浙鲜9号的航天选育与特征特性分析
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摘要
为探究航天诱变对大豆主要农艺性状的影响,本研究比较了航天诱变选育新品种浙鲜9号与其亲本台湾75在生育期、产量、品质、抗病性等方面的差异。结果表明,浙鲜9号播种至采收生育期比亲本短2 d,株高矮7 cm,鲜百荚重高6. 1 g,鲜百粒重低2. 2 g,青荚色比亲本淡,两年区域试验平均鲜荚产量较亲本显著增加9. 4%。浙鲜9号不仅保留了亲本口感甜糯的优良品质,而且对大豆花叶病毒病的抗性有大幅度提高。利用60对核心SSR引物对二者进行分析,在Satt184、Satt197、Sat-213等10个标记间发现多态性位点,引物多态性率为16. 7%,Sat-213为大豆花叶病毒病抗性基因Rsc15相关分子标记,这从分子水平证实了浙鲜9号抗性的改良。采用100个SNP标记对二者进行分析,有5个SNP标记在二者之间存在差异。浙鲜9号与亲本主要特征特性和DNA分子标记的对比研究均充分证明了航天诱变育种的有效性和可靠性。
To clarify the effects of space mutagenesis on soybean agronomic characters,variations in maturity,yield,quality,and disease resistance were compared between space mutagenesis variety Zhexian No. 9 and its parent line Taiwan 75. The results showed that,Zhexian No.9 was two days earlier in maturity,7 cm lower in plant height,6. 1 g heavier in fresh 100-pod weight,2. 2 g lighter in fresh 100-seed weight,lighter in fresh pod color. The yield of Zhexian No.9 was significantly increased by 9. 4% compared with Taiwan 75. Zhexian No.9 not only retained the excellent sweet-taste quality of its parent line,but also the ability in SMV resistance was greatly improved. When comparing these two varieties using 60 pairs of SSR markers,10 markers including Satt184,Satt197 and Sat-213 were polymorphic between Zhexian No.9 and Taiwan 75,with a polymorphic rate of 16. 7%. Among them,Sat-213 was labeled as molecular marker for RSC15 resistance gene,confirming the improvements of disease resistance of Zhexian No.9 from molecular level. When analyzed with 100 SNP markers,5 markers were found to have polymorphism. The comparative study on the agronomic characteristics and the polymorphism of molecular markers between Zhexian No. 9 and its parent line gave us sufficient proof of the validity and reliability of space mutation breeding.
To clarify the effects of space mutagenesis on soybean agronomic characters,variations in maturity,yield,quality,and disease resistance were compared between space mutagenesis variety Zhexian No. 9 and its parent line Taiwan 75. The results showed that,Zhexian No.9 was two days earlier in maturity,7 cm lower in plant height,6. 1 g heavier in fresh 100-pod weight,2. 2 g lighter in fresh 100-seed weight,lighter in fresh pod color. The yield of Zhexian No.9 was significantly increased by 9. 4% compared with Taiwan 75. Zhexian No.9 not only retained the excellent sweet-taste quality of its parent line,but also the ability in SMV resistance was greatly improved. When comparing these two varieties using 60 pairs of SSR markers,10 markers including Satt184,Satt197 and Sat-213 were polymorphic between Zhexian No.9 and Taiwan 75,with a polymorphic rate of 16. 7%. Among them,Sat-213 was labeled as molecular marker for RSC15 resistance gene,confirming the improvements of disease resistance of Zhexian No.9 from molecular level. When analyzed with 100 SNP markers,5 markers were found to have polymorphism. The comparative study on the agronomic characteristics and the polymorphism of molecular markers between Zhexian No. 9 and its parent line gave us sufficient proof of the validity and reliability of space mutation breeding.
