山药块茎膨大过程内源激素的变化及相关基因的表达验证
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摘要
山药(Dioscorea opposite, 2n=40)属于薯蓣科,其地下茎能膨大,膨大的地下茎具有良好的食用和药用价值,为了解析山药块茎膨大的分子机制,本研究对块茎膨大的3个时期对其叶片、地上茎、块茎各部位进行取样,测定内源激素含量,并结合山药转录组数据,对植物激素相关基因进行表达验证。研究表明:IAA、JA、MeJA全部呈上升趋势,而GA3含量先上升后下降;IAA相关基因SAUR (Unigene8864_All)和PIN domain-containing protein (CL3538.Contig1_All)在块茎膨大过程起促进作用;JA相关基因Allene oxide synthase (CL3684.Contig3_All)起促进作用。研究表明山药块茎膨大与植物激素息息相关,为深入研究山药块茎膨大进程,提高山药产量和质量提供了理论依据。
Yam(Dioscorea opposite 2 n=40) belongs to Dioscorea family. Its underground stem can enlarge, which has good edible and medicinal value. In order to understand the molecular mechanism of yam tuber enlargement,this study sampled the leaves, aboveground stems and tubers at three stages of tuber enlargement, determined the level of endogenous hormones, and combined with the transcriptome data of yam to verify the related genes expression of plant hormones. The research showed IAA, JA and Me JA all tend to rise up while GA3 increased first and then decreased. IAA-related genes SAUR(Unigene 8864_All) and PIN domain-containing protein(CL35-38.Contig1_All) play a role in promoting tuber expansion. JA related gene allene oxide synthase(CL3684.Contig3_All) plays a promotive role. These results indicated that the expansion of yam tubers was highly related to plant hormones, which provided a theoretical evidence for further studying the process of tuber expansion and improving the yield and quality of yam.
Yam(Dioscorea opposite 2 n=40) belongs to Dioscorea family. Its underground stem can enlarge, which has good edible and medicinal value. In order to understand the molecular mechanism of yam tuber enlargement,this study sampled the leaves, aboveground stems and tubers at three stages of tuber enlargement, determined the level of endogenous hormones, and combined with the transcriptome data of yam to verify the related genes expression of plant hormones. The research showed IAA, JA and Me JA all tend to rise up while GA3 increased first and then decreased. IAA-related genes SAUR(Unigene 8864_All) and PIN domain-containing protein(CL35-38.Contig1_All) play a role in promoting tuber expansion. JA related gene allene oxide synthase(CL3684.Contig3_All) plays a promotive role. These results indicated that the expansion of yam tubers was highly related to plant hormones, which provided a theoretical evidence for further studying the process of tuber expansion and improving the yield and quality of yam.
引文
Alizad eh S.,Mantell S.H.,and Mariaviana A.,1998,In vitro shoot culture and microtuber induction in the steroid yam Dioscorea composita Hemsl,Plant Cell Tissue&Organ Culture,53(2):107-112
Chang L.,Xue J.P.,Song Y.X.,Sheng W.,and Xiong C.H.,2010,Variation of endogenous hormones in formation of microtuber of Dioscorea opposite in vitro,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materia Medica),35(21):2818-2821(常莉,薛建平,宋运贤,盛玮,熊聪慧,2010,怀山药试管块茎形成过程中内源激素的变化,中国中药杂志,35(21):2818-2821)
