摘要
通过高温热解的方法制备出Mn3O4纳米粒子,再利用正硅酸四乙酯(TEOS)包硅改善其水溶性和稳定性,通过加入3-氨基丙基三乙氧基硅烷(APTES)使纳米粒子表面接入大量氨基,最后再连接增加磁性纳米粒子生物相容性的有机分子聚乙二醇(PEG)和靶向分子叶酸(FA),得到Mn3O4靶向造影剂.体外实验表明,该造影剂具有低的细胞毒性,并对宫颈癌细胞具有较好的磁共振增强成像效果以及主动靶向作用.
The monodisperse silica-coated manganese oxide nanoparticles(NPs) were synthesized via a thermal decomposition approach and were aminated through silanization. The amine-functionalized core-shell NPs enabled the covalent conjugation of organic hydrophilic PEG and folate(FA) onto their surfaces. The formed Mn3O4@SiO2-PEG-FA core-shell nanocomposites are water-dispersible, stable, and biocompatible. Furthermore, we demonstrate that Mn3O4@SiO2-PEG-FA NPs as a T1 contrast agent display effective targeting ability, and lower toxicity in vitro, enabling them to be developed as multimodel nanoplatform for long-term targeted imaging.
引文
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