引文
[1]甘仪梅,张树珍,武媛丽,杨本鹏.作物航天诱变育种变异特征研究进展[J].广东农业科学,2015(1):119-123
[2]黄永相,郭涛,蔡金洋,刘永柱,张建国,王慧,陈志强.空间环境和60Co-γ辐照对水稻稻米品质的诱变效应[J].核农学报,2013,27(6):709-714
[3]郑伟,郭泰,王志新,李灿东,张振宇,郭美玲,王庆胜,张茂明,刘忠堂.大豆航天育种研究进展[J].辐射研究与辐射工艺学报,2015,33(5):1-9
[4]刘录祥,郭会君,赵林姝,古佳玉,赵世荣.我国作物航天育种20年的基本成就与展望[J].核农学报,2007,21(6):589-592
[5] Maluszynski M. Officially released mutant varieties the FAO/IAETdatabase[J]. Plant Cell Tissue and Organ Culture,2011,65(3):175-177
[6]俞法明,严文潮,毛雪琴,金庆生,李小华,陈蕾,陆艳婷.利用空间诱变技术进行早籼稻新品种的改良[J].核农学报,2014,28(6):949-954
[7]严文潮,孙国昌,俞法明,吴伟,徐建龙,刘庆龙,金庆生.早籼稻空间诱变新品种“浙101”的选育[J].核农学报,2006,20(5):398-400
[8] Gu R Q,Shen H M. Effects of space flight on the growth and somecytological characteristics of wheat seedlings[J]. ActaHotophysiologica Sinica,1989,15(4):403-407
[9]张宏纪,王广金,孙岩,刁艳玲,黄景华,郭强,刘东军,孙光祖.春小麦航天诱变入选后代的变异研究[J].核农学报,2007,21(2):111-115
[10] Mei M,Qin Y,Sun Y,Huang R,Yao J,Zhang Q,Hong M,Ye J.Morphological and molecular changes of maize plants after seeds beenflown on recoverable satellite[J]. Advances in Space Research,1998,22:1691-1697
[11]刘录祥,韩微波,郭会君,赵林姝,赵世荣,李家才,王晶,郑企成.高能混合粒子场诱变小麦的细胞学效应研究[J].核农学报,2005,19(5):327-331
[12] Nechitailo G S,Lu J Y,Xue H,Pan Y,Tang C Q,Liu M. Influence oflong term exposure to space flight on tomato seeds[J]. Adacances inSpace Research,2005,36(7):1329-1333
[13]郑积荣,汪炳良,王世恒,赵航萍,王慧俐.飞船搭载番茄种子SP1的生物学效应[J].核农学报,2004,18(4):311-313
[14]王世恒,祝水金,张雅,王艳芳.航天搭载茄子种子对其SP1生物学特性和SOD活性的影响[J].核农学报,2004,18(4):307-310
[15]李水凤,汪柄良,管学玉,张艳.卫星搭载处理对辣椒萌发过程中氧化酶活性的影响[J].核农学报,2006,20(4):432-436
[16]任卫波,韩建国,张蕴薇,郭慧琴,王致平.航天育种研究进展及其在草上的应用[J].中国草地学报,2006,28(5):91-97
[17]陈河龙,易克贤,马子龙,高建明,习金根,张世清,郑金龙,刘巧莲.柱花草航天育种研究进展[J].江西农业学报,2013,25(5):54-56
[18]吕爽,郑伟,郭泰,王志新,吴秀红,李灿东,张振宇,刘忠堂,张茂东,刘玉红,韩世峰,徐明贵.大豆航天搭载与60Co-γ辐射复合诱变效果分析[J].中国种业,2012(2):34-35
[19]赵曦,王广金,李铁,刘丽艳,谭巍巍,陆杰,付亚书,刘昭军.大豆空间诱变突变体的RAPD分析[J].核农学报,2014,28(5):772-776
[20]于绍轩,韩粉霞,孙君明,韩广振,葛一楠,闫淑荣,杨华.空间环境对大豆主要农艺性状及蛋白品质的诱变效应[J].核农学报,2010,24(3):453-459
[21]刘鑫磊,马岩松,栾晓燕,满为群,许德春,孟丽芬,付立新,赵晓南,刘琦.航天诱变对大豆品种光合性状影响[J].大豆科学,2011,30(4):606-608
[22]郑伟,郭泰,王志新.航天搭载大豆SP2代诱变效应初报[J].核农学报,2008,22(5):563-565
[23]郑伟,郭泰,王志新,吴秀红,刘忠堂,刘玉红,宋若鹤,陈永章.不同熟期大豆对航天诱变的反应[J].中国种业,2008(7):41-42
[24]张勇,杨兴勇,董全中,薛红,张明明,李微微,宋继玲,许德春,孟丽芬,付立新,赵晓楠,胡少新,刘录祥.利用空间诱变技术选育大豆新品种克山1号[J].核农学报,2013,27(9):1241-1246
