电针对慢性疲劳综合征大鼠脑组织转化生长因子-β_1基因转录和蛋白表达的影响
|
摘要
目的:探讨电针治疗对慢性疲劳综合征(CFS)模型大鼠脑组织中TGF-β_1基因转录及蛋白表达的影响。方法:雄性SD大鼠48只,随机分为正常组、模型组、束缚组和针刺组,各12只。模型组采用慢性复合应激因素制备CFS模型;束缚组仅给予与针刺相同时间的捆绑;针刺组给予百会、额区、顶区电针治疗;共15 d。RT-PCR技术检测各组TGF-β_1m RNA的转录水平,Western blot技术检测各组TGF-β_1蛋白表达水平。结果:模型组和束缚组大鼠海马和下丘脑中TGF-β_1m RNA转录水平显著高于正常组(P<0.01),针刺组与正常组差异无统计学意义(P>0.05);针刺组海马和下丘脑中TGF-β_1m RNA转录水平显著低于模型组(P<0.01),束缚组和模型组差异无统计学意义(P>0.05)。正常组中TGF-β_1蛋白表达显著低于其他3组(P<0.01);针刺组大鼠海马和下丘脑中TGF-β_1蛋白表达水平明显低于模型组(P<0.01),束缚组和模型组差异无统计学意义(P>0.05)。结论:电针能够降低CFS模型大鼠海马与下丘脑中TGF-β_1m RNA转录水平和TGF-β_1蛋白表达水平。
Objective: To study the effect of electric acupuncture on the transforming growth factor(TGF)-β_1 signaling pathways of the chronic fatigue syndrome(CFS) rat model. Methods: Forty-eight SD rats were randomly assigned to the normal group, model group, bound group, and acupuncture group with 12 rats per group. The CFS rat model was established by applying multiple chronic stress factors. The bound group rats were subject to binding for an equal duration of time as that of the acupuncture treatment applied to the acupuncture group rats. Acupuncture group rats were treated for 15 days by electric acupuncture to the Baihui acupoint,frontal lobe area, and parietal lobe area. TGF-β_1 m RNA transcription was tested by RT-PCR, and TGF-β_1 expression was assessed by Western blot. Results: Model group and bound group rats showed significantly increased TGF-β_1 m RNA transcription levels in the hippocampus and hypothalamus compared to normal group rats(P<0.01); there was no significant difference between the acupuncture group and normal group(P>0.05).The acupuncture group showed significantly decreased TGF-β_1 m RNA transcription levels compared to the model group(P<0.01) while the bound group and model group showed no significant difference(P>0.05). The normal group displayed lower TGF-β_1 expression compared to the other 3 groups(P<0.01). Acupuncture group rats showed significantly decreased TGF-β_1 expression levels in the hippocampus and hypothalamus compared to model group rats(P<0.01); the bound group and model group showed no significant difference(P>0.05).Conclusion: Electric acupuncture can reduce TGF-β_1 m RNA transcription levels and TGF-β_1 protein expression in the hippocampus and hypothalamus of CFS model rats.
Objective: To study the effect of electric acupuncture on the transforming growth factor(TGF)-β_1 signaling pathways of the chronic fatigue syndrome(CFS) rat model. Methods: Forty-eight SD rats were randomly assigned to the normal group, model group, bound group, and acupuncture group with 12 rats per group. The CFS rat model was established by applying multiple chronic stress factors. The bound group rats were subject to binding for an equal duration of time as that of the acupuncture treatment applied to the acupuncture group rats. Acupuncture group rats were treated for 15 days by electric acupuncture to the Baihui acupoint,frontal lobe area, and parietal lobe area. TGF-β_1 m RNA transcription was tested by RT-PCR, and TGF-β_1 expression was assessed by Western blot. Results: Model group and bound group rats showed significantly increased TGF-β_1 m RNA transcription levels in the hippocampus and hypothalamus compared to normal group rats(P<0.01); there was no significant difference between the acupuncture group and normal group(P>0.05).The acupuncture group showed significantly decreased TGF-β_1 m RNA transcription levels compared to the model group(P<0.01) while the bound group and model group showed no significant difference(P>0.05). The normal group displayed lower TGF-β_1 expression compared to the other 3 groups(P<0.01). Acupuncture group rats showed significantly decreased TGF-β_1 expression levels in the hippocampus and hypothalamus compared to model group rats(P<0.01); the bound group and model group showed no significant difference(P>0.05).Conclusion: Electric acupuncture can reduce TGF-β_1 m RNA transcription levels and TGF-β_1 protein expression in the hippocampus and hypothalamus of CFS model rats.
