短光照对绒山羊绒毛生长相关激素的影响
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摘要
为研究控制光周期对绒山羊绒毛生长相关激素的影响,从短光照试验和对照组绒山羊中选择2周岁6对双胞胎绒山羊母羊,采集血液样品利用酶联免疫法进行激素含量测定,利用SAS9.0软件进行显著性检验和相关性分析。结果发现:一天24h当中,绒山羊进入棚圈后褪黑激素MLT(melatonin,mLT)和IGF-1含量显著增加,PRL含量显著减少,EGF和GH含量在2组中差异不显著;一年当中,进入光控棚圈后6月份绒山羊血液中的MLT(P<0.05)、IGF-1和EGF(P>0.05)含量均增加,PRL含量显著降低;9月份试验组绒山羊MLT、IGF-1和GH含量高于对照组(P<0.05),PRL含量依然低于对照组(P>0.05)。在相关性分析中,试验组和对照组PRL与其他激素之间的相关系数为负数;与对照组相比短光照明显增加了MLT与PRL、EGF,PRL与IGF-1、EGF、GH,IGF-1与EGF、GH,EGF与GH之间的相互作用(P>0.05),减弱了MLT与IGF-1、GH之间的相互作用。结果表明,短光照通过增强各激素间的协同或拮抗作用而提前诱发绒毛生长。
To investigate the effect of short photoperiod on hormones that related to hair growth in Inner Mongolia(Arbas type)Cashmere goat,six pairs of 2-year-old twins ewes were selected from the short photoperiod and control group.The blood samples were collected and content of hormones were determined by enzyme-linked immunoassay.SAS9.0 software was adopted to conduct significant test and correlation analysis.The results showed that the content of MLT and IGF-1 was significantly increased,the content of PRL was significantly decreased,and the content of EGF and GH was not significant different between the two groups under short photoperiod in a day.In a year,the content of MLT(P<0.05),IGF-1 and EGF(P>0.05)was increased,and the content of PRL(P<0.05)was significantly decreased under short photoperiod in June.The content of MLT,IGF-1 and GH was significantly higher(P<0.05),and the content of PRL was lower in the experimental group than in the control group,in September.The correlation between PRL and other hormones in both groups was negative.Compared with the control group,interactions between MLT and PRL/EGF,between PRL and IGF-1/EGF/GH,between IGF-1 and EGF/GH,between EGF and GH(P>0.05)were increased,and interactions between MLT and IGF-1/GH were decreased under short photoperiod.In conclusion,the short photoperiod might enhance hair growth by synergistic and antagonistic effect of hormones that related to hair growth.
To investigate the effect of short photoperiod on hormones that related to hair growth in Inner Mongolia(Arbas type)Cashmere goat,six pairs of 2-year-old twins ewes were selected from the short photoperiod and control group.The blood samples were collected and content of hormones were determined by enzyme-linked immunoassay.SAS9.0 software was adopted to conduct significant test and correlation analysis.The results showed that the content of MLT and IGF-1 was significantly increased,the content of PRL was significantly decreased,and the content of EGF and GH was not significant different between the two groups under short photoperiod in a day.In a year,the content of MLT(P<0.05),IGF-1 and EGF(P>0.05)was increased,and the content of PRL(P<0.05)was significantly decreased under short photoperiod in June.The content of MLT,IGF-1 and GH was significantly higher(P<0.05),and the content of PRL was lower in the experimental group than in the control group,in September.The correlation between PRL and other hormones in both groups was negative.Compared with the control group,interactions between MLT and PRL/EGF,between PRL and IGF-1/EGF/GH,between IGF-1 and EGF/GH,between EGF and GH(P>0.05)were increased,and interactions between MLT and IGF-1/GH were decreased under short photoperiod.In conclusion,the short photoperiod might enhance hair growth by synergistic and antagonistic effect of hormones that related to hair growth.
引文
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[20]ZHANG H,NAN W,WANG S,et al.Epidermal growth factor promotes proliferation and migration of follicular outer root sheath cells via Wnt/β-catenin signaling[J].Celularl physiology and biochemistry,2016,39(1):360-370.
[21]PASTORE S,MASCIA F,MARIANI V,et al.The epidermal growth factor receptor system in skin repair and inflammation[J].Journal of investigative dermatology,2008,128(6):1365-1374.
[22]王昊.辽宁绒山羊绒毛生长、血液中相关激素变化及皮肤中基因的表达分析[D].沈阳:沈阳农业大学,2016.
[23]ZHANG H,NAN W,WANG S,et al.Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway[J].Biochimie,2016,127:10-18.
[2]贾志海.不同光周期和褪黑激素对绒山羊生产性能影响[J].中国畜牧杂志,1995,31(4):8-10.
