牛奶中四环素类兽残的快速检测关键技术应用研究
|
摘要
介绍四环素类兽残快速检测的几种常用方法及区别,并就目前市场上3种典型牛奶用液相色谱-质谱/质谱法,进行土霉素、四环素、金霉素、强力霉素4种抗生素检测,结果表明3种牛奶中四环素含量均低于检出限,效果较好。
Several common rapid testing methods and their difference of veterinary drug residues were introduced in this paper. Three typical kinds of milk were detected by liquid chromatography-mass spectrum/mass spectrometry. The results of four kinds of antibiotics,which were oxytetracycline,tetracycline,chlortetracycline and doxycycline,were lower than the detection limits and were better.
Several common rapid testing methods and their difference of veterinary drug residues were introduced in this paper. Three typical kinds of milk were detected by liquid chromatography-mass spectrum/mass spectrometry. The results of four kinds of antibiotics,which were oxytetracycline,tetracycline,chlortetracycline and doxycycline,were lower than the detection limits and were better.
引文
[1]方晓明,丁卓平.动物源食品兽药残留分析[M].北京:化学工业出版社,2008
[2]顿文涛,李勉,毕庆生,等.用于抗生素检测的生物传感器研究进展[J].生物技术通报,2013(6):70-74
[3]Oka H,Ito Y,Matsumoto H.Chromatographic analysis of tetracycline antibiotics in foods[J].J.Chromatogr.A,2000,882:109-133
[4]Cháfer-Pericása C,Maquieira魣,Puchades R,et al.Immunochemical determination of oxytetracycline in fish:Comparison between enzymatic and time-resolved fluorometric[J].Anal Chim Acta,2010,662(2):177-185
[5]师邱毅,纪其雄,许莉勇.食品安全快速检测技术及应用[M].北京:化学工业出版社,2010
[6]钱琛,李静,陈桂良.动物源性食品兽药残留分析中样品前处理方法的研究进展[J].食品安全质量检测学报,2015(5):1666-1674
[7]王志强,胡国媛,李志勇.微生物抑制法快速检测动物源性食品多种抗生素残留[J].中国卫生检验杂志,2008,18(9):1732-1734
[8]黄晓蓉,郑晶,吴谦.食品中多种抗生素残留的微生物筛检方法研究[J].食品科学,2007,28(8):418-421
[9]付云洁,刘志国,武玉香.胶体金免疫层析法快速检测食品中金霉素残留[J].食品科学,2010,31(2):191-194
[10]刘智宏,叶妮,郭文林.四环素类药物多残留酶联免疫检测方法[J].中国农业科学,2009,42(1):318-323
[11]戚洁阳.乳制品中抗生素残留量高效液相色谱分析方法研究[D].上海:复旦大学,2011
[12]李琴,姜金斗,姜全忠,等,生物芯片技术用于同时测定乳与乳粉中β-兴奋剂类残留量[J].中国乳品工业,2014,42(2):52-54,64
[13]穆小婷,董文宾,王玲玲,等.特异性生物芯片在乳品检测中的应用[J].中国乳品工业,2014,42(2):38-40,43
[14]李延华,王伟军,张兰威,等.试管扩散法与国标TTC法检测牛乳中β-内酰胺类抗生素残留[J].食品科学,2008,29(6):252-254
[15]佚名.新型Delvotest~BLF-快速便捷,值得信赖[J].中国乳品工业,2012,40(4):61
[16]JEON M,KIM J,PAENG K J,et al.Biotin-avidin mediated competitive enzyme-linked immunosorbent assay to detect residues of tetracyclines in milk[J].Microchemical Journal,2007,88(1):26-31
[17]KNECHT B G,STRANSER A,DIETRICH R,et al.Automated Microarray System for the Simultaneous Detection of Antibiotics in Milk[J].Analytical Chemistry,2004,76(3):646-654
