基于电子眼技术的穿心莲质量评价
|
摘要
目的:采用电子眼(视觉分析仪)分析技术,基于穿心莲药材粉末颜色,探讨电子眼用于穿心莲药材质量评价的适用性,并对不同商品规格的穿心莲药材质量进行评价。方法:收集50批次不同商品规格(茎、叶、地上部分)的穿心莲药材,采用高效液相色谱法(HPLC)建立穿心莲药材中4个二萜内酯类有效成分的分析方法,并对所有样品进行分析;与此同时采用电子眼对样品颜色进行检测。在此基础上,采用主成分分析(PCA)及Pearson相关分析对电子眼数据和含量测定结果进行分析,考察电子眼对不同规格穿心莲药材的区分能力,以及色度空间系统参数(L*,a*,b*)与有效成分的相关性。结果:穿心莲含量测定结果显示不同规格的穿心莲药材中,叶中的二萜内酯类成分含量最高,其次为地上部分(茎、叶混合),茎中含量最低;电子眼采集数据的PCA结果将穿心莲药材颜色分为2部分,即茎部分和叶、地上部分,说明电子眼可用于区分穿心莲药材质量的优劣。相关性分析结果显示穿心莲内酯,脱水穿心莲内酯,14-去氧穿心莲内酯,新穿心莲内酯及4个成分的总含量均与L*(明度值)呈极显著负相关(P <0. 01),并且所测结果中不符合2015年版《中国药典》含量测定下限规定的样品其L*均≥69. 5,符合规定的样本90%以上的L*均<69. 5。结论:电子眼分析技术可用于穿心莲药材的质量评价,为穿心莲药材的质量评价提供了新方法及新思路。
Objective: Taking electronic-eye(visual analyzer) technique,based on the powder color of Andrographis Herba, to investigate the applicability of electronic-eye technique and evaluate the quality of Andrographis Herba with different commercial specifications. Method: HPLC was employed to determine contents of andrographolide, dehydroandrographolide, 14-deoxyandrographolide, neoandrographolide in 50 batches of Andrographis Herba with different commercial specifications(stems,leaves and aerial parts). Color of these samples were measured by electronic-eye technique. The data were analyzed by principal component analysis(PCA) and Pearson correlation analysis. The ability of electronic-eye to distinguish the different commercial specifications of Andrographis Herba was investigated and the correlation of chroma space system parameters(L*,a*,b*) with active components was investigated. Result: There was remarkable difference in contents of 4 diterpenoids in Andrographis Herba from different parts,their contents in leaves was the highest,followed by the aerial parts(mixture of stems and leaves),and their contents in stems was the lowest. The results of PCA was divided into two classes,namely the stem part,leaf and aerial parts,indicating that electronic-eye could be used to distinguish the quality of Andrographis Herba. The correlation results showed that there were significant negative correlation(P < 0. 01) between L*(lightness value) and the contents of andrographolide,dehydroandrographolide, 14-deoxyandrographolide, neoandrographolide and the total content of these 4 components. In addition,L*of samples that did not conform to the lower limit of determination in the 2015 edition of Chinese Pharmacopeia was ≥ 69. 5, and the L*of more than 90% of the samples in accordance with the requirements was < 69. 5. Conclusion: Electronic-eye technique provides a new method and idea for the quality evaluation of Andrographis Herba.
Objective: Taking electronic-eye(visual analyzer) technique,based on the powder color of Andrographis Herba, to investigate the applicability of electronic-eye technique and evaluate the quality of Andrographis Herba with different commercial specifications. Method: HPLC was employed to determine contents of andrographolide, dehydroandrographolide, 14-deoxyandrographolide, neoandrographolide in 50 batches of Andrographis Herba with different commercial specifications(stems,leaves and aerial parts). Color of these samples were measured by electronic-eye technique. The data were analyzed by principal component analysis(PCA) and Pearson correlation analysis. The ability of electronic-eye to distinguish the different commercial specifications of Andrographis Herba was investigated and the correlation of chroma space system parameters(L*,a*,b*) with active components was investigated. Result: There was remarkable difference in contents of 4 diterpenoids in Andrographis Herba from different parts,their contents in leaves was the highest,followed by the aerial parts(mixture of stems and leaves),and their contents in stems was the lowest. The results of PCA was divided into two classes,namely the stem part,leaf and aerial parts,indicating that electronic-eye could be used to distinguish the quality of Andrographis Herba. The correlation results showed that there were significant negative correlation(P < 0. 01) between L*(lightness value) and the contents of andrographolide,dehydroandrographolide, 14-deoxyandrographolide, neoandrographolide and the total content of these 4 components. In addition,L*of samples that did not conform to the lower limit of determination in the 2015 edition of Chinese Pharmacopeia was ≥ 69. 5, and the L*of more than 90% of the samples in accordance with the requirements was < 69. 5. Conclusion: Electronic-eye technique provides a new method and idea for the quality evaluation of Andrographis Herba.
引文
[1]国家药典委员会.中国人民共和国药典.一部[M].北京:中国医药科技出版社,2015:268-269.
