青杨雄株组织培养技术研究
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摘要
以青杨雄株1号和2号一年生健壮枝条为外植体,比较不同激素浓度和配比对诱导培养、增殖培养、生根培养及炼苗成活率的影响。结果表明:最佳诱导培养基为MS+6-BA0.3+NAA0.05(单位:mg/l,下同),最佳增殖培养基为MS,最佳生根培养1/2MS+NAA0.1,最佳炼苗基质为进口基质+渣筏(2∶1)。
Taking the robust annual branches of Populus cathayana as explants,the effects of different hormone concentrations and ratios on induction culture,proliferation culture,rooting culture and hardening seedling survival rate were compared. The results showed that the best induction medium is MS + 6-BA0. 3+ NAA0. 05,the optimal proliferation medium is MS,the best rooting culture is 1/2 MS + NAA 0. 1,the best seedling hardening substrate is import matrix + peat soil( 2∶ 1).
Taking the robust annual branches of Populus cathayana as explants,the effects of different hormone concentrations and ratios on induction culture,proliferation culture,rooting culture and hardening seedling survival rate were compared. The results showed that the best induction medium is MS + 6-BA0. 3+ NAA0. 05,the optimal proliferation medium is MS,the best rooting culture is 1/2 MS + NAA 0. 1,the best seedling hardening substrate is import matrix + peat soil( 2∶ 1).
引文
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[5]林丽飞,夏瑞娥,刘春国,等.不同因素对短葶飞蓬愈伤组织的影响[J].江苏农业科学,2013,41(2):52-54.
[6]李宗霆,周燮.植物激素及其免疫检测技术[M].南京:江苏科学技术出版社,1996.
[2]余桂红,张旭,孙晓波,等.大麦苏啤4号幼胚愈伤组织的诱导及植株的高频再生[J].江苏农业学报,2013,29(5):953-956.
[3]Narayanan S L,lbrahim S M,Ashok S,et al.Effect of abscisic acid in enhancing plant regenerability in rice[J].Rice Biotechnology Quarterly,2000,41(4):10-16.
[4]陈豫,胡伟,何磊.不同浓度激素对胡萝愈伤组织诱导的影响[J].江苏农业科学,2013,41(2):54-56.
[5]林丽飞,夏瑞娥,刘春国,等.不同因素对短葶飞蓬愈伤组织的影响[J].江苏农业科学,2013,41(2):52-54.
[6]李宗霆,周燮.植物激素及其免疫检测技术[M].南京:江苏科学技术出版社,1996.