外源肠道菌和玉米抗性淀粉对小鼠结肠菌群发酵产酸的影响
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摘要
在37℃、厌氧条件下,通过向营养肉汤培养基中添加小鼠结肠内容物悬液模拟结肠环境。通过在模拟结肠环境中添加玉米抗性淀粉、单一及混合外源肠道菌及其组合,研究玉米抗性淀粉体外发酵产酸的特性及外源肠道菌对其的影响。结果表明:相对于未添加抗性淀粉或仅添加单一外源菌的组,添加3%(相对于营养肉汤培养基干基)玉米抗性淀粉后,乙酸、丙酸和丁酸的含量在发酵过程中(6、12、18、24、30 h)能够极显著(P<0.01)地提升。在含有抗性淀粉的培养基中添加单一外源菌,嗜酸乳杆菌能够促进发酵产乙酸,青春双歧杆菌和粪肠球菌能够促进发酵产丙酸和丁酸,而大肠埃希氏菌会抑制产丙酸和丁酸。在含有抗性淀粉的培养基中添加混合外源菌,嗜酸乳杆菌和青春双歧杆菌能够协同发酵产乙酸、丙酸和丁酸,粪肠球菌与青春双歧杆菌组合发酵时,能够提升丙酸和丁酸产量,而大肠埃希氏菌则会不同程度地抑制其他3种菌发酵抗性淀粉产乙酸、丙酸和丁酸。
Simulated colonic fermentation was conducted in nutrient broth with colon content suspension from mice at 37 ℃ under anaerobic condition. The fermentation characteristics of corn resistant starch and the influence of exogenous intestinal bacteria on these characteristics were studied by adding corn resistant starch and single or mixed exogenous intestinal bacteria or their compound to the simulated colonic environment and measuring the short chain fatty acid concentration. The results showed that the concentration of acetic acid,propionic acid and butyric acid was significantly increased( P < 0. 01) during fermentation at 6,12,18,24 and 30 h with the addition of 3% corn resistant starch( compared with the dry nutrient broth medium),compared with the control group or the group with single exogenous intestinal bacteria. Four kinds of exogenous bacteria were added to the fermentation broth containing corn resistant starch separately. The result showed that the production of acetic acid was promoted with the addition of lactobacillus acidophilus and the production of propionic acid and butyric acid was promoted by bifidobacterium adolescentis and enterococcus faecalis,while the production of propionic acid and butyric acid was inhibited by escherichia coli. Three pairs of exogenous bacteria cocktail were added to the fermentation broth containing corn resistant starch. It was found that the combination of bifidobacterium adolescentis and lactobacillus acidophilus could synergistically promote the production of acetic acid,propionic acid and butyric acid during fermentation,and the combination of bifidobacterium adolescentis and enterococcus faecalis could also promote the production of propionic acid and butyric acid,but escherichia coli Would inhibit the other three kinds of bacteria producing short chain fatty acid to different degrees during fermentation.
Simulated colonic fermentation was conducted in nutrient broth with colon content suspension from mice at 37 ℃ under anaerobic condition. The fermentation characteristics of corn resistant starch and the influence of exogenous intestinal bacteria on these characteristics were studied by adding corn resistant starch and single or mixed exogenous intestinal bacteria or their compound to the simulated colonic environment and measuring the short chain fatty acid concentration. The results showed that the concentration of acetic acid,propionic acid and butyric acid was significantly increased( P < 0. 01) during fermentation at 6,12,18,24 and 30 h with the addition of 3% corn resistant starch( compared with the dry nutrient broth medium),compared with the control group or the group with single exogenous intestinal bacteria. Four kinds of exogenous bacteria were added to the fermentation broth containing corn resistant starch separately. The result showed that the production of acetic acid was promoted with the addition of lactobacillus acidophilus and the production of propionic acid and butyric acid was promoted by bifidobacterium adolescentis and enterococcus faecalis,while the production of propionic acid and butyric acid was inhibited by escherichia coli. Three pairs of exogenous bacteria cocktail were added to the fermentation broth containing corn resistant starch. It was found that the combination of bifidobacterium adolescentis and lactobacillus acidophilus could synergistically promote the production of acetic acid,propionic acid and butyric acid during fermentation,and the combination of bifidobacterium adolescentis and enterococcus faecalis could also promote the production of propionic acid and butyric acid,but escherichia coli Would inhibit the other three kinds of bacteria producing short chain fatty acid to different degrees during fermentation.
引文
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[19]汪婕,谢涛.锥栗抗性淀粉消化前后的益生作用及结构变化研究[J].食品工业科技,2014,35(13):61-65WANG J,XIE T.Probiotic functions and structural changes of castanea henryi resistant starch before and after digestion[J].Science and Technology of Food Industry,2014,35(13):61-65
[20]HAENEN D,SOUZAD S C,ZHANG J,et al.Resistant starch induces catabolic but suppresses immune and cell division pathways and changes the microbiome in the proximal colon of male pigs[J].Journal of Nutrition,2013,143(12):1889-1898.
