500份HIV 1/2抗体蛋白印迹试验阳性样本的带型分析
|
摘要
目的分析2010—2017年丹东地区艾滋病病毒(HIV)1/2抗体蛋白印迹试验(WB)阳性样本的带型分布规律,为制定该地区切实有效的艾滋病防控措施提供科学依据。方法采用WB对2010—2017年500份HIV-1抗体筛查阳性样本进行确证检测,并对WB带型进行统计分析。结果 500份HIV-1抗体阳性样本中,WB带型组合为全带型和次全带的占90.0%(450份);抗env、抗pol基因编码的抗体条带和抗gag基因编码的p24检出率均超过93.0%,p55和p17检出率分别为68.8%和85.2%。感染者以男性居多,p24在不同性别中检出率的差异有统计学意义(χ~2=6.706,P<0.05);抗env基因编码的抗体在各个年龄组的检出率差异均无统计学意义(P>0.05),抗pol基因编码的抗体在各个年龄组的检出率差异均有统计学意义(P<0.05),抗gag基因编码的抗体除p55外,p24和p17在各个年龄组的检出率差异均有统计学意义(P<0.05)。结论该地区大部分感染者在艾滋病期前被及时发现,中青年人群为该地区HIV感染的高危人群,应重点防控。
Objective To analyze the pattern distribution of HIV-1/2 antibody positive samples tested by western blot(WB)in Dandong city,to provide effective measures for HIV/AIDS prevention and control.Methods We used WB to detect 500 HIV-1 antibody positive samples from 2010 to 2017,and statistical analysis was made to know the WB belt types.Results Out of 500 HIV-1 antibody positive samples,WB band was mainly composed of full band and sub band,accounting for 90.0%(450).The detection rate of the antibodies encoded by anti-envgene and anti-pol genes was more than 93%,as well as p24 encoded by anti-gag gene.The detection rate of p55 and p17 was 68.8% and 85.2% respectively.Patients were mainly male,and the difference of p24 detection rate in different genders was statistically significant(χ~2=6.706,P<0.05).The detection rate of anti-Env genes encoded antibodies was not statistically significant in all age groups(P>0.05),but the antibodies encoded by anti-pol genes were statistically different(P<0.05).The differences of detection rate of antibodies encoded by anti-gag genes of p24 and p17,in spite of p55,were statistically significant in all age groups(P<0.05).Conclusion Most of the people in Dandong are early detected,showing the current HIV/AIDS prevention and control measures are effective.Young and middle-aged people are at high risk of HIV infection,and are our priority groups for HIV/AIDS prevention and control.
Objective To analyze the pattern distribution of HIV-1/2 antibody positive samples tested by western blot(WB)in Dandong city,to provide effective measures for HIV/AIDS prevention and control.Methods We used WB to detect 500 HIV-1 antibody positive samples from 2010 to 2017,and statistical analysis was made to know the WB belt types.Results Out of 500 HIV-1 antibody positive samples,WB band was mainly composed of full band and sub band,accounting for 90.0%(450).The detection rate of the antibodies encoded by anti-envgene and anti-pol genes was more than 93%,as well as p24 encoded by anti-gag gene.The detection rate of p55 and p17 was 68.8% and 85.2% respectively.Patients were mainly male,and the difference of p24 detection rate in different genders was statistically significant(χ~2=6.706,P<0.05).The detection rate of anti-Env genes encoded antibodies was not statistically significant in all age groups(P>0.05),but the antibodies encoded by anti-pol genes were statistically different(P<0.05).The differences of detection rate of antibodies encoded by anti-gag genes of p24 and p17,in spite of p55,were statistically significant in all age groups(P<0.05).Conclusion Most of the people in Dandong are early detected,showing the current HIV/AIDS prevention and control measures are effective.Young and middle-aged people are at high risk of HIV infection,and are our priority groups for HIV/AIDS prevention and control.
引文
[1]中国疾病预防控制中心.全国艾滋病检测技术规范(2009年、2015年修订版)[S].2009,2015.
[2]陈云钰,潘超,邓陈哲,等.HIV筛查阳性标本免疫印迹试验结果分析[J].现代预防医学,2012,39(21):5667-5668,5672.
[3]Tugizov S.Human immunodeficiency virus-associated disruption of mucosal barriers and its role in HIV transmission and pathogenesis of HIV/AIDS disease[J].Tissue Barriers,2016,4(3):e1159276.
[4]郭志宏,徐宝祥,严菊英,等.HIV初筛阳性标本确认试验结果分析[J].中国性病艾滋病防治,1997,3(3):116-117.
[5]Wang JB,Zhang N,Yu HY,et al.Study on the role of western blot band profile for the detection of recent HIV infection[J].Zhonghua Liu Xing Bing Xue Za Zhi,2013,34(10):998-1002.
[6]刘燕,严宇斌,曾健君,等.113例男性HIV-1抗体阳性吸毒者蛋白免疫印迹分析[J].中国热带医学,2011,11(11):1330-1331.
[7]王同展,尤向东,吕翠霞,等.山东省2003年至2005年艾滋病确认实验室检测指标分析[J].中国卫生检测杂志,2007,2(17):314-315.
[8]苏雪銮,陈爱苗,谢璧珠,等.广东潮州地区HIV-1抗体阳性者免疫印迹带型分析[J].中国热带医学,2014,14(10):1254-1256.
