小儿健脾膏整方及其拆方对豚鼠离体胃肌、回肠平滑肌的作用比较
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摘要
目的观察比较小儿健脾膏整方及其拆方对豚鼠离体胃、回肠平滑肌的作用,进一步辨识方中促胃肠动力药效物质及其与整方的关系。方法运用生物信号采集系统记录小儿健脾膏整方及其拆方对正常豚鼠离体胃、回肠平滑肌收缩张力的影响。结果整方能显著促进胃、回肠平滑肌收缩。拆方中丁香、吴茱萸和山楂对胃肌收缩有促进作用,肉桂、白胡椒作用不显著。丁香和山楂对回肠平滑肌收缩有促进作用,且丁香>山楂>整方,吴茱萸则呈抑制作用,肉桂、白胡椒作用不显著。两两药物配伍对胃肌收缩有促进作用的组合为:丁香+肉桂、丁香+白胡椒、吴茱萸+肉桂、吴茱萸+白胡椒、山楂+肉桂。对回肠平滑肌收缩有促进作用的组合为:丁香+肉桂、丁香+白胡椒、山楂+肉桂;而吴茱萸+肉桂组、吴茱萸+白胡椒组则呈显著抑制作用。结论小儿健脾膏整方能显著促进豚鼠胃、回肠平滑肌收缩,其中,丁香和山楂为主要促胃肠动力单味药。
OBJECTIVE To evaluate the effect of the whole recipe of Xiaoer Jianpi cream and its disassembled prescription on the stomach muscle and ileum smooth muscle of guinea-pig, and the effect of ingredient on promoting gastrointestinal motility and its relationship with whole recipe is further studied. METHODS Biological functional system was used to record the effects of the whole recipe and its disassembled prescription on the contraction activity in stomach muscle and ileum smooth muscle of the normal guinea-pig. RESULTS The whole recipe had the effects on promoting contraction of ileum and stomach muscle. In its disassembled prescription, clove, evodia and hawthorn could increase the contractile amplitude in stomach muscle significantly, the effects of cinnamon, white pepper were not obvious. Clove and hawthorn could increase the contractile amplitude in ileum smooth muscle of guinea-pig significantly, clove > hawthorn > the whole recipe, the evodia showed an inhibitory effect, and the effect of cinnamon and white pepper was not significantly. The two part of the recipe by orthogonal method which had promoting effect on the gastric muscle were clove+cinnamon, clove+white pepper, evodia+ cinnamon, evodia+white pepper and hawthorn+cinnamon. The two part of the recipe by orthogonal method which had promoting effect on the ileum smooth muscle were clove + cinnamon, while clove + white pepper, hawthorn + cinnamon; evodia + cinnamon, evodia + white pepper showed significant inhibitory effect. CONCLUSION The Xiaoer Jianpi cream can promote the contractile amplitude in stomach muscle and ileum of guinea-pig significantly, clove and hawthorn are the main ingredient of promoting gastrointestinal motility.
OBJECTIVE To evaluate the effect of the whole recipe of Xiaoer Jianpi cream and its disassembled prescription on the stomach muscle and ileum smooth muscle of guinea-pig, and the effect of ingredient on promoting gastrointestinal motility and its relationship with whole recipe is further studied. METHODS Biological functional system was used to record the effects of the whole recipe and its disassembled prescription on the contraction activity in stomach muscle and ileum smooth muscle of the normal guinea-pig. RESULTS The whole recipe had the effects on promoting contraction of ileum and stomach muscle. In its disassembled prescription, clove, evodia and hawthorn could increase the contractile amplitude in stomach muscle significantly, the effects of cinnamon, white pepper were not obvious. Clove and hawthorn could increase the contractile amplitude in ileum smooth muscle of guinea-pig significantly, clove > hawthorn > the whole recipe, the evodia showed an inhibitory effect, and the effect of cinnamon and white pepper was not significantly. The two part of the recipe by orthogonal method which had promoting effect on the gastric muscle were clove+cinnamon, clove+white pepper, evodia+ cinnamon, evodia+white pepper and hawthorn+cinnamon. The two part of the recipe by orthogonal method which had promoting effect on the ileum smooth muscle were clove + cinnamon, while clove + white pepper, hawthorn + cinnamon; evodia + cinnamon, evodia + white pepper showed significant inhibitory effect. CONCLUSION The Xiaoer Jianpi cream can promote the contractile amplitude in stomach muscle and ileum of guinea-pig significantly, clove and hawthorn are the main ingredient of promoting gastrointestinal motility.
引文
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[4]房兆国.关于胃肠舒泰(颗粒)促进胃肠动力的临床研究[J].中国医药指南,2013(21):254-256.
[5]HONG T G,ZHONG H Y,HUANG G S,et al.Dose-effect relationship of superfine powder of Flos Caryophylli on isolated intestine of rats[J].Lishizhen Med Mater Med Res(时珍国医国药),2011(11):2647-2648.
[6]LOU L J,LUO J X,GAO Y.Overview of chemical compostion and pharmacological action of Grataegus pinnatifida Bunge[J].China Pharm(中国药业),2014,23(3):92-94.
[7]严春临,张季,薛贵平.中药吴茱萸药理作用研究概况[J].河北北方学院学报,2009,26(1):78.