[2]FUENTES-ZARAGOZA E,RIQUELME-NAVARRETE M J,SáNCHEZ-ZAPATA E,et al.Resistant starch as functional ingredient:a review[J].Food Research International,2010,43(4):931-942
[3]朱平,孔祥礼,包劲松.抗性淀粉在食品中的应用及功效研究进展[J].核农学报,2015,29(2):327-336ZHU P,KONG X L,BAO J S.Current progress on the applications and health benefits of resistant starch in foods[J].Acta Agriculturae Nucleatae Sinica,2015,2:327-336
[4]REDDY C K,HARIPRIYA S,MOHAMED A N,et al.Preparation and characterization of resistant starch III from elephant foot yam(Amorphophallus paeonifolius)starch[J].Food Chemistry,2014,155(5):38-44
[5]SANKHON A,YAO W R,WANG H Y,et al.The yield of improvement of resistant starches from Africa Locust(Parkia biglobosa):the influence of heat-moisture,autoclavingcooling and cross-linking treatments[J].American Journal of Food Technology,2012,7(7):386-397
[6]TAN Z N,ROSMA A,NAPISAH H,et al.Characteristics of metroxylon sagu resistant starch type III as prebiotic substance[J].Journal of Food Science,2015,80(4):H875-H882
[7]CHATELIER E L,NIELSEN T,QIN J,et al.Richness of human gut microbiome correlates with metabolic markers[J].Nature,2013,500(7464):541-546
[8]MAHADEVAMMA S,SHAMALA T R,THARANATHAN R N.Resistant starch derived from processed legumes:in vitro and in vivo fermentation characteristics[J].International Journal of Food Science and Nutrition,2004,55(5):399-405
[9]VINCENT Lebet,EVA Arrigoni,RENATO Amadò.Measurement of fermentation products and substrate disappearance during incubation of dietary fibre sources with human faecal flora[J].Academic Press,1998,31(5):473-479
[10]谢涛,张淑远,王美桂,等.重结晶红薯淀粉体外消化前后益生作用与结构变化[J].农业机械学报,2013,44(8):202-206XIE T,ZHANG S Y,WANG M G,et al.Probiotic functions and structure changes of recrystallized sweet potato starch before and after in vitro digestion[J].Transactions of the Chinese Society of Agricultural Machinery,2013,44(8):202-206
[11]ZHANG H Z,XU X M,JIN Z Y.Fermentation characteristics of resistant starch from maize prepared by the enzymatic method in vitro[J].International Journal of Biological Macromolecules,2012,51(5):1185-8
[12]HEDEMANN M S,KNUDSEN K E B.Resistant starch for weaning pigs:Effect on concentration of short chain fatty acids in digestion and intestinal morphology[J].Livestock Science,2007,108(1):175-177
[13]庞秋芳,彭喜春,欧仕益,等.直肠菌群对两种抗性淀粉的体外发酵研究[J].食品工业科技,2011(4):148-151PANG Q F,PENG X C,OU S Y,et al.Study on fermentation of two resistant starch by colon microflora in vitro[J].Science and Technology of Food Industry,2011(04):148-151
[14]王志凡,杨秀琳,陈旺盛,等.抗性淀粉对饮食诱导肥胖大鼠排便状况及肠道菌群的影响[J].动物营养学报,2016,28(5):1626-1632WANG Z F,YANG X L,CHEN W S,et al.Effects of resistant starch on defecation and intestinal microflora of diet induced obesity rats[J].Chinese Journal of Animal Nutrition,2016,28(5):1626-1632
[15]谢涛,曾红华,汪婕,等.4种抗性淀粉的益生作用及结构变化[J].中国粮油学报,2014,29(10):23-27XIE T,ZENG H H,WANG J,et al.Probiotic functions and structural changes of 4 resistant starches[J].Journal of the Chinese Cereals and Oils Association,2014,29(10):23-27
[16]彭真福,陈庆发,白永亮,等.双歧杆菌对香蕉抗性淀粉的体外发酵研究[J].食品科技,2015(1):2-5PENG Z F,CHEN Q F,BAI Y L,et al.Fermentation of banana resistant starch by bifidobacterium in vitro[J].Food Science and Technology,2015(1):2-5
[17]朱翠兰.玉米抗性淀粉的模拟肠道发酵产酸与外源乳酸菌关联性[D].哈尔滨:东北农业大学,2012ZHU C L.Acid production of crude maize resistant starch in the simulated fermentation in vitro and the Impacts of some exogenous lactic acid bacteria added[D].Haerbin:Northeast Agricultural University,2012
[18]连晓蔚.肠道菌群利用几种膳食纤维体外发酵产短链脂肪酸的研究[D].广州:暨南大学,2011LIAN X W.Gut microbiota in vitro fermenting destarched dietary fiber to produce SCFA[D].Guangzhou:Jinan University,2011
[19]汪婕,谢涛.锥栗抗性淀粉消化前后的益生作用及结构变化研究[J].食品工业科技,2014,35(13):61-65WANG J,XIE T.Probiotic functions and structural changes of castanea henryi resistant starch before and after digestion[J].Science and Technology of Food Industry,2014,35(13):61-65
[20]HAENEN D,SOUZAD S C,ZHANG J,et al.Resistant starch induces catabolic but suppresses immune and cell division pathways and changes the microbiome in the proximal colon of male pigs[J].Journal of Nutrition,2013,143(12):1889-1898.