[25]贾鸿昌,韩德志,闫洪睿,张雷,鹿文成,梁吉利,朱海芳.利用航天育种技术选育大豆新品种金源55号[J].核农学报,2015,29(6):1025-2029
[26]郭泰,刘忠堂,吕秀珍,王志新,吴秀红,郑伟.合丰号的辐射诱变育种回顾[J].核农学报,2010,24(2):292-297
[27] Permingeat H R,Romagnoli MⅤ,Sesma JⅠ,Vallejos R H(1998)A simple method for isolating DNA of high yield and qualityfrom cotton(Gossypium hirsutum L.)leaves[J]. Plant MolecularBiology Reqevter,1998,16(1):89
[28]中国疾病预防控制中心营养与食品安全所. GB/T 5009. 8-2008食品中蔗糖的测定[S].北京:中国标准出版社,2008
[29]中疾病预防控制中心营养与食品安全所. GB/T 5009. 9-2008食品中淀粉的测定[S].北京:中国标准出版社,2008
[30] Rui R,Liu S C,Karthikeyan A,Wang T,Niu H P,Yin J L,YangY H,Wang L Q,Yang Q H,Zhi H J,Li K. Fine-mapping andidentification of a novel locus Rsc15 underlying soybean resistance toSoybean mosaic virus[J]. Theoretical and Applied Genetics,2017,130(11):2395-2410
[31]张宏纪,刁艳玲,孙连发,孙岩,刘东军,郭强,兰静,黄景华,杨淑萍,孙光祖.航天诱变新品种龙福麦18的选育及其主要特征特性分析[J].核农学报,2008,22(3):243-247
[32]方金梁,邹定斌,周永胜,宁娇丽.航天诱变选育高产高蛋白质水稻新品种[J].核农学报,2004,18(4):280-283
[33]杜久元,白斌,金明安,何春雨,包文生,张廷刚,周祥椿.小麦航天诱变后代条锈病抗性变异及选择效果的研究[J].麦类作物学报,2012,32(4):767-773
[34]蒋凌雪,刘章雄,任洪雷,金龙国,张洪岩,郭勇,陶波,邱丽娟.空间诱变后大豆对草甘膦的耐性研究[J].核农学报,2011,25(5):844-850
[2]黄永相,郭涛,蔡金洋,刘永柱,张建国,王慧,陈志强.空间环境和60Co-γ辐照对水稻稻米品质的诱变效应[J].核农学报,2013,27(6):709-714
[3]郑伟,郭泰,王志新,李灿东,张振宇,郭美玲,王庆胜,张茂明,刘忠堂.大豆航天育种研究进展[J].辐射研究与辐射工艺学报,2015,33(5):1-9
[4]刘录祥,郭会君,赵林姝,古佳玉,赵世荣.我国作物航天育种20年的基本成就与展望[J].核农学报,2007,21(6):589-592
[5] Maluszynski M. Officially released mutant varieties the FAO/IAETdatabase[J]. Plant Cell Tissue and Organ Culture,2011,65(3):175-177
[6]俞法明,严文潮,毛雪琴,金庆生,李小华,陈蕾,陆艳婷.利用空间诱变技术进行早籼稻新品种的改良[J].核农学报,2014,28(6):949-954
[7]严文潮,孙国昌,俞法明,吴伟,徐建龙,刘庆龙,金庆生.早籼稻空间诱变新品种“浙101”的选育[J].核农学报,2006,20(5):398-400
[8] Gu R Q,Shen H M. Effects of space flight on the growth and somecytological characteristics of wheat seedlings[J]. ActaHotophysiologica Sinica,1989,15(4):403-407
[9]张宏纪,王广金,孙岩,刁艳玲,黄景华,郭强,刘东军,孙光祖.春小麦航天诱变入选后代的变异研究[J].核农学报,2007,21(2):111-115
[10] Mei M,Qin Y,Sun Y,Huang R,Yao J,Zhang Q,Hong M,Ye J.Morphological and molecular changes of maize plants after seeds beenflown on recoverable satellite[J]. Advances in Space Research,1998,22:1691-1697
[11]刘录祥,韩微波,郭会君,赵林姝,赵世荣,李家才,王晶,郑企成.高能混合粒子场诱变小麦的细胞学效应研究[J].核农学报,2005,19(5):327-331
