鱼腥草芩蓝口服液质量控制方法研究
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摘要
为建立鱼腥草芩蓝口服液的质量控制方法,采用TLC法对鱼腥草芩蓝口服液中的鱼腥草、黄芩、连翘和金银花进行鉴别;采用HPLC法测定黄芩苷和绿原酸的含量。在薄层色谱鉴别中均能检出鱼腥草、黄芩、连翘和金银花的特征性斑点;黄芩苷进样量在0.1057～2.6413μg范围内与峰面积有良好的线性关系,回归方程为:Y=33.42325X-214.6128,r=0.9997,平均回收率为98.74%;绿原酸进样量在0.041～2.051μg/m L范围内与峰面积有良好的线性关系,其回归方程为:Y=-86.78088+27.5937X,相关系数r=0.9995,回归显著,平均回收率为99.1%,建立的黄芩苷和绿原酸含量测定方法,耐受性良好、灵敏度高、重现性好、专属性强,可以有效控制产品的质量。建立的方法能准确、可靠地对鱼腥草芩蓝口服液中黄芩苷和绿原酸含量进行测定,可作为该制剂的质量控制方法。
To establish a quality control method for Yuxingcao Qinlan oral liquid. Houttuynia cordata,Astragalus membranaceus,Forsythia suspensa and Honeysuckle in Yuxingcao Qinlan oral Liquid were identified by TLC method. The content of baicalin and chlorogenic acid was determined by HPLC. The characteristic spots of Houttuynia cordata,Astragalus membranaceus,Forsythia suspensa and Honeysuckle can be detected in the thin layer chromatography. The amount of baicalin injection in the range of 0. 1057 ～ 2. 6413 μg has a good linear relationship with the peak area. The regression equation is: Y = 33.42325 X-214.6128,r = 0.9997,the average recovery was 98.74%; the chlorogenic acid injection amount in the range of 0. 041 ～ 2. 051 μg/m L had a good linear relationship with the peak area,and the regression equation was: Y =-86. 78088 + 27. 5937 X,correlation coefficient r = 0.9995,significant regression,average recovery rate is 99.1%,the determination method of baicalin content and chlorogenic acid content established in the standard,good tolerance,high sensitivity,reproducibility Good,specific,can effectively control the quality of the product. The method could accurately and reliably determine the content of baicalin and chlorogenic acid in Yuxingcao Qinlan oral Liquid,and could be used as a quality control method for the preparation.
To establish a quality control method for Yuxingcao Qinlan oral liquid. Houttuynia cordata,Astragalus membranaceus,Forsythia suspensa and Honeysuckle in Yuxingcao Qinlan oral Liquid were identified by TLC method. The content of baicalin and chlorogenic acid was determined by HPLC. The characteristic spots of Houttuynia cordata,Astragalus membranaceus,Forsythia suspensa and Honeysuckle can be detected in the thin layer chromatography. The amount of baicalin injection in the range of 0. 1057 ～ 2. 6413 μg has a good linear relationship with the peak area. The regression equation is: Y = 33.42325 X-214.6128,r = 0.9997,the average recovery was 98.74%; the chlorogenic acid injection amount in the range of 0. 041 ～ 2. 051 μg/m L had a good linear relationship with the peak area,and the regression equation was: Y =-86. 78088 + 27. 5937 X,correlation coefficient r = 0.9995,significant regression,average recovery rate is 99.1%,the determination method of baicalin content and chlorogenic acid content established in the standard,good tolerance,high sensitivity,reproducibility Good,specific,can effectively control the quality of the product. The method could accurately and reliably determine the content of baicalin and chlorogenic acid in Yuxingcao Qinlan oral Liquid,and could be used as a quality control method for the preparation.
引文
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[16]童文,李敏,孙佩,等.药食同源植物鱼腥草研究现状[J].辽宁中医药大学学报,2018,20(6):183-185.Tong W,Li M,Sun P,et al.Research status of houttuynia cordata houttuyniae[J].Journal of Liaoning University of Traditional Chinese Medicine,2018,20(6):183-185.
[17]田丁,史梦琪,王赟.连翘挥发油化学成分及其药理作用研究进展[J].天然产物研究与开发.2018,30:1834-1842.Tian D,Shi M Q,Wang B.Advances in chemical constituents and pharmacological activities of essential oils from forsythia suspensa L[J].Natural Product Research and Developmen,2018,30:1834-1842.
[18]陆安,刘炳炜,杜红娜,等.鱼腥草芩蓝口服液指纹图谱研究[J].中国兽药杂志,2018,52(12):61-68.Lu A,Liu B W,Du H N,et al.Studies on HPLC fingerprint of Yuxingcaoqinlan Oral Liquid[J].Chinese Journal of Veterinary Drug,2018,52(12):61-68.
[2]喻琴,王东升,张世栋,等.鱼腥草芩蓝口服液抗炎镇痛作用研究[J].动物医学进展,2018,39(8):36-39.Yu Q,Wang D S,Zhang S D,et al.Study on anti-inflammatory and analgesic activities of Yuxingcao Qinlan oral liquid[J].Progress in Veterinary Medicine,2018,39(8):36-39.
[3]中华人民共和国农业部公告第2653号.The ministry of agriculture of the People's Republic of China,No.2653.