[2]闫婕,卫莹芳,胡慧玲,等.穿心莲药用植物资源调查[J].时珍国医国药,2013,24(8):1997-1999.
[3]蒋珍藕.穿心莲属植物化学成分研究进展[J].中成药,2011,33(8):1382-1388.
[4] TAN W S D,LIAO W,ZHOU S,et al. Is there a future for andrographolide to be an anti-inflammatory drug?Deciphering its major mechanisms of action[J].Biochem Pharmacol,2017,139:71-81.
[5] Hussain R M,Razak Z N R A,Saad W M M,et al.Mechanism of antagonistic effects of Andrographis paniculata methanolic extract against Staphylococcus aureus[J]. Asian Pac J Trop Med,2017,10(7):685-695.
[6] Kumar R A,Sridevi K,Kumar N V,et al. Anticancer and immunostimulatory compounds from Andrographis paniculata[J]. J Ethnopharmacol,2004,92(2/3):291-295.
[7] Awang K,Abdullah N H,Hadi A H A,et al.Cardiovascular activity of labdane diterpenes from Andrographis paniculata in isolated rat hearts[J]. J Biomed Biotechnol,2012,doi:10. 1155/2012/876458.
[8] Nugroho A E,Ardrie M,Warditiani N K,et al.Antidiabetic and antihiperlipidemic effect of Andrographis paniculata(Burm. f.)Nees and andrographolide in high-fructose-fat-fed rats[J]. Indian J Pharmacol,2012,44(3):377-381.
[9] SHI G J,ZHANG Z J,ZHANG R,et al. Protective effect of andrographolide against concanavalin A-induced liver injury[J]. Naunyn Schmiedebergs Arch Pharmacol,2012,385(1):69-79.
[10]刘芃.中药鉴定学[M].北京:中医古籍出版社,2003:323.
[11]郭换,刘飞,梅国荣,等.色度分析花椒黄酮类成分含量与颜色值的相关性[J].中国实验方剂学杂志,2017,23(6):91-97.
[12]马婷婷,龚慕辛,王智民,等.甘草色泽与有效成分含量的相关性研究[J].中国中药杂志,2017,42(19):3776-3785.
[13]周凯岚.穿心莲和旱芹的化学成分研究[D].沈阳:沈阳药科大学,2008.
[14]徐曼菲,吴志生,刘晓娜,等.从辨色论质谈中药质量评价方法[J].中国中药杂志,2016,41(2):177-181.
[15]李旻,陈美君,潘欢欢,等.基于颜色客观化的陈皮药材鉴别[J].中国实验方剂学杂志,2016,22(18):31-34.
[2]闫婕,卫莹芳,胡慧玲,等.穿心莲药用植物资源调查[J].时珍国医国药,2013,24(8):1997-1999.
[3]蒋珍藕.穿心莲属植物化学成分研究进展[J].中成药,2011,33(8):1382-1388.
[4] TAN W S D,LIAO W,ZHOU S,et al. Is there a future for andrographolide to be an anti-inflammatory drug?Deciphering its major mechanisms of action[J].Biochem Pharmacol,2017,139:71-81.
[5] Hussain R M,Razak Z N R A,Saad W M M,et al.Mechanism of antagonistic effects of Andrographis paniculata methanolic extract against Staphylococcus aureus[J]. Asian Pac J Trop Med,2017,10(7):685-695.
[6] Kumar R A,Sridevi K,Kumar N V,et al. Anticancer and immunostimulatory compounds from Andrographis paniculata[J]. J Ethnopharmacol,2004,92(2/3):291-295.
[7] Awang K,Abdullah N H,Hadi A H A,et al.Cardiovascular activity of labdane diterpenes from Andrographis paniculata in isolated rat hearts[J]. J Biomed Biotechnol,2012,doi:10. 1155/2012/876458.
[8] Nugroho A E,Ardrie M,Warditiani N K,et al.Antidiabetic and antihiperlipidemic effect of Andrographis paniculata(Burm. f.)Nees and andrographolide in high-fructose-fat-fed rats[J]. Indian J Pharmacol,2012,44(3):377-381.
[9] SHI G J,ZHANG Z J,ZHANG R,et al. Protective effect of andrographolide against concanavalin A-induced liver injury[J]. Naunyn Schmiedebergs Arch Pharmacol,2012,385(1):69-79.
[10]刘芃.中药鉴定学[M].北京:中医古籍出版社,2003:323.
[11]郭换,刘飞,梅国荣,等.色度分析花椒黄酮类成分含量与颜色值的相关性[J].中国实验方剂学杂志,2017,23(6):91-97.
[12]马婷婷,龚慕辛,王智民,等.甘草色泽与有效成分含量的相关性研究[J].中国中药杂志,2017,42(19):3776-3785.
[13]周凯岚.穿心莲和旱芹的化学成分研究[D].沈阳:沈阳药科大学,2008.
[14]徐曼菲,吴志生,刘晓娜,等.从辨色论质谈中药质量评价方法[J].中国中药杂志,2016,41(2):177-181.
[15]李旻,陈美君,潘欢欢,等.基于颜色客观化的陈皮药材鉴别[J].中国实验方剂学杂志,2016,22(18):31-34.