引文
[1]李立华,仇军,刘声,等.中医体质偏颇与慢性疲劳综合征的关系研究[J].世界中医药,2017,12:1171-1174,1178.
[2]鲁光宝,李旗,刘海洲.慢性疲劳综合征病因病机中西医方面的研究进展[J].中国当代医药,2009,16:12-13.
[3]陈任,方姝晨,宁宇,等.Ghrelin对小鼠认知功能的影响及机制探讨[J].神经损伤与功能重建,2015,10:471-473.
[4]吴雪艳.应激反应与抑郁症中下丘脑的性别差异:针对人脑样本和应激动物的研究[D].浙江大学,2016.
[5]余群星.基于Morris水迷宫学习记忆方法的研究[D].哈尔滨商业大学,2015.
[6]华金双,傅文,邵伯雍.逆针灸对慢性疲劳大鼠血清ACTH、CORT、CRH的影响[J].中医研究,2017,30:67-70.
[7]刘丰怡.俞募配穴法埋线治疗肝郁脾虚型慢性疲劳综合征的疗效观察[D].广州中医药大学,2014.
[8]杨启昭.背俞穴埋线干预慢性疲劳综合征大鼠的作用机制研究[D].广州中医药大学,2015.
[9]郑达理.借助立体定位术和荧光界定GFP裸小鼠脑解剖结构[D].苏州大学,2012.
[10]李安安.用于绘制高分辨小鼠全脑图谱的断层成像系统研究[D].华中科技大学,2010.
[11]邹显巍,刘爱东,余建萍.自发性癫痫大鼠皮质和海马中GLT-1、EAAC-1表达[J].神经损伤与功能重建,2016,11:293-295.
[12]秦小云,李桂成,张庆金,等.大鼠侧脑室注射Nesfatin-1对糖脂代谢的影响及其与下丘脑AMPK表达的关系[J].中风与神经疾病杂志,2017,34:901-904.
[13]姜凡.针刺治疗慢性疲劳综合征随机对照试验的系统评价[D].黑龙江中医药大学,2017.
[14]Jason LA,Katz BZ,Shiraishi Y,et al.Predictors of Post-Infectious Chronic Fatigue Syndrome in Adolescents[J].Health Psychol Behav Med,2014,2:41-51.
[15]Collin SM,Heron J,Nikolaus S,et al.Chronic fatigue syndrome(CFS/ME)symptom-based phenotypes and 1-year treatment outcomes in two clinical cohorts of adult patients in the UK and The Netherlands[J].JPsychosom Res,2018,104:29-34.
[16]Collin SM,Crawley E.Specialist treatment of chronic fatigue syndrome/ME:a cohort study among adult patients in England[J].BMCHealth Serv Res,2017,17:488.
[17]Clark LV,Pesola F,Thomas JM,et al.Guided graded exercise self-help plus specialist medical care versus specialist medical care alone for chronic fatigue syndrome(GETSET):a pragmatic randomised controlled trial[J].Lancet,2017,390:363-373.
[18]谭惠娟.针灸治疗慢性疲劳综合征的文献研究[D].成都中医药大学,2016.
[19]李元鑫.音乐电针治疗慢性疲劳综合征60例临床观察[J].黑龙江医药,2014,27:405-406.
[20]胡艺超.电针结合逍遥丸治疗慢性疲劳综合征的临床研究[D].广州中医药大学,2013.
[21]Ng SM,Yiu YM.Acupuncture for chronic fatigue syndrome:a randomized,sham-controlled trial with single-blinded desgin[J].Altern Ther Health Med,2013,19:21-26.
[22]E Rota,G Bellone,P Rocca,et al.Increased intrathecal TGF-beta1,but not IL-12,IFN-gamma and IL-10 levels in Alzheimer's disease patients[J].Neurol Sci,2006,27:33-39.
[23]Buckwalter MS,Yamane M,Coleman BS,et al.Chronically increased transforming growth factor-beta1 strongly inhibits hippocampal neurogenesis in aged mice[J].Am J Pathol,2006,169:154-164.
[24]Stipursky J,Gomes FC.TGF-β1/SMAD Signaling Induces Astrocyte Fate Commitment In Vitro:Implications for Radial Glia Development[J].Glia,2007,55:1023-1033.
[25]Vivien D,Ali C.Transforming growth factor-beta signalling in brain disorders[J].Cytokine Growth Factor Rev,2006,17:121-128.
[26]Wyller VB,Nguyen CB,Ludviksen JA,et al.Transforming growth factor beta(TGF-β)in adolescent chronic fatigue syndrome[J].J Transl Med,2017,15:245.
