六味地黄丸对D-半乳糖致衰老大鼠抗氧化功能影响的实验研究
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摘要
目的:观察六味地黄丸丸对D-半乳糖致衰老大鼠抗氧化能力的影响,以探讨其延缓衰老的机理。方法:建立D-半乳糖致亚急性衰老大鼠模型,实验分为正常组、模型组、维生素E对照组和六味地黄丸4组,每组10只;分组给药8周后,测定各组大鼠肾组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;血一氧化氮(NO)、一氧化氮合成酶(NOS)含量。结果:与正常组比较,模型组肾组织SOD活性显著降低,MDA含量显著增高;予以六味地黄丸干预后,衰老大鼠肾组织SOD活性升高,MDA含量下降,血NO、NOS的含量也降低。结论:六味地黄丸具有抗氧化作用。
Objective: To research the influence of Liuwei Dihuang Pill on antioxidantive action in aging rats induced by D-galactose,and explore the machanism of antioxidantive action. Methods: Rats was injected with D-galactose subcutaneously to establish the aging models,experiment was divided into 4 groups,normal,model,positive control and Liuwei Dihuang Pill groups with each 10 rats,each group rats were given the corresponding saline or medicines for 8 weeks,then determining the SOD activity and MDA content in rats' renal tissue,blood NO and NOS contents. Results: Compared with normal group,the activity of SOD decreased significantly and the content of MDA increased in model group,SOD activity of renal tissue in Liuwei Dihuang Pill group was enhanced and MDA content was reduced,blood NO and NOS contents were lowered. Conclusion: Liuwei Dihuang Pill can delay senility by antioxidant.
Objective: To research the influence of Liuwei Dihuang Pill on antioxidantive action in aging rats induced by D-galactose,and explore the machanism of antioxidantive action. Methods: Rats was injected with D-galactose subcutaneously to establish the aging models,experiment was divided into 4 groups,normal,model,positive control and Liuwei Dihuang Pill groups with each 10 rats,each group rats were given the corresponding saline or medicines for 8 weeks,then determining the SOD activity and MDA content in rats' renal tissue,blood NO and NOS contents. Results: Compared with normal group,the activity of SOD decreased significantly and the content of MDA increased in model group,SOD activity of renal tissue in Liuwei Dihuang Pill group was enhanced and MDA content was reduced,blood NO and NOS contents were lowered. Conclusion: Liuwei Dihuang Pill can delay senility by antioxidant.
引文
[1]张常娥,魏伟,刘英华,等. D-半乳糖亚急性衰老大鼠模型的建立及评价[J].中国老年学杂志,2012,32(2):742-745.
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[6]王树鹏,刘书宇.龟鹿二仙胶颗粒对D-半乳糖所致衰老大鼠抗氧化能力的影响[J].中药药理与临床,2011,27(1):1-2.
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[8]王进,杜波,单爱军.内皮型一氧化氮合酶基因多态性对一氧化氮水平的影响[J].医学综述,2014,20(4):580-583.
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[10]边宁,龚博君,郭军,等.一氧化氮/诱导型一氧化氮合酶在动脉粥样硬化过程中的作用[J].中国病理生理杂志,2014,30(3):414-418.
[11]石少慧,王怀颖,张晶晶,等.六味地黄汤对衰老小鼠肾脏Caspase-3表达及活性的影响[J].中国老年学杂志,2013,33(2):349-350.
[12]孙琳林,王书惠,卢林,等.左归丸与六味地黄丸对衰老大鼠抗氧化能力及海马区超微结构影响的比较研究[J].中医药学报,2012,40(3):140-142.
[2]李罗德,叶俊玲,晏子友.六味地黄丸(汤)治疗肾脏病临床研究概况[J].江西中医药,2012,43(2):69-71.
[3]朱瑄.六味地黄丸的药理研究新进展[J].中医临床研究,2010,2(24):23.
[4]李全斌.六味地黄方研究进展[J].辽宁中医药大学学报,2013,15(1):217-219.
[5]邢秋娟,施杞,王拥军. D一半乳糖诱导衰老动物模型的机制及其在中医药方面的应用[J].上海中医药大学学报,2010,24(3):93-98.
[6]王树鹏,刘书宇.龟鹿二仙胶颗粒对D-半乳糖所致衰老大鼠抗氧化能力的影响[J].中药药理与临床,2011,27(1):1-2.
[7]杨松鹤,董晓彤,杨国军. D-半乳糖衰老大鼠抗氧化能力及血脂含量的观测[J].承德医学院学报,2005,22(2):99-101.
[8]王进,杜波,单爱军.内皮型一氧化氮合酶基因多态性对一氧化氮水平的影响[J].医学综述,2014,20(4):580-583.
[9]王进,杜波,单爱军.内皮型一氧化氮合酶基因多态性对一氧化氮水平的影响[J].医学综述,2014,20(4):580-583.
[10]边宁,龚博君,郭军,等.一氧化氮/诱导型一氧化氮合酶在动脉粥样硬化过程中的作用[J].中国病理生理杂志,2014,30(3):414-418.
[11]石少慧,王怀颖,张晶晶,等.六味地黄汤对衰老小鼠肾脏Caspase-3表达及活性的影响[J].中国老年学杂志,2013,33(2):349-350.
[12]孙琳林,王书惠,卢林,等.左归丸与六味地黄丸对衰老大鼠抗氧化能力及海马区超微结构影响的比较研究[J].中医药学报,2012,40(3):140-142.