邳州叶用嫁接银杏无性系选育
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摘要
根据银杏北方区域化嫁接试验,采用随机区组设计的方法,在江苏邳州市银杏种质资源圃嫁接6种无性系品种,测定比较不同品种银杏的光合指标、生理指标、内含物质量分数以及叶片次生代谢物质量分数,从中选择黄酮生产效益较高的无性系种类。结果表明:(1)各无性系可溶性糖质量分数基本在3.5%左右,相比当年生实生银杏苗质量分数较高;(2)各无性系可溶性蛋白质质量分数在1.6 mg/g左右,相比于1年生的银杏实生苗差别不明显;(3)根据无性系黄酮质量分数将6个无性系分为两类,13#、16#、27#代表的类别黄酮质量分数较低;叶丰、大花穗、佛指类为另一类,黄酮质量分数相对较高;(4)综合分析得出,佛指类为最适于邳州产区叶用银杏繁育无性系品种,其次为大花穗品种。
By random block design,six clones were grafted in the area of Pizhou City,Jiangsu Province. By measuring and comparing photosynthesis indicators,physiological index,inclusion mass fraction and leaf secondary metabolite mass fraction of different varieties of Ginkgo biloba,the higher production efficiency species were selected. The results show:( 1) The mass fraction of soluble sugar in each clone is about 3.5%,which is higher than that of the seed-borns in that year;( 2) The soluble protein contents of clones are about 1.6 mg/g. Compared with the annual Ginkgo seedlings,the difference is not obvious;( 3) According to clone flavonoid content,six clones were divided into two groups,one with low levels of Flavonoids includilng 13#,16#,27#,and the other is the kind of high levels of Flavonoids including YeF eng,DaHuaSui and FoZhi;( 4) FoZhi is the most suitable for the ginkgo breeding cultivar varieties in the Pizhou,followed by the DaHuaSui variety.
By random block design,six clones were grafted in the area of Pizhou City,Jiangsu Province. By measuring and comparing photosynthesis indicators,physiological index,inclusion mass fraction and leaf secondary metabolite mass fraction of different varieties of Ginkgo biloba,the higher production efficiency species were selected. The results show:( 1) The mass fraction of soluble sugar in each clone is about 3.5%,which is higher than that of the seed-borns in that year;( 2) The soluble protein contents of clones are about 1.6 mg/g. Compared with the annual Ginkgo seedlings,the difference is not obvious;( 3) According to clone flavonoid content,six clones were divided into two groups,one with low levels of Flavonoids includilng 13#,16#,27#,and the other is the kind of high levels of Flavonoids including YeF eng,DaHuaSui and FoZhi;( 4) FoZhi is the most suitable for the ginkgo breeding cultivar varieties in the Pizhou,followed by the DaHuaSui variety.
引文
[1]曹福亮.中国银杏志[M].北京:中国林业出版社,2007.
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[9]国靖.施肥对银杏用材林生长及生理的影响[D].南京:南京林业大学,2016.
[10]郁万文,刘新亮,曹福亮,等.不同银杏无性系叶药用成分差异及聚类分析[J].植物学报,2014,49(03):292-305.
[11]姚鑫.不同来源银杏叶资源化学研究[D].南京:南京中医药大学,2013.
[12]谢宝东.影响银杏叶有效成分黄酮、内酯含量相关因素的研究[D].山东:山东农业大学,2002.
[13]丁延朋.杂交金叶银杏一年生苗木性状研究[D].南京:南京林业大学,2017.
[14] ZHOU H,TANG W,ZENG J,et al. Screening of terpene lactones and flavonoid glycosides in Gingko biloba capsule by UPLCOrbitrap high resolution MS,with emphasis on isomer differentiation[J]. Journal of Food and Nutrition Research,2014,2(7):369-376.
[15]常丽.温度和土壤水分对银杏叶类黄酮合成的影响[D].南京:南京林业大学,2013.
[2] SINGH B,KAUR P,GOPICHAND R,et al. Biology and chemistry of Ginkgo biloba[J]. Fitoterapia,2008,79(6):401-418.
[3]沈迎新,丁之恩.银杏的绿化功能研究[J].经济林研究.1999,17(4):19-21.
[4]祝遵凌,曹福亮,孟凡军,等.黄叶银杏新品种‘万年金’区域化试验[J].中南林业科技大学学报,2012,32(12):125-131.
[5]郁万文.银杏抗寒机理及种质资源抗寒性评定的初步研究[D].南京:南京林业大学,2008:125-126.
[6]曹晓霞,郭建斌,杨晓菲,等.不同浓度叶面肥处理对核桃瞬时水分利用效率的影响[J].西北林学院学报,2011,26(3):26-29.
[7]潘瑞炽.植物生理学[M].北京:高等教育出版社,2004.
[8]王扬,房荣春,林明明,等.银杏叶片生长发育过程中生理生化指标动态变化[J].江苏农业科学,2011,39(4):155-158.
[9]国靖.施肥对银杏用材林生长及生理的影响[D].南京:南京林业大学,2016.
[10]郁万文,刘新亮,曹福亮,等.不同银杏无性系叶药用成分差异及聚类分析[J].植物学报,2014,49(03):292-305.
[11]姚鑫.不同来源银杏叶资源化学研究[D].南京:南京中医药大学,2013.
[12]谢宝东.影响银杏叶有效成分黄酮、内酯含量相关因素的研究[D].山东:山东农业大学,2002.
[13]丁延朋.杂交金叶银杏一年生苗木性状研究[D].南京:南京林业大学,2017.
[14] ZHOU H,TANG W,ZENG J,et al. Screening of terpene lactones and flavonoid glycosides in Gingko biloba capsule by UPLCOrbitrap high resolution MS,with emphasis on isomer differentiation[J]. Journal of Food and Nutrition Research,2014,2(7):369-376.
[15]常丽.温度和土壤水分对银杏叶类黄酮合成的影响[D].南京:南京林业大学,2013.