葛花中总黄酮含量测定及提取工艺优化
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摘要
目的葛花中总黄酮含量的测定及提取工艺的优化。方法以总黄酮提取率为考察指标,采用冷凝回流、分光光度检测的方法,考察了单因素乙醇体积分数、物料比、提取时间对提取率的影响,采用3因素4水平[L16(43)]的正交实验,优化了提取工艺。结果最佳提取工艺条件为:乙醇体积分数为80%,物料比为1∶25(g∶m L),提取时间为1. 5h,提取4次,提取率为40. 54%。结论优化后的葛花总黄酮提取工艺合理可行,能为实际生产提供参考。
Objective To establish the determination method and optimization of extraction process of total flavonoids from Pueraria lobata. Methods Using the orthogonal experimental design method involving three factors and four levels [L16( 43) ],and taking the volume fraction,material ratio and extraction time of ethanol as factors and the extraction rate of total flavonoids as the index,both wavelength scanning methed and condensation reflux were used to optimize the above three factors and determine the optimum extraction process. Results The optimum extraction conditions were as follows: optimum volume fraction of ethanol was 80%,optimum raio of Pueraria to ethanol was 1∶ 25,the optimum reaction time was 1. 5 h. Under the optimum extraction conditions,the average extraction rate of toal flavonoids was 40. 54%. Conclusion The establishment of determination method and extraction process of tatol flavonoids from Pueraria lobata is reasonable and feasible,which could provide theoretical reference for the establishment of novel procedures for actual production.
Objective To establish the determination method and optimization of extraction process of total flavonoids from Pueraria lobata. Methods Using the orthogonal experimental design method involving three factors and four levels [L16( 43) ],and taking the volume fraction,material ratio and extraction time of ethanol as factors and the extraction rate of total flavonoids as the index,both wavelength scanning methed and condensation reflux were used to optimize the above three factors and determine the optimum extraction process. Results The optimum extraction conditions were as follows: optimum volume fraction of ethanol was 80%,optimum raio of Pueraria to ethanol was 1∶ 25,the optimum reaction time was 1. 5 h. Under the optimum extraction conditions,the average extraction rate of toal flavonoids was 40. 54%. Conclusion The establishment of determination method and extraction process of tatol flavonoids from Pueraria lobata is reasonable and feasible,which could provide theoretical reference for the establishment of novel procedures for actual production.
引文
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[2]马奋刚,张永萍,徐剑,等.葛花质量标准的研究[J].中成药,2018,40(8):1762
[3]王瑶.葛花黄酮部位质量控制及解酒保肝的药效学和作用机制研究[D].太原:山西中医药大学,2017
[4]徐庆国,谈金强,宋希林,等.葛花总黄酮对阿霉素所致中毒性心肌炎的保护作用研究[J].中国生化药物杂志,2014,34(7):27
[5]余薇,姚社,李璐,等.葛花总黄酮对乳鼠心肌细胞缺氧/复氧损伤的保护作用[J].广东医学,2010,31(14):1794
[6]彭常安,李媛媛.葛花总黄酮的抗氧化作用研究[J].安徽师范大学学报(自然科学版),2012,35(2):163
[7]欧阳昌汉,张瑞雪,叶涛,等.葛花总黄酮的含量测定及体外抗氧化作用研究[J].食品研究与开发,2012,33(11):57
[8]王金凤,赵楠,魏颖,等.正交试验法优选葛花异黄酮的提取工艺[J].药学实践杂志,2015,33(4):338
[9]吉莉莉,汪开毓,罗晓波,等.辣木叶总黄酮响应面法微波萃取工艺优化及其体外降糖效果观察[J].天然产物研究与开发,2015,27(6):979
[10]刘宇,俸丹,陈云,等.通江产辣木叶总黄酮的提取工艺优化及含量测定[J].中国兽药杂志,2018,52(8):50