碱性蛋白酶和木聚糖酶对玉米-豆粕型日粮干物质和粗蛋白质的体外酶解效果研究
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摘要
本试验旨在通过透析袋体外酶解法研究碱性蛋白酶和木聚糖酶对玉米-豆粕型日粮还原糖生成量、干物质和粗蛋白质体外酶解效果的影响。试验采用单因子试验设计,碱性蛋白酶的添加浓度分别为20、40、60、80、100 U/g,木聚糖酶的添加浓度分别为10、20、30、40、50 U/g,每个添加水平分别设5个重复。结果表明:碱性蛋白酶添加浓度由20 U/g提高至100 U/g时,可显著提高日粮还原糖生成量和干物质酶解效率(二次,P<0.05);木聚糖酶添加浓度由10 U/g提高至50 U/g时,显著提高日粮干物质酶解效率(二次,P<0.05)。以干物质消化率为评价指标时,根据二次曲线方程计算得出碱性蛋白酶和木聚糖酶的适宜添加浓度分别为65 U/g和33 U/g。
This experiment was conducted to investigate the effects of alkaline protease and xylanase on the corn-soybean meal based diet using the in vitro enzymolysis efficiency of dry matter and crude protein, reducing sugar production as the response criteria. The different levels of protease(20, 40, 60, 80 and 100 U/g) and xylanase(10, 20, 30, 40 and 50 U/g) were added by one factor complete randomized experiment design to determine the optimal supplemental levels.The results showed that, increasing the concentration of alkaline protease from 20 to 100 U/g increased the amount of reducing sugar and also improved the in vitro enzymolysis efficiency of dry matter of the diet(quadratic effect, P<0.05); increasing the concentration of xylanase from 10 to 50 U/g increased the in vitro enzymolysis efficiency of dry matter(quadratic effect, P<0.05); when using the in vitro enzymolysis efficiency of dry matter as the evaluation index, the optimal supplemental levels of alkaline protease and xylanase were calculated as 65 U/g and 33 U/g, respectively.
This experiment was conducted to investigate the effects of alkaline protease and xylanase on the corn-soybean meal based diet using the in vitro enzymolysis efficiency of dry matter and crude protein, reducing sugar production as the response criteria. The different levels of protease(20, 40, 60, 80 and 100 U/g) and xylanase(10, 20, 30, 40 and 50 U/g) were added by one factor complete randomized experiment design to determine the optimal supplemental levels.The results showed that, increasing the concentration of alkaline protease from 20 to 100 U/g increased the amount of reducing sugar and also improved the in vitro enzymolysis efficiency of dry matter of the diet(quadratic effect, P<0.05); increasing the concentration of xylanase from 10 to 50 U/g increased the in vitro enzymolysis efficiency of dry matter(quadratic effect, P<0.05); when using the in vitro enzymolysis efficiency of dry matter as the evaluation index, the optimal supplemental levels of alkaline protease and xylanase were calculated as 65 U/g and 33 U/g, respectively.
引文
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[9]胡向东,焦乐飞,李旭彬,等.小麦代替玉米饲粮添加木聚糖酶对生长猪生长性能、结肠菌群和氮排放的影响[J].动物营养学报,2014,26(9):2805-2813.
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[11]薛梅,史雪萍,张廷荣,等.肉鸡小麦型饲粮4种单酶复配效应的体外法评定[J].动物营养学报,2014,26(12):3747-3756.
[12]张宏福,赵峰,张子仪.仿生消化法评定猪饲料生物学效价的研究进展[J].饲料与畜牧,2011(3):5-9.
[13]何科林,萨仁娜,高杰,等.体外法优化肉鸡日粮非淀粉多糖酶[J].中国农业科学,2012,45(21):4457-4464.
[14]Bedford M R,Classen H L.An in vitro assay for prediction of broiler intestinal viscosity and growth when fed rye-based diets in the presence of exogenous enzymes[J].Poult Sci,1993,72(1):137-143.
[15]高理想,陈亮,崔世贵,等.体外模拟消化法优化生长猪饲粮非淀粉多糖酶谱[J].动物营养学报,2017,29(4):1205-1217.
[16]Regmi P R,Ferguson N S,Zijlstra R T.In vitro digestibility techniques to predict apparent total tract energy digestibility of wheat in grower pigs[J].J Anim Sci,2009,87(11):3620-3629.
[2]杜忍让,张强,卢平祥,等.饲料中添加不同水平复合酶制剂对仔猪生长发育的影响[J].西北农业学报,2009,18(2):24-27.
[3]杜彦龙,周莉芬,袁绍辉,等.根源蛋白酶对不同类型日粮体外消化效果的研究[J].中国畜牧杂志,2016,52(6):46-50.
[4]吕景智.复合酶制剂体外酶解小麦的研究[J].饲料研究,2006(12):6-8.
[5]邓菊云.微生物碱性蛋白酶研究进展[J].现代食品科技,2008,24(3):293-296.
[6]杨胜远,陆兆新.微生物低温碱性蛋白酶的研究进展[J].食品与发酵工业,2004,30(4):93-98.
[7]万红贵,王涛,蔡恒,等.木聚糖酶的特性及应用研究[J].食品与发酵工业,2008,34(3):92-95.
[8]叶楠,陈代文,毛湘冰,等.不同木聚糖水平饲粮中添加木聚糖酶对断奶仔猪生长性能及肠道微生态环境的影响[J].动物营养学报,2011,23(11):1961-1969.
[9]胡向东,焦乐飞,李旭彬,等.小麦代替玉米饲粮添加木聚糖酶对生长猪生长性能、结肠菌群和氮排放的影响[J].动物营养学报,2014,26(9):2805-2813.
[10]Malathi V,Devegowda G.In vitro evaluation of nonstarch polysaccharide digestibility of feed ingredients by enzymes[J].Poult Sci,2001,80(3):302-305.
[11]薛梅,史雪萍,张廷荣,等.肉鸡小麦型饲粮4种单酶复配效应的体外法评定[J].动物营养学报,2014,26(12):3747-3756.
[12]张宏福,赵峰,张子仪.仿生消化法评定猪饲料生物学效价的研究进展[J].饲料与畜牧,2011(3):5-9.
[13]何科林,萨仁娜,高杰,等.体外法优化肉鸡日粮非淀粉多糖酶[J].中国农业科学,2012,45(21):4457-4464.
[14]Bedford M R,Classen H L.An in vitro assay for prediction of broiler intestinal viscosity and growth when fed rye-based diets in the presence of exogenous enzymes[J].Poult Sci,1993,72(1):137-143.
[15]高理想,陈亮,崔世贵,等.体外模拟消化法优化生长猪饲粮非淀粉多糖酶谱[J].动物营养学报,2017,29(4):1205-1217.
[16]Regmi P R,Ferguson N S,Zijlstra R T.In vitro digestibility techniques to predict apparent total tract energy digestibility of wheat in grower pigs[J].J Anim Sci,2009,87(11):3620-3629.