[18]ZHOU Y,LI C Y,LI Y S,et al.Monoclonal antibody based inhibition ELISA as a new tool for the analysis of melamine in milk and pet food samples[J].Food Chemistry,2012,135(4):2681-2686
[19]GAUDIN V,CADIEU N,SANDERS P.Results of a European proficiency test for the detection of streptomycin/dihydrostreptomycin,gentamicin and neomycin in milk by ELISA and biosensor methods[J].Analytica Chimica Acta,2004,529(1):273-283
[20]Zhang J,Zhang B,Wu Y,et al.Fast determination of the tetracyclines in milk samples by the aptamer biosensor[J].Analyst,2010,135(10):2706-2710
[21]吴艳,张娟琨,范婷,等.核酸适体生物传感器快速检测牛奶中抗生素[J].生物加工过程,2010,8(3):48-52
[22]Fernández F,HegnerováK,Piliarik M,et al.A label-free and portable multichannel surface plasmon resonance immunosensor for onsite analysis of antibiotics in milk samples[J].Biosens Bioelectron,2010,26(4):1231-1238
[23]Adrian J,Pasche S,Diserens JM,et al.Waveguide interrogated optical immunosensor(WIOS)for detection of sulfonamide antibiotics in milk[J].Biosens Bioelectron,2009,24(11):3340-3346
[24]NAOTO T,MASASHI K,HARUMA K,et al.A Quartz Crystal Microbalance Sensor Coated with MIP for“Bisphenol A”and Its Proper-ties[J].Thin Solid Films,2006,499:380-385
[25]Suárez G,Jin YH,Auerswald J,et al.Lab-on-a-chip for multiplexed biosensing of residual antibiotics in milk[J].Lab Chip,2009,9(11):1625-1630
[26]张利萍,吕运开,王晓虎.基于限进介质-磁性微球的磁分散萃取-液相色谱法同时检测牛奶中四环素类药物残留[J].食品安全质量检测学报,2014,5(2):377-383
[27]邓江华.醇与小分子有机溶剂二元双水相分离萃取四环素类抗生素[D].西安:长安大学,2013
[28]Wierucka M,Biziuk M.Application of magnetic nanoparticles for magnetic solid-phase extraction in preparing biological,environmental and food samples[J].Trends Anal Chem,2014,59:50-58
[29]王丽敏.分子印迹杂化复合材料的合成及选择性固相萃取食品中四环素类兽药残留[D].保定:河北大学,2012
[30]彭凌雪.小分子醇/盐二元双水相体系分离/萃取四环素类抗生素的研究[D].西安:长安大学,2014
[31]Mu GF,Liu HT,Xu LN,et al.Matrix solid-phase dispersion extraction and capillary electrophoresis determination of tetracycline residues in milk[J].Food Anal Method,2012,5:148-153
[32]苏明明,王翛,王晓薇,等.放射免疫法快速检测牛血清中四环素类药物[J].食品安全质量检测学报,2016(2):629-633
[33]Pastor-Navarro N,Morais S,Maquieira A,et al.Synthesis of haptens and development of a sensitive immunoassay for tetracycline residues.Applicationto honey samples[J].Anal Chim Acta,2007,594(2):211-218
[34]刘津涛.四环素酶联免疫检测方法的研究[D].天津:天津科技大学,2013
[35]方莹.四环素免疫金快速检测技术研究[D].杭州:浙江大学,2010
[36]唐海波,齐维,唐时幸.荧光定量法快速检测鲜奶中四环素类总量残留[J].现代食品科技,2012(11):1600-1602
[37]叶永丽,高旭东,赫欣睿,等.SPE-HPLC法检测早胜牛肉中土霉素、四环素和金霉素的残留[J].国际药学研究杂志,2016(2):382-386
