响应面法优化刺楸叶单宁的提取工艺及其抗氧化
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摘要
对刺楸叶中单宁的提取工艺进行优化,利用定性试验鉴定单宁的类型,并对其抗氧化活性进行测定。结果表明,在单因素试验基础上,通过响应面分析法确定刺楸叶单宁提取的最佳工艺为:乙醇体积分数57%,提取时间0.9 h,提取温度61℃。此条件下单宁得率为4.05%,定性试验鉴定刺楸叶单宁为缩合类单宁,刺楸叶单宁提取物清除DPPH、ABTS自由基的IC50值分别为0.38 mg/mL和0.22 mg/mL。
The extraction process of tannins from Kalopanax septemlobus was optimized and the type of tannins was identified by qualitative test, and then their antioxidant activity was evaluated. The results showed that the optimum condition for tannins from Kalopanax septemlobus by response surface method was found to be with 57% ethanol for 0.9 h with temperature 61 ℃ on the basis of single factor tests. Under such condition, the yield of tannins was 4.05%. The type of tannins was identified as condensed tannins by qualitative test and their DPPH and ABTS free radical scavenging activity IC50 values were 0.38 mg/mL and 0.22 mg/mL, respectively.
The extraction process of tannins from Kalopanax septemlobus was optimized and the type of tannins was identified by qualitative test, and then their antioxidant activity was evaluated. The results showed that the optimum condition for tannins from Kalopanax septemlobus by response surface method was found to be with 57% ethanol for 0.9 h with temperature 61 ℃ on the basis of single factor tests. Under such condition, the yield of tannins was 4.05%. The type of tannins was identified as condensed tannins by qualitative test and their DPPH and ABTS free radical scavenging activity IC50 values were 0.38 mg/mL and 0.22 mg/mL, respectively.
引文
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[20]王川,李光辉.葡萄籽中单宁的提取研究[J].食品研究与开发,2006,27(10):25-27.
[21]方超,陈亚君,毛卉,等.基于杨梅单宁制备Pt/SiO2-C催化剂及其对肉桂醛选择性催化加氢性能[J].高等学校化学学报,2015,36(1):124-130.
[22]王性炎,孙波,梁志荣,等.元宝枫栲胶的制取、分析及应用[J].陕西科技大学学报,1994(3):60-65.
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[25]石碧,狄莹.植物多酚[M].北京:科学出版社,2000:5-17.
[26]杨琼琼,李东,屈恋,等.涩柿树皮单宁的纯化及抗氧化活性[J].食品科学,2016,37(7):50-55.
[2]LEE E B,LI D W,HYUN J E,et al.Anti-inflammatory activity of methanol extract of Kalopanax pictus bark and its fractions[J].Journal of Ethnopharmacology,2001,77(2):197-201.
[3]南京中医药大学.中药大辞典[M].上海:上海科学技术出版社,2006:1848-1849.
[4]KIM Y K,KIM R G,PARK S J,et al.In vitro antiinflammatory activity of kalopanaxsaponin A isolated from Kalopanax pictus in murine macrophage RAW 264.7 cells[J].Biological&Pharmaceutical Bulletin,2002,25(4):472-476.
[5]杨月,唐祖年,韦玉先,等.广西刺楸茎皮中降血糖活性成分的研究[J].武汉大学学报(医学版),2008,29(6):759-762.
[6]汪有科,盛义保,陈书文,等.我国野生木本蔬菜资源开发利用现状及其发展前景[J].西北林学院学报,2001,16(3):37-41.
[7]李义,吴岩,石博,等.刺楸播种育苗技术[J].林业机械与木工设备,2011,39(9):54-55.
[8]刘盛全,江泽慧.刺楸木材应拉木材性研究[J].林业科学,1996,32(5):470-475.
[9]PANOV D A,GRISHKOVETS V I,KACHALA V V,et al.Triterpene glycosides from Kalopanax septemlobum.VII.Minor glycosides from stems of Kalopanax septemlobum,var.maximowiczii,and Kalopanax septemlobum,var.typicum[J].Chemistry of Natural Compounds,2006,42(1):61-66.
[10]YIN J Y,MENG Q,WANG E S.Septemloside III:Anonasaccharide saponin from the bark of Kalopanax septemlobus(Thunb)Koidz[J].中国化学快报(英文版),2005,16(1):53-56.
[11]朱玮,贾夏,张鞍灵,等.刺楸属植物化学成分及生物活性研究进展[J].西北林学院学报,2004,19(3):119-124.
[12]张参战,李志洲.植物多酚在化妆品中的应用机理及开发[J].安康学院学报,2004,16(1):87-89.
[13]SHIN M,RYU J H,PARK J P,et al.DNA/tannic acid hybrid gel exhibiting biodegradability,extensibility,tissue adhesiveness,and hemostatic ability[J].Advanced Functional Materials,2015,25(8):1270-1278.
[14]傅长明,黄科林,王则奋,等.植物单宁的性质及应用[J].企业科技与发展,2010(22):7-60.
[15]ZHANG L H,LI L L,ZHANG Y H,et al.In vitro antioxidant activities of fruits and leave of pomegranate[C].2006.
[16]WANG Q,LI Y,SUN F,et al.Tannins improve dough mixing properties through affecting physicochemical and structural properties of wheat gluten proteins[J].Food Research International,2015,69(1):64-71.
[17]LANSKY E P,NEWMAN R A.Punica granatum(pomegranate)and its potential for prevention and treatment of inflammation and cancer[J].Journal of Ethnopharmacology,2007,109(2):177-206.
[18]PARK C,JEONG J S,JEONG J W,et al.Ethanol extract of Kalopanax septemlobus leaf inhibits HepG2 human hepatocellular carcinoma cell proliferation via inducing cell cycle arrest at G1 phase[J].Asian Pacific Journal of Tropical Medicine,2016,9(4):344-350.
[19]杨连利,李仲瑾,苏秀霞,等.元宝枫单宁类化合物的提取研究[J].皮革与化工,2004,21(5):1-3.
[20]王川,李光辉.葡萄籽中单宁的提取研究[J].食品研究与开发,2006,27(10):25-27.
[21]方超,陈亚君,毛卉,等.基于杨梅单宁制备Pt/SiO2-C催化剂及其对肉桂醛选择性催化加氢性能[J].高等学校化学学报,2015,36(1):124-130.
[22]王性炎,孙波,梁志荣,等.元宝枫栲胶的制取、分析及应用[J].陕西科技大学学报,1994(3):60-65.
[23]BRAND-WILLIAMS W,CUVELIER M E,BERSET C.Use of a free radical method to evaluate antioxidant activity[J].Lwt Food Sci Technol,1995,28(1):25-30.
[24]王华.杏鲍菇中三萜类化合物的抗氧化、抗肿瘤活性的研究[D].天津:天津大学,2012.
[25]石碧,狄莹.植物多酚[M].北京:科学出版社,2000:5-17.
[26]杨琼琼,李东,屈恋,等.涩柿树皮单宁的纯化及抗氧化活性[J].食品科学,2016,37(7):50-55.