正交试验法优选山黄祛痛喷雾剂醇提工艺
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摘要
目的优选山黄祛痛喷雾剂醇提工艺。方法采用L9(34)正交试验法考察乙醇浓度、乙醇用量、回流时间、回流次数4个因素在不同水平对秋水仙碱、槲皮素含量和出膏率的影响。结果影响因素按程度大小依次为回流次数、乙醇浓度、回流时间和乙醇用量;山黄祛痛喷雾剂最佳醇提工艺为丽江山慈菇和凤仙透骨草采用8倍量70%乙醇,回流提取2次,每次1. 5 h。结论该醇提工艺稳定可行、质量可控,可用于山黄祛痛喷雾剂的制备。
Objective To optimize the ethanol extraction process of Shanhuang Qutong Spray. Methods L9( 34) orthogonal test was used to investigate the effects of different levels of ethanol concentration,ethanol consumption,reflux time and reflux times on the content and the extraction rate of colchicine and quercetin. Results The influencing factors were reflux times,ethanol concentration,reflux time and ethanol consumption in order of the influence level. The optimum ethanol extraction process of Shanhuang Qutong Spray was as follows:Iphigenia indica and Impatiens balsamina were extracted with 8 times of 70% ethanol,reflux extraction for 2 times,and 1. 5 h each time. Conclusion The ethanol extraction process is stable and feasible,the quality is controllable. It can be used for the preparation of Shanhuang Qutong Spray.
Objective To optimize the ethanol extraction process of Shanhuang Qutong Spray. Methods L9( 34) orthogonal test was used to investigate the effects of different levels of ethanol concentration,ethanol consumption,reflux time and reflux times on the content and the extraction rate of colchicine and quercetin. Results The influencing factors were reflux times,ethanol concentration,reflux time and ethanol consumption in order of the influence level. The optimum ethanol extraction process of Shanhuang Qutong Spray was as follows:Iphigenia indica and Impatiens balsamina were extracted with 8 times of 70% ethanol,reflux extraction for 2 times,and 1. 5 h each time. Conclusion The ethanol extraction process is stable and feasible,the quality is controllable. It can be used for the preparation of Shanhuang Qutong Spray.
引文
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[2]陈秀梅,钱士辉,冯锋.凤仙透骨草的化学成分[J].药学与临床研究,2009,17(1):31-33.
[3]张永峰,曾常茜,黄雯,等.透骨草化学成分和药理学作用研究进展[J].中华中医药学刊,2010,28(9):1960-1962.
[4]云南省食品药品监督管理局.云南省中药材标准[M].昆明:云南科技出版社,2005:57.
[5]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,1977:119.
[6]虞阳,林新艳,唐雯慧,等.高效液相色谱法测定山黄祛痛喷雾剂中秋水仙碱含量[J].中国药业,2016,25(2):59-61.
[7]袁理春,徐中志,赵琪,等.丽江山慈姑秋水仙碱HPLC测定[J].西南农业学报,2007,20(1):120-122.
[8]王晖,刘良玉,王林江. HPLC法测定透骨草中槲皮素的含量[J].江西中医学院学报,2005,17(4):41.
[9]蒙龙,李娟,龙锐.小剂量与常规剂量秋水仙碱治疗急性痛风性关节炎的系统评价[J].中国临床药理学与治疗学,2014,19(6):656-662.
[10]濮永杰,孔卫东,徐.不同剂量秋水仙碱治疗急性痛风疗效的Meta分析[J].中国药业,2015,24(16):21-24.
[11]黄敬群,孙文娟,王四旺,等.槲皮素对大鼠痛风性关节炎抗炎抗氧化活性研究[J].中国实验方剂学杂志,2012,18(2):169-173.
[12] CHUA LS. A review on plant-based rutin extraction methods and its pharmacological activities[J]. J Ethnopharmacol,2013,150(3):805.
[13]闫淑霞,李鲜,孙崇德,等.槲皮素及其糖苷衍生物降糖降脂活性研究进展[J].中国中药杂志,2015,40(23):4560-4567.