高海拔生境下怀玉山高山马铃薯和怀玉山本土农家薯块茎的转录组分析
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摘要
为探究高海拔生境下怀玉山高山马铃薯的适应机制,本研究以怀玉山高山马铃薯(麻籽洋芋)和怀玉山本土农家薯的采后块茎为试验材料进行转录组分析。结果表明,怀玉山高山马铃薯上调的差异基因956个,下调的差异基因2 262个,总数为3 218个;怀玉山高山马铃薯和怀玉山本土农家薯与叶绿素结合、光系统Ⅰ、光合作用和光捕获、光系统Ⅱ、血红素结合、氧化还原酶活性等光合系统相关的GO term富集显著;怀玉山高山马铃薯和怀玉山本土农家薯与光合作用-天线蛋白、丙烷合成、光合作用、苯丙氨酸代谢等Pathway也富集显著,说明怀玉山高山马铃薯和怀玉山本土农家薯在怀玉山高海拔生境下主要是涉及到光合作用基因的差异表达。本研究结果为怀玉山高山马铃薯适应高海拔生境相关基因的挖掘与应用提供了一定的理论参考。
To explore the adaptation mechanism of alpine potato in Huaiyushan under high altitude habitats, transcriptional analysis was carried out with the tubers of alpine potato and local farm potato in Huaiyushan in this study. The results showed that there were 3 218 differentially expressed genes, among which 956 genes were up-regulated, 2 262 genes were down-regulated genes. GO terms associated with chlorophyll binding, photosystem Ⅰ, photosynthesis and light harvesting, photosystem Ⅱ, heme binding and oxidoreductase activity in alpine potato and local farm potato in Huaiyushan were significantly enriched. Pathways associated with photosynthesis-antenna protein, propane synthesis, photosynthesis, phenylalanine metabolism alpine potato and local farm potato in Huaiyushan were also significantly enriched, which indicated that in high altitude habitats, the differential expressed genes between alpine potato and local farm potato in Huaiyushan are mainly those related to photosynthesis genes. The results can provide reference for mining and application of the genes related to adaption to high altitude habitats in alpine potato in Huaiyushan.
To explore the adaptation mechanism of alpine potato in Huaiyushan under high altitude habitats, transcriptional analysis was carried out with the tubers of alpine potato and local farm potato in Huaiyushan in this study. The results showed that there were 3 218 differentially expressed genes, among which 956 genes were up-regulated, 2 262 genes were down-regulated genes. GO terms associated with chlorophyll binding, photosystem Ⅰ, photosynthesis and light harvesting, photosystem Ⅱ, heme binding and oxidoreductase activity in alpine potato and local farm potato in Huaiyushan were significantly enriched. Pathways associated with photosynthesis-antenna protein, propane synthesis, photosynthesis, phenylalanine metabolism alpine potato and local farm potato in Huaiyushan were also significantly enriched, which indicated that in high altitude habitats, the differential expressed genes between alpine potato and local farm potato in Huaiyushan are mainly those related to photosynthesis genes. The results can provide reference for mining and application of the genes related to adaption to high altitude habitats in alpine potato in Huaiyushan.
引文
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[17] 陈菁,郑伟,王谈笑,王炜,徐晓丹.钩苞大丁草高通量转录组测序及差异表达分析[J].西北植物学报,2017,37(3):470-477
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[19] 陈栋,宋海岩,龚荣高,李靖,涂美艳,江国良,孙淑霞,汤浩茹.红肉枇杷和白肉枇杷果实成熟不同阶段的比较转录组研究[J].分子植物育种,2017,15(6):2112-2118
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[24] 宁宇,董树斌,武高洁,王义飞,马骅,索朗夺尔基.基于RNA-seq西藏嵩草转录组分析[J].林业科技通讯,2017(7):27-32
[25] 李锦,严潘瑶,钱飞箭,邱宝义,夏雯雯,牟晓威,邱丽娟,林忠平,陈铭,祝建波,陈贤丰.新疆天山雪莲转录组注解知识库[J].植物学报,2017,52(4):530-538
[26] 张得芳,夏涛,文怀秀,王占林,刘霞,张磊,白灵娜.不同海拔下花叶海棠叶片转录组核苷酸变异分析和比较[J].基因组学与应用生物学,2017,36(10):4351-4356
[27] Ansorge W J.Next-generation DNA sequencing techniques[J].New Biotechnology,2009,25(4):195-203
[28] Xu Q,Yu K Q,Zhu A D,Ye J L,Liu Q,Zhang J C,Deng X X.Comparative transcripts profiling reveals new insight into molecular processes regulating lycopene accumulation in a sweet orange (Citrus sinensis ) red-flesh mutant[J].BMC Genomics,2009,10(1):540-555
