摘要
新城疫病毒样颗粒已开发成一种高效递送外源蛋白的载体平台。本研究在小鼠模型中开展了嵌合布鲁菌BCSP31病毒样颗粒(BCSP31-cVLPs)免疫原性及其保护效力评价。体内试验数据表明,BCSP31-cVLPs能有效激活脾脏中树突状细胞,进而促进初始型CD3~+CD4~+ T的活化。ELISA法检测小鼠血清结果表明,BCSP31-cVLPs可刺激机体产生针对重组BCSP31蛋白的特异性抗体水平,且呈剂量依赖性。流式细胞术检测结果显示,BCSP31-cVLPs可激活T细胞并使其分化。攻毒结果表明,BCSP31-cVLPs具有与疫苗株M5相当的免疫保护效力。本研究为新型布鲁菌疫苗的研制提供了数据支撑,并扩展了新城疫病毒样颗粒疫苗载体平台的应用。
Newcastle disease virus-like particles have been developed as a vector platform for efficient delivery of foreign proteins.In this study,the immunogenicity and protective efficacy of chimeric Brucella BCSP31 virus-like particles(BCSP31-cVLPs) were evaluated in a mouse model.In vivo data indicate that BCSP31-cVLPs can effectively activate dendritic cells in the spleen and further promote the activation of the original CD3~+CD4~+ T.ELISA results showed that BCSP31-cVLPs stimulated the body to produce specific antibody against recombinant BCSP31 protein in a dose-dependent manner.Flow cytometry results showed that BCSP31-cVLPs can effectively activate T lymphocytes and induce differentiation.The results of the challenge showed that BCSP31-cVLPs have comparable immunoprotective efficacy to vaccine strain M5.In summary,this study provides data support for the development of a new Brucella vaccine and extends the application of the Newcastle disease virus-like particle vaccine vector platform.
引文
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