[12] Nechitailo G S,Lu J Y,Xue H,Pan Y,Tang C Q,Liu M. Influence oflong term exposure to space flight on tomato seeds[J]. Adacances inSpace Research,2005,36(7):1329-1333
[13]郑积荣,汪炳良,王世恒,赵航萍,王慧俐.飞船搭载番茄种子SP1的生物学效应[J].核农学报,2004,18(4):311-313
[14]王世恒,祝水金,张雅,王艳芳.航天搭载茄子种子对其SP1生物学特性和SOD活性的影响[J].核农学报,2004,18(4):307-310
[15]李水凤,汪柄良,管学玉,张艳.卫星搭载处理对辣椒萌发过程中氧化酶活性的影响[J].核农学报,2006,20(4):432-436
[16]任卫波,韩建国,张蕴薇,郭慧琴,王致平.航天育种研究进展及其在草上的应用[J].中国草地学报,2006,28(5):91-97
[17]陈河龙,易克贤,马子龙,高建明,习金根,张世清,郑金龙,刘巧莲.柱花草航天育种研究进展[J].江西农业学报,2013,25(5):54-56
[18]吕爽,郑伟,郭泰,王志新,吴秀红,李灿东,张振宇,刘忠堂,张茂东,刘玉红,韩世峰,徐明贵.大豆航天搭载与60Co-γ辐射复合诱变效果分析[J].中国种业,2012(2):34-35
[19]赵曦,王广金,李铁,刘丽艳,谭巍巍,陆杰,付亚书,刘昭军.大豆空间诱变突变体的RAPD分析[J].核农学报,2014,28(5):772-776
[20]于绍轩,韩粉霞,孙君明,韩广振,葛一楠,闫淑荣,杨华.空间环境对大豆主要农艺性状及蛋白品质的诱变效应[J].核农学报,2010,24(3):453-459
[21]刘鑫磊,马岩松,栾晓燕,满为群,许德春,孟丽芬,付立新,赵晓南,刘琦.航天诱变对大豆品种光合性状影响[J].大豆科学,2011,30(4):606-608
[22]郑伟,郭泰,王志新.航天搭载大豆SP2代诱变效应初报[J].核农学报,2008,22(5):563-565
[23]郑伟,郭泰,王志新,吴秀红,刘忠堂,刘玉红,宋若鹤,陈永章.不同熟期大豆对航天诱变的反应[J].中国种业,2008(7):41-42
[24]张勇,杨兴勇,董全中,薛红,张明明,李微微,宋继玲,许德春,孟丽芬,付立新,赵晓楠,胡少新,刘录祥.利用空间诱变技术选育大豆新品种克山1号[J].核农学报,2013,27(9):1241-1246
[25]贾鸿昌,韩德志,闫洪睿,张雷,鹿文成,梁吉利,朱海芳.利用航天育种技术选育大豆新品种金源55号[J].核农学报,2015,29(6):1025-2029
[26]郭泰,刘忠堂,吕秀珍,王志新,吴秀红,郑伟.合丰号的辐射诱变育种回顾[J].核农学报,2010,24(2):292-297
[27] Permingeat H R,Romagnoli MⅤ,Sesma JⅠ,Vallejos R H(1998)A simple method for isolating DNA of high yield and qualityfrom cotton(Gossypium hirsutum L.)leaves[J]. Plant MolecularBiology Reqevter,1998,16(1):89
[28]中国疾病预防控制中心营养与食品安全所. GB/T 5009. 8-2008食品中蔗糖的测定[S].北京:中国标准出版社,2008
[29]中疾病预防控制中心营养与食品安全所. GB/T 5009. 9-2008食品中淀粉的测定[S].北京:中国标准出版社,2008
[30] Rui R,Liu S C,Karthikeyan A,Wang T,Niu H P,Yin J L,YangY H,Wang L Q,Yang Q H,Zhi H J,Li K. Fine-mapping andidentification of a novel locus Rsc15 underlying soybean resistance toSoybean mosaic virus[J]. Theoretical and Applied Genetics,2017,130(11):2395-2410
[31]张宏纪,刁艳玲,孙连发,孙岩,刘东军,郭强,兰静,黄景华,杨淑萍,孙光祖.航天诱变新品种龙福麦18的选育及其主要特征特性分析[J].核农学报,2008,22(3):243-247
[32]方金梁,邹定斌,周永胜,宁娇丽.航天诱变选育高产高蛋白质水稻新品种[J].核农学报,2004,18(4):280-283
[33]杜久元,白斌,金明安,何春雨,包文生,张廷刚,周祥椿.小麦航天诱变后代条锈病抗性变异及选择效果的研究[J].麦类作物学报,2012,32(4):767-773
[34]蒋凌雪,刘章雄,任洪雷,金龙国,张洪岩,郭勇,陶波,邱丽娟.空间诱变后大豆对草甘膦的耐性研究[J].核农学报,2011,25(5):844-850