[2] 宋西娇,谢礼,宣裕吉,王芳,严成其,陈剑平.复合侵染的马铃薯花叶病病原诊断[J].浙江农业学报,2018,30(1):99-105
[3] 姚志,谢云.主粮化背景下高山马铃薯产业化困境研究—以恩施市为例 [J].长江大学学报(自然科学版),2016,13(3):54-56
[4] 尹明华,刘燕,郁雪婷,李远芳,周榕,范亚红,罗玉婕,万小霞.怀玉山高山马铃薯茎尖再生苗6种病毒的DAS-ELISA检测与分析 [J].浙江农业学报,2017,29(10):1699-1705
[5] 汪颀浩,文欢,张大燕,王伟,彭成,高继海.两种天麻种子的转录组学分析[J].中药材,2017,40(12):2761-2766
[6] Progar Ⅴ,Jak?e J,?tahner N,Radi?ek S,Javornik B,Berne S.Comparative transcriptional analysis of hop responses to infection with Verticillium nonalfalfae[J].Plant Cell Reports,2017,36(10):1599-1613
[7] Metzker M L.Sequencing technologies-the next generation[J].Nature Reviews Genetics,2010,11(1):31-46
[8] 窦孝锐.马铃薯转录组测序研究进展[J].现代农业科技,2015,(13):81,83
[9] 牛显飞,蒋学飞,解艳芳,刘双双,王茂林.甘蓝型油菜矮化突变体的茎秆发育转录组分析[J].四川大学学报(自然科学版),2018,55(1):201-206
[10] 张少平,张少华,邱珊莲,张帅,吴松海,何炎森,郑开斌,郑加协.基于转录组测序的紫背天葵花青素相关基因分析[J].核农学报,2018,32(4):639-645
[11] 黄娟,邓娇,陈庆富.荞麦根的转录组学分析及黄酮合成基因的鉴定[J].中国农业科技导报,2017,19(2):9-19
[12] Zhang J Z,Ai X Y,Sun L M,Zhang D L,Guo W W,Deng X X,Hu C G.Transcriptome profile analysis of flowering molecular processes of early flowering trifoliate orange mutant and the wild-type [Poncirus trifoliata (L.) Raf.] by massively parallel signature sequencing[J].BMC Genomics,2011,12(1):63-83
[13] Ai X Y,Lin G,Sun L M,Hu C G,Guo W W,Deng X X,Zhang J Z.A global view of gene activity at the flowering transition phase in precocious trifoliate orange and its wild-type [Poncirus trifoliata (L.) Raf.] by transcriptome and proteome analysis[J].Gene,2012,510(1):47-58
[14] Zhang Y,Zhu J,Dai H.Characterization of transcriptional differences between columnar and standard apple trees using RNA-Seq[J].Plant Molecular Biology Reporter,2012,30(4):957-965
[15] Ikegami H,Habu T,Mori K,Nogata H,Hirata C,Hirashima K,Tashiro K,Kuhara S.De novo sequencing and comparative analysis of expressed sequence tags from gynodioecious fig (Ficus carica L.) fruits:Caprifig and Common fig[J].Tree Genetics & Genomes,2013,9(4):1075-1088
[16] 宋菊,黄剑,李志栋,龙月红,邢朝斌.多穗柯转录组分析及黄酮类化合物合成相关基因的挖掘[J].中国中药杂志,2017,42(4):675-679
[17] 陈菁,郑伟,王谈笑,王炜,徐晓丹.钩苞大丁草高通量转录组测序及差异表达分析[J].西北植物学报,2017,37(3):470-477
[18] Xie F,Burklew C E,Yang Y,Liu M,Xiao P,Zhang B,Qiu D.De novo sequencing and a comprehensive analysis of purple sweet potato (Impomoea batatas L.) transcriptome[J].Planta,2012,236(1):101-113
[19] 陈栋,宋海岩,龚荣高,李靖,涂美艳,江国良,孙淑霞,汤浩茹.红肉枇杷和白肉枇杷果实成熟不同阶段的比较转录组研究[J].分子植物育种,2017,15(6):2112-2118
[20] 林艺华,吴小斌,郑涛,林宗铿,陈振东.不同花色印度野牡丹转录组功能注释和分析[J].分子植物育种,2017,15(6):2133-2138
[21] 刘莉,舒江平,韦宏金,张锐,沈慧,严岳鸿.东亚特有珍稀蕨类植物岩穴蕨(碗蕨科)高通量转录组测序及分析[J].生物多样性,2016,24(12):1325-1334
[22] 黄玉兰,殷奎德,向君亮.薏苡幼苗叶片转录组分析[J].农业生物技术学报,2017,25(3):386-396
[23] Cao Q H,Li A,Chen J Y,Sun Y,Tang J,Zhang A,Zhou Z L,Zhao D L,Ma D F,Gao S.Transcriptome sequencing of the sweet potato progenitor [Ipomoea trifida (HBK) G.Don.] and discovery of drought tolerance genes[J].Tropical Plant Biology,2016,9 (2):63-72
[24] 宁宇,董树斌,武高洁,王义飞,马骅,索朗夺尔基.基于RNA-seq西藏嵩草转录组分析[J].林业科技通讯,2017(7):27-32
[25] 李锦,严潘瑶,钱飞箭,邱宝义,夏雯雯,牟晓威,邱丽娟,林忠平,陈铭,祝建波,陈贤丰.新疆天山雪莲转录组注解知识库[J].植物学报,2017,52(4):530-538
[26] 张得芳,夏涛,文怀秀,王占林,刘霞,张磊,白灵娜.不同海拔下花叶海棠叶片转录组核苷酸变异分析和比较[J].基因组学与应用生物学,2017,36(10):4351-4356
[27] Ansorge W J.Next-generation DNA sequencing techniques[J].New Biotechnology,2009,25(4):195-203
[28] Xu Q,Yu K Q,Zhu A D,Ye J L,Liu Q,Zhang J C,Deng X X.Comparative transcripts profiling reveals new insight into molecular processes regulating lycopene accumulation in a sweet orange (Citrus sinensis ) red-flesh mutant[J].BMC Genomics,2009,10(1):540-555