抑制miR-210的表达对鼻咽癌放射抵抗细胞敏感性的影响
摘要
目的探讨抑制miR-210的表达对鼻咽癌放射抵抗细胞(CNE-2R)敏感性的影响。方法对人鼻咽癌细胞株(CNE-2)进行不同梯度的X射线反复辐射诱导,建立CNE-2R细胞株;用双向凝胶电泳结合质谱分析法检测2种细胞株蛋白的表达;用miR-210抑制慢病毒载体LV-hsa-miR-210-inhibition感染CNE-2R细胞生成miR-210抑制细胞(I-210);RT-qPCR检测I-210中miR-210表达量;细胞计数试剂盒(CCK-8)技术检测I-210与CNE-2R细胞生长能力;流式细胞仪检测细胞凋亡和细胞周期;对CNE-2R和I-210分别给予不同剂量的X射线照射,培养2 w后,利用克隆成体实验检测CNE-2R与I-210计算放射增敏比,检测细胞放射敏感性。结果 CNE-2与其诱导后生成的CNE-2R比较,有36个蛋白表达存在差异。I-200的miR-210表达显著降低,细胞存活率显著降低,细胞凋亡比例显著上升,G2/M期比例显著增加,而处于S期的细胞比例显著减少,放射增敏比上升(P<0.05)。结论抑制miR-210表达,可增强鼻咽癌放抗细胞的敏感性。
引文
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19 Dong Y,Yu J,Ng SS,et al.MicroRNA dysregulation as a prognostic biomarker in colorectal cancer[J].Cancer Manag Res,2014;6(4):405-22.
2 郑荣辉,孔彩琴,田允鸿.广东鼻咽癌早期发现与误诊现况临床定量研究[J].实用医学杂志,2016;32(12):1952-5.
3 Pai T,Nair N,Pantvaidya G,et al.Metastatic nasopharyngeal carcinoma presenting as an isolated breast mass:a diagnostic pitfall and a review of literature[J].Indian J Pathol Microbiol,2017;60(1):119-21.
4 Halvorsen AR,Kristensen G,Embleton A,et al.Evaluation of prognostic and predictive significance of circulating micrornas in ovarian cancer patients[J].Dis Markers,2017;2017;3098542.
5 Botta C,Cucè M,Caracciolo D,et al.Immunomodulatory activity of microRNAs:potential implications for multiple myeloma treatment[J].Curr Cancer Drug Targets,2017;17(9):819-38.
6 Yang X,Chen Y,Chen L.The versatile role of microRNA-30a in human cancer[J].Cell Physiol Biochem,2017;41(4):1616-32.
7 Sun R,Liu Z,Tong D,et al.MiR-491-5p,mediated by foxi1,functions as a tumor suppressor by targeting Wnt3a/β-catenin signaling in the development of gastric cancer[J].Cell Death Dis,2017;8(3):185-91.
8 Yuan W,Xiaoyun H,Hai Feng Q,et al.MicroRNA-218 enhances the radio-sensitivity of human cervical cancer via promoting radiation induced apoptosis[J].Int J Med Sci,2014;11(7):691-4.
9 郭亚,朱小东,曲颂,等.沉默STAT1基因增强放射抗拒鼻咽癌CNE-2R细胞放射敏感性[J].中华放射医学与防护杂志,2015;35(5):334-8.
10 Liu C,Tang X.Down regulation of microRNA-210 inhibits osteosarcoma growth in vitro and in vivo[J].Mol Med Rep,2015;12(3):3674-80.
11 方茜,曲爱林,张欣.血清miR-210在结直肠癌患者血清中的表达及临床意义[J].山东大学学报:医学版,2015;53(6):77-81.
12 Yu P,Fan S,Huang L,et al.MIR210 as a potential molecular target to block invasion and metastasis of gastric cancer[J].Med Hypotheses,2015;84(3):209-12.
13 葛甜甜,梁勇,付蓉,等.淋巴瘤患者血浆中miR-21、miR-155、miR-210的表达及临床意义[J].中国实验血液学杂志,2012;20(2):305-9.
14 楼梦.HIF-1α和miR-210在宫颈鳞癌中的表达及其相关性[J].重庆医科大学学报,2015;40(4):604-7.
15 Wang H,Flach H,Onizawa M,et al.Negative regulation of Hif1a expression and TH17 differentiation by the hypoxia-regulated microRNA miR-210[J].Nat Immunel,2014;15(4):393-401.
16 陈慧,莫淋.miR-210高表达与乳腺癌预后关系的meta分析[J].南京医科大学学报(自然科学版),2016;26(2):239-44.
17 黄建国,王勇强,董雅璐.低氧诱导U251细胞中miRNA-210的表达及对肿瘤转移的影响[J].肿瘤防治研究,2013;40(10):954-7.
18 黎博一,赵文思,张露,等.下调miR210增加放射抗拒鼻咽癌细胞系CNE-2R的放射敏感性[J].基础医学与临床,2016;36(9):1227-31.
19 Dong Y,Yu J,Ng SS,et al.MicroRNA dysregulation as a prognostic biomarker in colorectal cancer[J].Cancer Manag Res,2014;6(4):405-22.