[4]喻琴,陈玲,尹伶灵,等.鱼腥草芩蓝口服液的毒理学研究[J].中国兽药杂志,2018,52(9):27-36.Yu Q,Chen L,Yin L L,et al.Toxicological study of houttuynia cordata and Qinlan oral liquid[J].China Journal of Veterinary Medicine,2018,52(9):27-36.
[5]中国兽药典委员会.《中华人民共和国兽药典》2015年版二部[S].China Veterinary Pharmacopoeia Committee.Peopie's Republic of China Veterinary Pharmacopoeia volumeⅡ2015 edition[S].
[6]周宝玉,虞文殊.复方鱼腥草胶囊中连翘苷和黄芩苷的高效液相色谱法含量检测[J].世界中医药,2018,13(12):3186-3189.Zhou B Y,Yu W S.Determination of forsythiaside and baicalin in compound houttuynia capsules by high performance liquid chromatography[J].World Chinese Medicine,2018,13(12):3186-3189.
[7]朱育红,潘康锁,林涛,等.高效液相色谱法测定蒲地蓝消炎颗粒中黄芩苷的含量[J].中国兽药杂志,2018,52(12):49-53.Zhu Y H,Pan K S,et al.The content of baicalin in putilan antiinflammatory granule determined by HPLC[J]Chinese Journal of Veterinary Drug,2018,52(12):49-53.
[8]陈红,邓萌萌,周波,等.复方J含片中金银花的薄层鉴别及绿原酸的高效液相色谱含量测定[J].四川中医,2018,36(11):192-195.Chen H,Deng M M,Zhou B,et al.TLC identification of flos lonicerae in compound buccal tablets and determination of chlorogenic acid by high performance liquid chromatography[J].Journal of Sichuan of Traditional Chinese Medicine,2018,36(11):192-195.
[9]高海燕.HPLC双波长法同时测定小儿咳喘灵颗粒中绿原酸与苦杏仁苷的含量[J].亚太传统医药,2018,14(12):42-44.Gao H Y.Simultaneous determination of chlorogenic acid and amygdalin in Xiaoer Kechuanling granules by dual-wavelength HPLC[J].Journal of Asia-Pacific Traditional Medic,2018,14(12):42-44.
[10]周争道,张莉,周慧云,等.HPLC同时测定消炎利胆片中5种活性成分的含量[J].中国现代应用药学,2018,35(12):1801-1804.Zhou Z D,Zhang L,Zhou H Y,et al.Simultaneous determination of five active ingredients in Xiaoyan Lidan Tablets by HPLC[J].Chinese Journal of Modern Applied Pharmac,2018,35(12):1801-1804.
[11]李春雪,张晨,王长生,等.菊黄口服液质量控制方法研究[J].中草药,2015,46(12):1779-1785.Li C X,Zhang C,Wang C S,et al.Research on quality control method for Juhuang Oral Liquid[J].Chinese Traditional and Herbal Drugs,2015,46(12):1779-1785.
[12]石新华,杨柳,陈红.芩栀鱼合剂的薄层色谱鉴别[J].时珍国医国药,2014,25(2):374-375.Shi Y H,Yang L,Chen H.Lishizhen medicine and materia medica research,2014,25(2):374-375.
[13]杨秀青,石征蓉,谷江华,等.银菊解毒口服液指纹图谱结合多指标成分定量测定的质量控制方法研究[J].中草药,2017,48(13):2645-2652.Yang X Q,Shi Z R,Gu J H,et al.Quality assessment of Yinju Jiedu Oral Liquid based on HPLC fingerprint and multi-components simultaneous determination[J].Chinese Traditional and Herbal Drugs,2017,48(13):2645-2652.
[14]陈晓亮,汪旭.栀芩合剂质量控制方法的研究[J].安徽医药,2017,21(1):38-41.Chen X L,Wang X.Quality control method of zhiqin mixture[J].Anhui Medical and Pharmaceutical Journal,2017,21(1):38-41.
[15]赵惠茹,路荣荣,靖会,等.金银花中绿原酸超声提取工艺的优化[J].化工科技,2018,26(6):22-26.Zhao H r,Lu R R,Jing H,et al.Optimization of extraction technology of chlorogenic acid from Lonicera japonica by ultrasonic method[J].Science and Technology chemical industry,2018,26(6):22-26.
[16]童文,李敏,孙佩,等.药食同源植物鱼腥草研究现状[J].辽宁中医药大学学报,2018,20(6):183-185.Tong W,Li M,Sun P,et al.Research status of houttuynia cordata houttuyniae[J].Journal of Liaoning University of Traditional Chinese Medicine,2018,20(6):183-185.
[17]田丁,史梦琪,王赟.连翘挥发油化学成分及其药理作用研究进展[J].天然产物研究与开发.2018,30:1834-1842.Tian D,Shi M Q,Wang B.Advances in chemical constituents and pharmacological activities of essential oils from forsythia suspensa L[J].Natural Product Research and Developmen,2018,30:1834-1842.
[18]陆安,刘炳炜,杜红娜,等.鱼腥草芩蓝口服液指纹图谱研究[J].中国兽药杂志,2018,52(12):61-68.Lu A,Liu B W,Du H N,et al.Studies on HPLC fingerprint of Yuxingcaoqinlan Oral Liquid[J].Chinese Journal of Veterinary Drug,2018,52(12):61-68.