2015-2017年河北省邯郸市献血者HIV检测与确证分析
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摘要
目的了解邯郸市无偿献血者中的人类免疫缺陷病毒(HIV)感染率,分析HIV检测初筛与确证试验结果的相关性。方法对2015-2017年邯郸市中心血站的无偿献血者初筛有反应性的血液标本送HIV确证实验室进行确证,并进行初筛结果按S/CO值区间分组进行回顾性分析,与确证结果进行比较。结果研究期间259 650份标本中,有182例HIV初筛阳性,试剂1单试剂阳性53份,占初筛阳性总数的29. 12%,试剂2单试剂阳性87份,占初筛阳性总数的47. 80%; ELISA双试剂阳性42份,占初筛阳性总数的23. 08%;确证阳性30份全部来自双试剂阳性标本,确证阳性率为1. 16/万,确证阳性的标本分别分布在试剂1 S/CO值7. 0以上和试剂2 S/CO值10. 0以上。结论 ELISA双试剂阳性和确认阳性之间存在着明显的正相关关系,ELISA双试剂阳性与确证阳性有较高的吻合性。
Objective To analyze the rate of human immunodeficiency virus(HIV) infection among unpaid blood donors in Handan City,and to analyze the correlation between HIV screening and confirm test results. Methods The blood samples from the unpaid blood donors of the blood bank in the city center of 2015-2017 were sent to the HIV confirmation laboratory for confirmation and preliminary screening,and the preliminary screening results were retrospectively analyzed by S/CO interval,and compare with the confirmation result. Results Among the 259 650 specimens during the study period,182 were positive for HIV screening,53 were positive for reagent 1 single reagent,accounting for 29. 12% of the total number of positive screenings,and87 were positive for reagent 2 single reagents,accounting for 47. 80% of the total number of positive screenings; 42 positive ELISA two-reagent,accounting for 23. 08% of the total number of positive screenings; 30 positive samples were all from positive samples with double reagents,and the positive rate was 1. 16/10 000. The positive samples were distributed at the reagent1 S/CO value of 7. 0 or higher. And reagent 2 S/CO value of 10. 0 or more. Conclusion There was a significant positive correlation between positive double ELISA reagent and confirmed positivity,and there was a high coincidence between positive double ELISA reagent and confirmed positivity.
Objective To analyze the rate of human immunodeficiency virus(HIV) infection among unpaid blood donors in Handan City,and to analyze the correlation between HIV screening and confirm test results. Methods The blood samples from the unpaid blood donors of the blood bank in the city center of 2015-2017 were sent to the HIV confirmation laboratory for confirmation and preliminary screening,and the preliminary screening results were retrospectively analyzed by S/CO interval,and compare with the confirmation result. Results Among the 259 650 specimens during the study period,182 were positive for HIV screening,53 were positive for reagent 1 single reagent,accounting for 29. 12% of the total number of positive screenings,and87 were positive for reagent 2 single reagents,accounting for 47. 80% of the total number of positive screenings; 42 positive ELISA two-reagent,accounting for 23. 08% of the total number of positive screenings; 30 positive samples were all from positive samples with double reagents,and the positive rate was 1. 16/10 000. The positive samples were distributed at the reagent1 S/CO value of 7. 0 or higher. And reagent 2 S/CO value of 10. 0 or more. Conclusion There was a significant positive correlation between positive double ELISA reagent and confirmed positivity,and there was a high coincidence between positive double ELISA reagent and confirmed positivity.
引文
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[11] BUSCH MP. HIV,HBV,and HCV:new developments related to transfusion safety[J]. Vox Sang,2000,78(S12):253-256.
[12]谢敬文,蓝文莉,黎淑贞,等.番禺地区无偿献血者酶免阴性样本的核酸检测结果分析[J].临床输血与检验,2014,16(4):355-357.
[13]王芳,栾燕,刘显智.核酸检测技术(NAT)在献血者人类免疫缺陷病毒筛查中的应用[J].中国输血杂志,2010,23(10):892-894.
[14]殷崎涛,钱惠忠,李玉琪,等. 1例HIV低病毒载量窗口期样本的发现及对降低血液残余风险的思考[J].临床输血与检验,2016,18(4):335-337.
[15]徐志华,黄宏亮,姜斌,等. 48754份无偿献血者血液标本核酸检测技术筛查情况分析[J].中国输血杂志,2015,28(8):908-910.
[2]黄秀琳,冷文杰,尹丹,等.重庆市无偿献血者HIV筛查及确认结果对比分析[J].中国输血杂志,2015,28(9):1128-1130.
[3]杨忠思,冯秋霞,侯云,等.青岛地区无偿献血人群HIV筛查结果回顾性分析[J].中国输血杂志,2015,28(4):428-430.
[4]王东,邓雪莲,臧亮,等.大连地区无偿献血者人群中HIV感染情况调查[J].中国输血杂志,2013,26(8):746-747.
[5]魏淑梅,孙国栋,左志平,等. 2002-2013年邯郸市无偿献血人群HIV筛查及确证情况分析[J].中国输血杂志,2014,27(8):856-858.
[6]熊志高,刘昕晨,谭艳阳.株洲市无偿献血者HIV感染率及初筛与确证试验结果分析[J].中国输血杂志,2016,29(4):406-408.
[7]魏兰华. ELISA法检测抗-HIV实验中灰区设置探讨[J].临床输血与检验,2015,16(6):566-681.
[8]郭巍,张婷.献血者HIV筛查结果的回顾性分析及灰区设置的探讨[J].临床输血与检验,2017,19(3):281-283.
[9] BAGHERI R,RABBANI B,MAHDIEH N,et al. PCR-ELISA:diagnosticassay for identifying Iranian HIV sreopositives1[J]. Mol Gen Microbiol Virusol,2013,28(3):36-39.
[10] TIWARI RP,JAIN A,KHAN Z,et al. Designing of novel antigenic peptide cocktail for the detection of antibodies to HIV-1/2b by ELISA[J]. J Immunol Methods,2013,387(1/2):157-166.
[11] BUSCH MP. HIV,HBV,and HCV:new developments related to transfusion safety[J]. Vox Sang,2000,78(S12):253-256.
[12]谢敬文,蓝文莉,黎淑贞,等.番禺地区无偿献血者酶免阴性样本的核酸检测结果分析[J].临床输血与检验,2014,16(4):355-357.
[13]王芳,栾燕,刘显智.核酸检测技术(NAT)在献血者人类免疫缺陷病毒筛查中的应用[J].中国输血杂志,2010,23(10):892-894.
[14]殷崎涛,钱惠忠,李玉琪,等. 1例HIV低病毒载量窗口期样本的发现及对降低血液残余风险的思考[J].临床输血与检验,2016,18(4):335-337.
[15]徐志华,黄宏亮,姜斌,等. 48754份无偿献血者血液标本核酸检测技术筛查情况分析[J].中国输血杂志,2015,28(8):908-910.