[38]Hird SJ,Lau BPY,Schuhmacher R,et al.Liquid chromatographymass spectrometry for the determination of chemical contaminants in food[J].Trends Anal Chem,2014,59:59-72
[39]郭杰,张朝飞,高庚渠,等.高效液相色谱-串联质谱联用在牛奶抗生素残留检测中的研究进展[J].中国畜牧兽医,2014(1):236-239
[40]魻nal A.Overview on liquid chromatographic analysis of tetracycline residues in food matrices[J].Food Chem,2011,127(1):197-203
[41]葛晓鸣,湛嘉,杨亮,等.动物源性食品中小分子有机污染物的多类别多残留分析技术的研究进展[J].理化检验(化学分册),2015(3):409-415
[42]许莉,李可强.乳品中可能含有的不安全物质及其快速检测方法[J].中国乳品工业,2016(1):38-43
[43]佚名.中国乳品检测系统将与欧盟同步[J].养殖与饲料,2014(4):41
[2]顿文涛,李勉,毕庆生,等.用于抗生素检测的生物传感器研究进展[J].生物技术通报,2013(6):70-74
[3]Oka H,Ito Y,Matsumoto H.Chromatographic analysis of tetracycline antibiotics in foods[J].J.Chromatogr.A,2000,882:109-133
[4]Cháfer-Pericása C,Maquieira魣,Puchades R,et al.Immunochemical determination of oxytetracycline in fish:Comparison between enzymatic and time-resolved fluorometric[J].Anal Chim Acta,2010,662(2):177-185
[5]师邱毅,纪其雄,许莉勇.食品安全快速检测技术及应用[M].北京:化学工业出版社,2010
[6]钱琛,李静,陈桂良.动物源性食品兽药残留分析中样品前处理方法的研究进展[J].食品安全质量检测学报,2015(5):1666-1674
[7]王志强,胡国媛,李志勇.微生物抑制法快速检测动物源性食品多种抗生素残留[J].中国卫生检验杂志,2008,18(9):1732-1734
[8]黄晓蓉,郑晶,吴谦.食品中多种抗生素残留的微生物筛检方法研究[J].食品科学,2007,28(8):418-421
[9]付云洁,刘志国,武玉香.胶体金免疫层析法快速检测食品中金霉素残留[J].食品科学,2010,31(2):191-194
[10]刘智宏,叶妮,郭文林.四环素类药物多残留酶联免疫检测方法[J].中国农业科学,2009,42(1):318-323
[11]戚洁阳.乳制品中抗生素残留量高效液相色谱分析方法研究[D].上海:复旦大学,2011
[12]李琴,姜金斗,姜全忠,等,生物芯片技术用于同时测定乳与乳粉中β-兴奋剂类残留量[J].中国乳品工业,2014,42(2):52-54,64
[13]穆小婷,董文宾,王玲玲,等.特异性生物芯片在乳品检测中的应用[J].中国乳品工业,2014,42(2):38-40,43
[14]李延华,王伟军,张兰威,等.试管扩散法与国标TTC法检测牛乳中β-内酰胺类抗生素残留[J].食品科学,2008,29(6):252-254
[15]佚名.新型Delvotest~BLF-快速便捷,值得信赖[J].中国乳品工业,2012,40(4):61
[16]JEON M,KIM J,PAENG K J,et al.Biotin-avidin mediated competitive enzyme-linked immunosorbent assay to detect residues of tetracyclines in milk[J].Microchemical Journal,2007,88(1):26-31
[17]KNECHT B G,STRANSER A,DIETRICH R,et al.Automated Microarray System for the Simultaneous Detection of Antibiotics in Milk[J].Analytical Chemistry,2004,76(3):646-654
[18]ZHOU Y,LI C Y,LI Y S,et al.Monoclonal antibody based inhibition ELISA as a new tool for the analysis of melamine in milk and pet food samples[J].Food Chemistry,2012,135(4):2681-2686
[19]GAUDIN V,CADIEU N,SANDERS P.Results of a European proficiency test for the detection of streptomycin/dihydrostreptomycin,gentamicin and neomycin in milk by ELISA and biosensor methods[J].Analytica Chimica Acta,2004,529(1):273-283
[20]Zhang J,Zhang B,Wu Y,et al.Fast determination of the tetracyclines in milk samples by the aptamer biosensor[J].Analyst,2010,135(10):2706-2710
