灵芝养肝丸的质量标准研究
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摘要
目的:建立灵芝养肝丸质量标准。方法:采用显微鉴别法对猪苓、大黄、赤芍、黄连、厚朴、甘草进行鉴别,用薄层色谱法对大黄、黄连进行鉴别;用高效液相色谱法测定赤芍中芍药苷的含量。结果:薄层色谱斑点清晰;芍药苷在0.06～1.8μg(r=0.9999)的范围内线性关系良好,平均回收率为98.41%(n=6),RSD为0.63%。方法精密度,重复性符合要求。结论:该方法结果准确可靠,适用于灵芝养肝丸的质量控制。
Objective To establish the quality standard of Ganoderma lucidum liver pill. Methods Lingling, rhubarb, radix paeoniae, Huanglian, Magnolia and licorice were identified by microscopic identification, and rhubarb and Huanglian were identified by TLC, and the content of Paeoniflorin in Radix paeoniae was determined by high performance liquid chromatography.Results TLC spots were clear, the linear relationship between Paeoniflorin was good in the range of 0.06～1.8 μg(r=0.9999), the average recovery rate was 97.98%(n=6), and the RSD was 1.86%. Method Precision, repeatability meet the requirements. Conclusion The results of this method are accurate and reliable, and it is suitable for the quality control of Ganoderma lucidum liver pill.
Objective To establish the quality standard of Ganoderma lucidum liver pill. Methods Lingling, rhubarb, radix paeoniae, Huanglian, Magnolia and licorice were identified by microscopic identification, and rhubarb and Huanglian were identified by TLC, and the content of Paeoniflorin in Radix paeoniae was determined by high performance liquid chromatography.Results TLC spots were clear, the linear relationship between Paeoniflorin was good in the range of 0.06～1.8 μg(r=0.9999), the average recovery rate was 97.98%(n=6), and the RSD was 1.86%. Method Precision, repeatability meet the requirements. Conclusion The results of this method are accurate and reliable, and it is suitable for the quality control of Ganoderma lucidum liver pill.
引文
[1]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2015.
[2]叶强,余葱葱.大黄全成分薄层色谱条件研究[J].亚太传统医药,2013,9(3):15-16.
[3]王晓玲.大黄及其制剂的薄层色谱鉴别[J].中成药,2004,26(9):19-20.
[4]李文玮,刘东文.黄连泻心颗粒的薄层色谱鉴别研究[J].国际医药卫生导报,2008,14(16):84-85.
[5]杨小月,申欣.疏糖瘀胶囊中西洋参、黄芪、黄连的薄层色谱鉴别[J].中国中医急症杂志,2007,16(14):39-39.
[6]马冰,王晶,刘春明,等.不同提取方法对赤芍中芍药苷含量影响的高效液相色谱法对比研究[J].时珍国医国药,2013.24(8):1820-1822.
[7]杨敏聪.高效液相色谱法测定白带丸中芍药苷的含量[J].海峡药学,2007,19(6):54-56.
[8]姜晓燕,罗琳,窦志华,等.HPLC法测定赤芍饮片中芍药内酯苷及芍药苷的含量[J].齐鲁药事,2008,27(3):147-149.
[9]柳克浩.平痒洗剂质量标准研究[J].亚太传统医药,2017,13(4):23-26.
[2]叶强,余葱葱.大黄全成分薄层色谱条件研究[J].亚太传统医药,2013,9(3):15-16.
[3]王晓玲.大黄及其制剂的薄层色谱鉴别[J].中成药,2004,26(9):19-20.
[4]李文玮,刘东文.黄连泻心颗粒的薄层色谱鉴别研究[J].国际医药卫生导报,2008,14(16):84-85.
[5]杨小月,申欣.疏糖瘀胶囊中西洋参、黄芪、黄连的薄层色谱鉴别[J].中国中医急症杂志,2007,16(14):39-39.
[6]马冰,王晶,刘春明,等.不同提取方法对赤芍中芍药苷含量影响的高效液相色谱法对比研究[J].时珍国医国药,2013.24(8):1820-1822.
[7]杨敏聪.高效液相色谱法测定白带丸中芍药苷的含量[J].海峡药学,2007,19(6):54-56.
[8]姜晓燕,罗琳,窦志华,等.HPLC法测定赤芍饮片中芍药内酯苷及芍药苷的含量[J].齐鲁药事,2008,27(3):147-149.
[9]柳克浩.平痒洗剂质量标准研究[J].亚太传统医药,2017,13(4):23-26.