had inhibitory effect on proliferation of PC-3 cells, its effective time and dose were positively correlated to cellular proliferation inhibition rate. The FCM results demonstrated that the Fuzheng Lishi Tang blocked the cell cycle in the G_2/M phase.Western blot results showed that the Fuzheng Lishi Tang could down-regulate protein expressions of bcl-2 and up-regulate the expression bax of PC-3 cells.Conclusion: The Serum Containing Fuzheng Lishi Tang can inhibit the proliferation of PC-3 cells in vitro by regulating the expression of Bcl-2 and Bax proteins.
Objective: To explore the effects of Fuzheng Lishi Tang on the proliferatio of prostate cancer PC-3 cell lines and exploring the mechanism. Methods: The inhibitory effects of Fuzheng Lishi Tang on growth of PC-3 cells were detected by using CCK-8 assay. The influence of Fuzheng Lishi Tang on apoptosis and cell cycle of PC-3 cells was detected by flow cytometer. The influence of Fuzheng Lishi Tang on protein expressions of bcl-2 and bax of PC-3 cells was analyzed by Western blotting method. Results: The Fuzheng Lishi Tang had inhibitory effect on proliferation of PC-3 cells, its effective time and dose were positively correlated to cellular proliferation inhibition rate. The FCM results demonstrated that the Fuzheng Lishi Tang blocked the cell cycle in the G_2/M phase.Western blot results showed that the Fuzheng Lishi Tang could down-regulate protein expressions of bcl-2 and up-regulate the expression bax of PC-3 cells.Conclusion: The Serum Containing Fuzheng Lishi Tang can inhibit the proliferation of PC-3 cells in vitro by regulating the expression of Bcl-2 and Bax proteins.
引文
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[2] 魏云飞,顾晓箭,朱清毅,等.去势抵抗性前列腺癌的治疗新进展[J].中华男科学杂,2016,22(5):455-461.
[3] Siegel RL,Miller KD,Jemal A.Cancer statistics,2016[J].CA Cancer J Clin,2016,66:7-30.
[4] 原小斌,王毅东,赵文兵,等.肾癌根治术后益气解毒方联合细胞因子辅助治疗临床疗效评估[J].山西医科大学学报,2016,47(1):74-79.
[5] 黄建锋,王鹏,王毅东,等.益气解毒方对ACHN 肾癌干细胞增殖及凋亡影响的实验研究[J].中国药物与临床,2016,16(2):187-188.
[6] 黄财顺,向诚,李宝才,等.基于中药血清药物化学的活性成分筛选的现状和问题[J].中草药,2014,45(20):3009.
[7] 王喜军.中药药效物质基础研究的系统方法学-中医方证代谢组学[J].中国中药杂志,2015,40(1):13.
[8] 卢磊,刘晓丹,张培影.中药血清药理学及血清药物化学研究进展[J].中国中医急症,2018,27(1):178-181,188.
[9] Mohsenikia M,Farhangi B,Alizadeh A M,et al.Therapeutic effects of dendrosomal solanine on a metastatic breast tumor [J].Life Sci,2016,148(7):260-267.
[10] Wang Q,Zhang L,Yuan X,et al.The Relationship between the Bcl-2 /Bax proteins and the mitochondria-mediated apoptosis pathway in the differentiation of adipose-derived stromal cells into neurons[J].PLoS One,2016,11(10):e0163327.