[21]吴艳,张娟琨,范婷,等.核酸适体生物传感器快速检测牛奶中抗生素[J].生物加工过程,2010,8(3):48-52
[22]Fernández F,HegnerováK,Piliarik M,et al.A label-free and portable multichannel surface plasmon resonance immunosensor for onsite analysis of antibiotics in milk samples[J].Biosens Bioelectron,2010,26(4):1231-1238
[23]Adrian J,Pasche S,Diserens JM,et al.Waveguide interrogated optical immunosensor(WIOS)for detection of sulfonamide antibiotics in milk[J].Biosens Bioelectron,2009,24(11):3340-3346
[24]NAOTO T,MASASHI K,HARUMA K,et al.A Quartz Crystal Microbalance Sensor Coated with MIP for“Bisphenol A”and Its Proper-ties[J].Thin Solid Films,2006,499:380-385
[25]Suárez G,Jin YH,Auerswald J,et al.Lab-on-a-chip for multiplexed biosensing of residual antibiotics in milk[J].Lab Chip,2009,9(11):1625-1630
[26]张利萍,吕运开,王晓虎.基于限进介质-磁性微球的磁分散萃取-液相色谱法同时检测牛奶中四环素类药物残留[J].食品安全质量检测学报,2014,5(2):377-383
[27]邓江华.醇与小分子有机溶剂二元双水相分离萃取四环素类抗生素[D].西安:长安大学,2013
[28]Wierucka M,Biziuk M.Application of magnetic nanoparticles for magnetic solid-phase extraction in preparing biological,environmental and food samples[J].Trends Anal Chem,2014,59:50-58
[29]王丽敏.分子印迹杂化复合材料的合成及选择性固相萃取食品中四环素类兽药残留[D].保定:河北大学,2012
[30]彭凌雪.小分子醇/盐二元双水相体系分离/萃取四环素类抗生素的研究[D].西安:长安大学,2014
[31]Mu GF,Liu HT,Xu LN,et al.Matrix solid-phase dispersion extraction and capillary electrophoresis determination of tetracycline residues in milk[J].Food Anal Method,2012,5:148-153
[32]苏明明,王翛,王晓薇,等.放射免疫法快速检测牛血清中四环素类药物[J].食品安全质量检测学报,2016(2):629-633
[33]Pastor-Navarro N,Morais S,Maquieira A,et al.Synthesis of haptens and development of a sensitive immunoassay for tetracycline residues.Applicationto honey samples[J].Anal Chim Acta,2007,594(2):211-218
[34]刘津涛.四环素酶联免疫检测方法的研究[D].天津:天津科技大学,2013
[35]方莹.四环素免疫金快速检测技术研究[D].杭州:浙江大学,2010
[36]唐海波,齐维,唐时幸.荧光定量法快速检测鲜奶中四环素类总量残留[J].现代食品科技,2012(11):1600-1602
[37]叶永丽,高旭东,赫欣睿,等.SPE-HPLC法检测早胜牛肉中土霉素、四环素和金霉素的残留[J].国际药学研究杂志,2016(2):382-386
[38]Hird SJ,Lau BPY,Schuhmacher R,et al.Liquid chromatographymass spectrometry for the determination of chemical contaminants in food[J].Trends Anal Chem,2014,59:59-72
[39]郭杰,张朝飞,高庚渠,等.高效液相色谱-串联质谱联用在牛奶抗生素残留检测中的研究进展[J].中国畜牧兽医,2014(1):236-239
[40]魻nal A.Overview on liquid chromatographic analysis of tetracycline residues in food matrices[J].Food Chem,2011,127(1):197-203
[41]葛晓鸣,湛嘉,杨亮,等.动物源性食品中小分子有机污染物的多类别多残留分析技术的研究进展[J].理化检验(化学分册),2015(3):409-415
[42]许莉,李可强.乳品中可能含有的不安全物质及其快速检测方法[J].中国乳品工业,2016(1):38-43
[43]佚名.中国乳品检测系统将与欧盟同步[J].养殖与饲料,2014(4):41