[27]朱兵.系统针灸学:复兴“体表医学”[M].北京:人民卫生出版社,2015:2-8.
[2]鲁光宝,李旗,刘海洲.慢性疲劳综合征病因病机中西医方面的研究进展[J].中国当代医药,2009,16:12-13.
[3]陈任,方姝晨,宁宇,等.Ghrelin对小鼠认知功能的影响及机制探讨[J].神经损伤与功能重建,2015,10:471-473.
[4]吴雪艳.应激反应与抑郁症中下丘脑的性别差异:针对人脑样本和应激动物的研究[D].浙江大学,2016.
[5]余群星.基于Morris水迷宫学习记忆方法的研究[D].哈尔滨商业大学,2015.
[6]华金双,傅文,邵伯雍.逆针灸对慢性疲劳大鼠血清ACTH、CORT、CRH的影响[J].中医研究,2017,30:67-70.
[7]刘丰怡.俞募配穴法埋线治疗肝郁脾虚型慢性疲劳综合征的疗效观察[D].广州中医药大学,2014.
[8]杨启昭.背俞穴埋线干预慢性疲劳综合征大鼠的作用机制研究[D].广州中医药大学,2015.
[9]郑达理.借助立体定位术和荧光界定GFP裸小鼠脑解剖结构[D].苏州大学,2012.
[10]李安安.用于绘制高分辨小鼠全脑图谱的断层成像系统研究[D].华中科技大学,2010.
[11]邹显巍,刘爱东,余建萍.自发性癫痫大鼠皮质和海马中GLT-1、EAAC-1表达[J].神经损伤与功能重建,2016,11:293-295.
[12]秦小云,李桂成,张庆金,等.大鼠侧脑室注射Nesfatin-1对糖脂代谢的影响及其与下丘脑AMPK表达的关系[J].中风与神经疾病杂志,2017,34:901-904.
[13]姜凡.针刺治疗慢性疲劳综合征随机对照试验的系统评价[D].黑龙江中医药大学,2017.
[14]Jason LA,Katz BZ,Shiraishi Y,et al.Predictors of Post-Infectious Chronic Fatigue Syndrome in Adolescents[J].Health Psychol Behav Med,2014,2:41-51.
[15]Collin SM,Heron J,Nikolaus S,et al.Chronic fatigue syndrome(CFS/ME)symptom-based phenotypes and 1-year treatment outcomes in two clinical cohorts of adult patients in the UK and The Netherlands[J].JPsychosom Res,2018,104:29-34.
[16]Collin SM,Crawley E.Specialist treatment of chronic fatigue syndrome/ME:a cohort study among adult patients in England[J].BMCHealth Serv Res,2017,17:488.
[17]Clark LV,Pesola F,Thomas JM,et al.Guided graded exercise self-help plus specialist medical care versus specialist medical care alone for chronic fatigue syndrome(GETSET):a pragmatic randomised controlled trial[J].Lancet,2017,390:363-373.
[18]谭惠娟.针灸治疗慢性疲劳综合征的文献研究[D].成都中医药大学,2016.
[19]李元鑫.音乐电针治疗慢性疲劳综合征60例临床观察[J].黑龙江医药,2014,27:405-406.
[20]胡艺超.电针结合逍遥丸治疗慢性疲劳综合征的临床研究[D].广州中医药大学,2013.
[21]Ng SM,Yiu YM.Acupuncture for chronic fatigue syndrome:a randomized,sham-controlled trial with single-blinded desgin[J].Altern Ther Health Med,2013,19:21-26.
[22]E Rota,G Bellone,P Rocca,et al.Increased intrathecal TGF-beta1,but not IL-12,IFN-gamma and IL-10 levels in Alzheimer's disease patients[J].Neurol Sci,2006,27:33-39.
[23]Buckwalter MS,Yamane M,Coleman BS,et al.Chronically increased transforming growth factor-beta1 strongly inhibits hippocampal neurogenesis in aged mice[J].Am J Pathol,2006,169:154-164.
[24]Stipursky J,Gomes FC.TGF-β1/SMAD Signaling Induces Astrocyte Fate Commitment In Vitro:Implications for Radial Glia Development[J].Glia,2007,55:1023-1033.
[25]Vivien D,Ali C.Transforming growth factor-beta signalling in brain disorders[J].Cytokine Growth Factor Rev,2006,17:121-128.
[26]Wyller VB,Nguyen CB,Ludviksen JA,et al.Transforming growth factor beta(TGF-β)in adolescent chronic fatigue syndrome[J].J Transl Med,2017,15:245.
[27]朱兵.系统针灸学:复兴“体表医学”[M].北京:人民卫生出版社,2015:2-8.