Hu J.B.,Li J.W.,and Sun S.R.,2007,Extraction and high performance liquid chromatographic determination of endogenous hormones in Dioscorea zingiberensis C.H.Wright,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),43(3):529-532(胡建斌,李建吾,孙守如,2007,薯蓣中内源激素的提取及高效液相色谱测定法,植物生理学通讯,43(3):529-532)
Hu Y.H.,and Jiang X.M.,1992,The Effects of plant hormones on tuberization of potato microtubers in vitro,Zhongguo Malingshu(Chinese Potato),6(1):14-22(胡云海,蒋先明,1992,植物激素对微型薯形成的影响,中国马铃薯,6(1):14-22)
Koops P.,Pelser S.,Ignatz M.,Klose C.,Marrocco-Selden K.,and Kretsch T.,2011,EDL3 is an F-box protein involved in the regulation of abscisic acid signalling in Arabidopsis thaliana,J.Exp.Bot.,62(15):5547-5560
Li M.J.,Liu X.Y.,Li P.,Zhang X.L.,Zhao X.T.,Liu J.,and Xie C.H.,2008,Impact factors in in vitro induction of microtubers from Dioscorea opposite,Chinese Traditional&Herbal Drugs,39(6):905-910
Liang R.F.,Li C.Z.,Zhang J.,He L.F.,Wei B.H.,Gan X.Q.,and He H.Y.,2011,Changes of matter accumulation and relative enzymatic activity during yam tuber development,Zuowu Xuebao(Acta Agronomica Sinica),37(5):903-910(梁任繁,李创珍,张娟,何龙飞,韦本辉,甘秀芹,何虎翼,2011,山药块茎发育中物质积累及相关代谢酶变化,作物学报,37(5):903-910)
Liu M.Y.,Mao X.F.,Men F.Y.,Meng M.L.,and Hu Z.Q.,1997,Correlation law between the change of intrinsic hormones of potato tuber and growth of the tuber,Huabei Nongxuebao(Acta Agriculturae Boreall-Sinica),12(2):86-92(刘梦云,毛雪飞,门福义,蒙美莲,胡志全,1997,马铃薯块茎内源激素变化与块茎增大生长的相关规律,华北农学报,12(2):86-92)
Meng M.L.,Liu M.Y.,Men F.Y.,and Chen Y.J.,1996,Study on the content of internal GA3and ABA in growing potato tuber,Zhongguo Malingshu(Chinese Potato Journal),10(1):8-12(蒙美莲,刘梦芸,门福义,陈有君,1996,马铃薯块茎生长过程中内源赤霉素和脱落酸含量的变化,中国马铃薯,10(1):8-12)
Sarkar D.,Pandey S.K.,and Sharma S.,2006,Cytokinins antagonize the jasmonates action on the regulation of potato(Solanum tuberosum)tuber formation in vitro,Plant Cell,Tissue and Organ Culture,87(3):285-295
Sheng W.,Xue J.P.,Zhang A.M.,Chang L.,and Fan C.C.,2010,Variation of endogenous hormones information of microtubers of Pinellia ternata in vitro,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materia Medica),35(8):943-946(盛玮,薛建平,张爱民,常莉,范成成,2010,半夏试管块茎形成过程中内源激素的变化,中国中药杂志,35(8):943-946)
Yu S.N.,and Cui J.Z.,2009,Role of PIN proteins in polar auxin transport,Shengwu Jishu Tongbao(Biotechnology Bulletin),(3):20-24(于胜楠,崔继哲,2009,PIN蛋白在生长素极性运输中的作用,生物技术通报,(3):20-24)
Yuan S.L.,2008,Research advances on chemical compositions and bioactivities of Dioscorea opposita thunb.(chinese yam),Food Research&Development,29(3):176-179
Zhao B.,Zhang C.R.,Li J.M.,and Wu G.Q.,2003,The Comparative study on the pipe culture technique and half-pipe culture technique of Dioscorea opposita thunb,Special Wild E-conomic Animal&Plant Research,(4):19-21
Zhu Y.B.,Kong Y.Y.,and Wang J.H.,2014,Research advances in auxin-responsive SAUR genes,Shengming Kexue(Chinese Bulletin of Life Sciences),26(4):407-413(朱宇斌,孔莹莹,王君晖,2014,植物生长素响应基因SAUR的研究进展,生命科学,26(4):407-413)
Chang L.,Xue J.P.,Song Y.X.,Sheng W.,and Xiong C.H.,2010,Variation of endogenous hormones in formation of microtuber of Dioscorea opposite in vitro,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materia Medica),35(21):2818-2821(常莉,薛建平,宋运贤,盛玮,熊聪慧,2010,怀山药试管块茎形成过程中内源激素的变化,中国中药杂志,35(21):2818-2821)