[9]李燕,梁彩云,高凯,等.265例HIV-1抗体阳性者抗体蛋白印迹带型分析[J].中国公共卫生,2005,21(11):1293-1294.
[10]Soong J,Riley R,Mcpherson R.Oligoclonal Bands of Immunoglobulins in Serum Leading to Diagnosis of Human Immunodeficiency Virus 1Infection[J].Am J Clin Pathol,2016,145(2):277-281.
[11]李楠,缪礼锋.WB带型分析对HIV感染期和AIDS期的诊断意义[J].疾病控制杂志,2005,9(4):376-377.
[12]张佳峰,郭志宏,姚亚萍,等.1 155例的HIV-1抗体阳性者免疫印迹带型分析及规律总结[J].中国卫生检验杂志,2010,20(9):2247-2249.
[13]黎俊宏,徐晓怡,鲍东平,等.2011—2014年常州地区HIV-1抗体阳性者免疫免疫印迹带型分析[J].现代预防医学,2015,42(22):4156-4158.
[14]叶灵,雷永良,梅建华,等.丽水市HIV-1抗体阳性人群WB带型分析[J].中国艾滋病性病,2017,23(3):190-193.
[15]钟敏,马莎,杨绍敏.367例HIV-1阳性样本WB确认结果的带型分析[J].检验医学与临床,2012,9(20):2529-2530.
[16]刘昕亮,牟怀德,徐蓉芳.2007—2012年乐山地区HIV-1抗体阳性标本蛋白免疫印迹带型分析[J].现代预防医学,2015,42(18):3380-3383.
[17]郭川,纪琳莹,吴耀波,等.不同临床分期HIV-1感染者/AIDS患者血样的蛋白印迹试验带型分析[J].检验医学,2015,30(2)145-148.
[18]史晓林,王峰,刘双辉,等.140例HIV-1抗体阳性者WB带型及CD4+T细胞结果分析[J].中国卫生检验杂志,2009,19(11):2600-2602.
[2]陈云钰,潘超,邓陈哲,等.HIV筛查阳性标本免疫印迹试验结果分析[J].现代预防医学,2012,39(21):5667-5668,5672.
[3]Tugizov S.Human immunodeficiency virus-associated disruption of mucosal barriers and its role in HIV transmission and pathogenesis of HIV/AIDS disease[J].Tissue Barriers,2016,4(3):e1159276.
[4]郭志宏,徐宝祥,严菊英,等.HIV初筛阳性标本确认试验结果分析[J].中国性病艾滋病防治,1997,3(3):116-117.
[5]Wang JB,Zhang N,Yu HY,et al.Study on the role of western blot band profile for the detection of recent HIV infection[J].Zhonghua Liu Xing Bing Xue Za Zhi,2013,34(10):998-1002.
[6]刘燕,严宇斌,曾健君,等.113例男性HIV-1抗体阳性吸毒者蛋白免疫印迹分析[J].中国热带医学,2011,11(11):1330-1331.
[7]王同展,尤向东,吕翠霞,等.山东省2003年至2005年艾滋病确认实验室检测指标分析[J].中国卫生检测杂志,2007,2(17):314-315.
[8]苏雪銮,陈爱苗,谢璧珠,等.广东潮州地区HIV-1抗体阳性者免疫印迹带型分析[J].中国热带医学,2014,14(10):1254-1256.
[9]李燕,梁彩云,高凯,等.265例HIV-1抗体阳性者抗体蛋白印迹带型分析[J].中国公共卫生,2005,21(11):1293-1294.
[10]Soong J,Riley R,Mcpherson R.Oligoclonal Bands of Immunoglobulins in Serum Leading to Diagnosis of Human Immunodeficiency Virus 1Infection[J].Am J Clin Pathol,2016,145(2):277-281.
[11]李楠,缪礼锋.WB带型分析对HIV感染期和AIDS期的诊断意义[J].疾病控制杂志,2005,9(4):376-377.
[12]张佳峰,郭志宏,姚亚萍,等.1 155例的HIV-1抗体阳性者免疫印迹带型分析及规律总结[J].中国卫生检验杂志,2010,20(9):2247-2249.
[13]黎俊宏,徐晓怡,鲍东平,等.2011—2014年常州地区HIV-1抗体阳性者免疫免疫印迹带型分析[J].现代预防医学,2015,42(22):4156-4158.
[14]叶灵,雷永良,梅建华,等.丽水市HIV-1抗体阳性人群WB带型分析[J].中国艾滋病性病,2017,23(3):190-193.
[15]钟敏,马莎,杨绍敏.367例HIV-1阳性样本WB确认结果的带型分析[J].检验医学与临床,2012,9(20):2529-2530.
[16]刘昕亮,牟怀德,徐蓉芳.2007—2012年乐山地区HIV-1抗体阳性标本蛋白免疫印迹带型分析[J].现代预防医学,2015,42(18):3380-3383.
[17]郭川,纪琳莹,吴耀波,等.不同临床分期HIV-1感染者/AIDS患者血样的蛋白印迹试验带型分析[J].检验医学,2015,30(2)145-148.
[18]史晓林,王峰,刘双辉,等.140例HIV-1抗体阳性者WB带型及CD4+T细胞结果分析[J].中国卫生检验杂志,2009,19(11):2600-2602.