[3]FISCHER T W,SWEATMAN T W,SEMAK I,et al.Constitutive and UV-induced metabolism of melatonin in keratinocytes and cell-free systems[J].Faseb,2006,20(9):1564-1566.
[4]SLOMINSKI A,PISARCHIK A,ZBYTEK B,et al.Functional activity of serotoninergic and melatoninergic systems expressed in the skin[J].Journal of cellular physiology,2003,196(1):144-153.
[5]王林枫,杨改青,杨耀胜,等.光照和褪黑激素对非生绒期绒山羊激素分泌和绒毛生长的影响[J].中国畜牧兽医,2008,35(12):29-32.
[6]ROSE J,KENNEDY M,JOHNSTON B,et al.Serum prolactin and dehydroepiandrosterone concentrations during the summer and winter hair growth cycles of mink(Mustela vison)[J].Comparative biochemistry and physiology,Part A,molecular&integrative physiology,1998,121(3):263-271.
[7]SANTIAGO-MORENO J,LóPEZ-SEBASTIáN A,DEL CAMPOA,et al.Effect of constant-release melatonin implants and prolonged exposure to a long day photoperiod on prolactin secretion and hair growth in mouflon(Ovis gmelini musimon)[J].Domestic animal endocrinology,2004,26(4):303-314.
[8]SHAMSALDDINI S,MOHAMMADABADI M R,ESMAILIZA-DEH A K.Polymorphism of the prolactin gene and its effect on fiber traits in goat[J].Genetika,2016,52(4):405-408.
[9]HAASE I,EVANS R,POFAHL R,et al.Regulation of keratiaocyte shape,migration and wound epithelialization by IGF-land EGF-dependent signalling pathways[J].Journal of cell science,2003,116(15):3227-3238.
[10]王林枫,杨改青,张世军,等.光照和埋植褪黑激素对绒山羊相关激素分泌的影响[J].动物生产,2009,45(21):36-40.
[11]PASTORE S,MASCIA F,MARIANI V,et al.The epidermal growth factor receptor system in skin repair and inflammation[J].Journal of investigative dermatology,2008,128(6):1365-1374.
[12]孙福亮.新吉细毛羊和小尾寒羊的毛品质性状及皮肤转录组学研究[D].吉林:延边大学,2016.
[13]MANOUCHEHR S,LAYA G,MAHSA D.Effect of epidermal growth facter,platelet derived growth facter and growth hormone on cultured rat keratinocytes cells in vitro[J].Pakistan journal of biological sciences,2014,17(7):931-936.
[14]LIU B,GAO F Q,GUO J,et al.A Microarray-based analysis reveals that ashort photoperiod promotes hair growth in the Arbas Cashmere goat[J].PLoS ONE,2016,11(1):e0147124.
[15]KOBAYASHI H,KROMMINGA A,DUNLOP T W,et al.Arole of melatonin in neuroectodermal-mesodermal interactions:the hair follicle synthesizes melatonin and expresses functional melatonin receptors[J].Faseb journal,2005,19(12):1710-1712.
[16]MOELLMANN G,LERNER A B,HENDEE J R.The mechanism of frog skin lightening by acetylcholine[J].General&comparative endocrinology,1974,23(1):45-51.
[17]BERLINGUER F,LEONI G G,SUCCU S,et al.Exogenous melatonin positively influences follicular dynamics,oocyte developmental competence and blastocyst output in a goat model[J].Journal of pineal research,2009,46(4):383-391.
[18]TENORIO F D,SIM7ES M J,TEIXEIRA V W,et al.Effects of melatonin and prolactin in reproduction:review of literature[J].Revista da associacao medica brasileira,2015,61(3):269-274.
[19]CASTELA M,LINAY F,ROY E,et al.IGF1Rsignaling acts on the anagen to catagen transition in the hair cycle[J].Exprimental dermatology,2017,17.doi:10.1111/exd.13287.
[20]ZHANG H,NAN W,WANG S,et al.Epidermal growth factor promotes proliferation and migration of follicular outer root sheath cells via Wnt/β-catenin signaling[J].Celularl physiology and biochemistry,2016,39(1):360-370.
[21]PASTORE S,MASCIA F,MARIANI V,et al.The epidermal growth factor receptor system in skin repair and inflammation[J].Journal of investigative dermatology,2008,128(6):1365-1374.
[22]王昊.辽宁绒山羊绒毛生长、血液中相关激素变化及皮肤中基因的表达分析[D].沈阳:沈阳农业大学,2016.
[23]ZHANG H,NAN W,WANG S,et al.Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway[J].Biochimie,2016,127:10-18.