Hu J.B.,Li J.W.,and Sun S.R.,2007,Extraction and high performance liquid chromatographic determination of endogenous hormones in Dioscorea zingiberensis C.H.Wright,Zhiwu Shenglixue Tongxun(Plant Physiology Communications),43(3):529-532(胡建斌,李建吾,孙守如,2007,薯蓣中内源激素的提取及高效液相色谱测定法,植物生理学通讯,43(3):529-532)
Hu Y.H.,and Jiang X.M.,1992,The Effects of plant hormones on tuberization of potato microtubers in vitro,Zhongguo Malingshu(Chinese Potato),6(1):14-22(胡云海,蒋先明,1992,植物激素对微型薯形成的影响,中国马铃薯,6(1):14-22)
Koops P.,Pelser S.,Ignatz M.,Klose C.,Marrocco-Selden K.,and Kretsch T.,2011,EDL3 is an F-box protein involved in the regulation of abscisic acid signalling in Arabidopsis thaliana,J.Exp.Bot.,62(15):5547-5560
Li M.J.,Liu X.Y.,Li P.,Zhang X.L.,Zhao X.T.,Liu J.,and Xie C.H.,2008,Impact factors in in vitro induction of microtubers from Dioscorea opposite,Chinese Traditional&Herbal Drugs,39(6):905-910
Liang R.F.,Li C.Z.,Zhang J.,He L.F.,Wei B.H.,Gan X.Q.,and He H.Y.,2011,Changes of matter accumulation and relative enzymatic activity during yam tuber development,Zuowu Xuebao(Acta Agronomica Sinica),37(5):903-910(梁任繁,李创珍,张娟,何龙飞,韦本辉,甘秀芹,何虎翼,2011,山药块茎发育中物质积累及相关代谢酶变化,作物学报,37(5):903-910)
Liu M.Y.,Mao X.F.,Men F.Y.,Meng M.L.,and Hu Z.Q.,1997,Correlation law between the change of intrinsic hormones of potato tuber and growth of the tuber,Huabei Nongxuebao(Acta Agriculturae Boreall-Sinica),12(2):86-92(刘梦云,毛雪飞,门福义,蒙美莲,胡志全,1997,马铃薯块茎内源激素变化与块茎增大生长的相关规律,华北农学报,12(2):86-92)
Meng M.L.,Liu M.Y.,Men F.Y.,and Chen Y.J.,1996,Study on the content of internal GA3and ABA in growing potato tuber,Zhongguo Malingshu(Chinese Potato Journal),10(1):8-12(蒙美莲,刘梦芸,门福义,陈有君,1996,马铃薯块茎生长过程中内源赤霉素和脱落酸含量的变化,中国马铃薯,10(1):8-12)
Sarkar D.,Pandey S.K.,and Sharma S.,2006,Cytokinins antagonize the jasmonates action on the regulation of potato(Solanum tuberosum)tuber formation in vitro,Plant Cell,Tissue and Organ Culture,87(3):285-295
Sheng W.,Xue J.P.,Zhang A.M.,Chang L.,and Fan C.C.,2010,Variation of endogenous hormones information of microtubers of Pinellia ternata in vitro,Zhongguo Zhongyao Zazhi(China Journal of Chinese Materia Medica),35(8):943-946(盛玮,薛建平,张爱民,常莉,范成成,2010,半夏试管块茎形成过程中内源激素的变化,中国中药杂志,35(8):943-946)
Yu S.N.,and Cui J.Z.,2009,Role of PIN proteins in polar auxin transport,Shengwu Jishu Tongbao(Biotechnology Bulletin),(3):20-24(于胜楠,崔继哲,2009,PIN蛋白在生长素极性运输中的作用,生物技术通报,(3):20-24)
Yuan S.L.,2008,Research advances on chemical compositions and bioactivities of Dioscorea opposita thunb.(chinese yam),Food Research&Development,29(3):176-179
Zhao B.,Zhang C.R.,Li J.M.,and Wu G.Q.,2003,The Comparative study on the pipe culture technique and half-pipe culture technique of Dioscorea opposita thunb,Special Wild E-conomic Animal&Plant Research,(4):19-21
Zhu Y.B.,Kong Y.Y.,and Wang J.H.,2014,Research advances in auxin-responsive SAUR genes,Shengming Kexue(Chinese Bulletin of Life Sciences),26(4):407-413(朱宇斌,孔莹莹,王君晖,2014,植物生长素响应基因SAUR的研究进展,生命科学,26(4):407-413)