牙周炎差异表达基因的筛选、信号通路预测:基于GEO数据库生物信息学分析
摘要
目的探讨牙周炎差异表达基因及其在牙周炎发病机制中的作用和相关信号通路。方法计算机系统检索GEO数据库中关于牙周炎相关基因表达谱芯片数据集,采用GEOR软件分析差异表达基因,绘制火山图。对筛选出的差异表达基因进行基因本体论(GO)和京都基因与基因组百科全书(KEEG)分析,预测其可能功能及信号通路。采用蛋白-蛋白相互作用数据库(STRING)筛选出的差异表达基因编码蛋白间的相互作用关系。并用Cytohubba软件鉴定信号通路关键基因。结果选取了GSE79705数据集为研究对象,共筛选出差异表达基因40个;其中上调表达38个,下调表达2个。功能富集分析显示,上述差异表达基因主要富集于对脂多糖的反应、免疫球蛋白介导免疫反应的免疫球蛋白产生、MHCⅡ类受体活性、趋化因子活性等。信号通路主要富集于炎症反应、免疫应答和白细胞趋化。40个差异表达基因中,CXCL8为信号通路的关键基因。结论牙周炎患者齿龈沟液中存在差异表达基因谱,且差异表达基因大多富集于炎性反应生物学功能及信号通路, CXCL8在慢性牙周炎发病中可能发挥重要关键基因作用。
引文
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[4] Dennis G,Sherman BT,Hosack DA,et al. DAVID:Database for Annotation, Visualization, and Integrated Discovery[J]. Genome Biol,2003,4(5):P3.
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[8] Sima C,Viniegra A,Glogauer M. Macrophage immunomodulation inchronic osteolytic diseases-the case of periodontitis[J]. J Leukoc Biol, 2019,105(3):473-487.
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