生鲜牛乳中三种β-内酰胺酶检测方法的比较
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摘要
对3种实验室常用的β-内酰胺酶检测方法(杯碟法、碘量法、Charm MRL快速检测法)进行了比较,获得了3种方法对β-内酰胺酶的检出限,并提出了方法中应该注意的关键点,以确保实验效果。从原理、仪器设备、检验准备、检验操作、检验周期、检验成本等方面进行了比较,对3种方法的在不同情况下的适用性提出了建议。
Cylinder plate method, iodimetry, Charm MRL rapid test method were compared, which were used to detect β-lactamase in Raw Milk, the detection limits were obtained, the key points that should be paid attention were given to ensure the experimental results.Three methods were compared from principle, equipment, preparation, operation, period, cost and so on. The applicability of the three methods under different conditions were Suggested.
Cylinder plate method, iodimetry, Charm MRL rapid test method were compared, which were used to detect β-lactamase in Raw Milk, the detection limits were obtained, the key points that should be paid attention were given to ensure the experimental results.Three methods were compared from principle, equipment, preparation, operation, period, cost and so on. The applicability of the three methods under different conditions were Suggested.
引文
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[5]胡晓宁,苏诚玉,权玉玲,等.甘肃省生鲜乳中β-内酰胺酶含量调查分析[J].疾病预防控制通报,2013(5):79-80.
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[8]帕提古丽·托乎提,Patiguli·Tuohuti.新疆巴州地区生鲜牛乳中β-内酰胺酶残留状况调查[J].中国动物检疫,2015(10):19-20.
[9]谢岩黎,张鑫潇,严瑞东,等.碘量法对乳制品中β-内酰胺酶的检测[J].中国乳品工业,2010,38(12):36-37.
[10]刘珊珊,周正,周巍,等.酸度计法快速检测牛奶中残留的β-内酰胺酶[J].食品科学,2010,31(10):216-218.
[11]林钦.高效液相色谱法同时检测乳制品中3种β-内酰胺酶抑制剂的残留量[J].色谱,2013,31(5):441-446.
[12]沙芳芳,刘卫华,王敬,等.牛奶中β-内酰胺酶抑制剂—舒巴坦直接竞争ELISA的分析研究[J].食品工业科技,2017,38(10).
[13]李欣南,韩镌竹,苏崴艺.生鲜乳中β-内酰胺酶微生物法检测[J].中国乳品工业,2015,43(3):57-59.
[14]山瑛.关于《乳与乳制品中舒巴坦β-内酰胺酶类药物检验方法-杯碟法》的优化与探讨[J].中国卫生检验杂志,2011(4):1033-1033.
[15]马玉华.关于《乳与乳制品中舒巴坦敏感β-内酰胺酶类药物检测方法—杯碟法》的经验探讨[J].中国卫生检验杂志,2010(10):2621-2621.
[16]薛晓晶,李玲,金涌,等.杯碟法检测乳中的β-内酰胺酶[J].食品科学,2011,32(4):216-219.
[17]康海英,王加启,卜登攀,等.两种检测生鲜乳中β-内酰胺酶方法的比较[J].生物技术通报,2010(6):202-205.
[2]周蕊,丁颢,周爽,等.我国部分地区市售巴氏杀菌乳中β-内酰胺酶含量调查[J].中国食品卫生杂志,2014,26(3):209-212.
[3]秦婧,张莉,舒静,等.陕西省生鲜乳中β-内酰胺酶的检测与结果分析[J].中国乳品工业,2016,44(9):61-64.
[4]沈赟,符晓梅,乔昕,等.南京市乳制品中β-内酰胺酶类药物残留状况调查[J].江苏预防医学,2010,21(4):6-8.
[5]胡晓宁,苏诚玉,权玉玲,等.甘肃省生鲜乳中β-内酰胺酶含量调查分析[J].疾病预防控制通报,2013(5):79-80.
[6]马景宏,张旭,赵虹,等.沈阳市售乳制品中β-内酰胺酶残留状况初探[J].中国微生态学杂志,2012,24(7):606-608.
[7]侯海燕,刘靓,刘纯成,等.淮安市市售乳制品中β-内酰胺酶残留状况调查[J].江苏预防医学,2015,26(6):92-93.
[8]帕提古丽·托乎提,Patiguli·Tuohuti.新疆巴州地区生鲜牛乳中β-内酰胺酶残留状况调查[J].中国动物检疫,2015(10):19-20.
[9]谢岩黎,张鑫潇,严瑞东,等.碘量法对乳制品中β-内酰胺酶的检测[J].中国乳品工业,2010,38(12):36-37.
[10]刘珊珊,周正,周巍,等.酸度计法快速检测牛奶中残留的β-内酰胺酶[J].食品科学,2010,31(10):216-218.
[11]林钦.高效液相色谱法同时检测乳制品中3种β-内酰胺酶抑制剂的残留量[J].色谱,2013,31(5):441-446.
[12]沙芳芳,刘卫华,王敬,等.牛奶中β-内酰胺酶抑制剂—舒巴坦直接竞争ELISA的分析研究[J].食品工业科技,2017,38(10).
[13]李欣南,韩镌竹,苏崴艺.生鲜乳中β-内酰胺酶微生物法检测[J].中国乳品工业,2015,43(3):57-59.
[14]山瑛.关于《乳与乳制品中舒巴坦β-内酰胺酶类药物检验方法-杯碟法》的优化与探讨[J].中国卫生检验杂志,2011(4):1033-1033.
[15]马玉华.关于《乳与乳制品中舒巴坦敏感β-内酰胺酶类药物检测方法—杯碟法》的经验探讨[J].中国卫生检验杂志,2010(10):2621-2621.
[16]薛晓晶,李玲,金涌,等.杯碟法检测乳中的β-内酰胺酶[J].食品科学,2011,32(4):216-219.
[17]康海英,王加启,卜登攀,等.两种检测生鲜乳中β-内酰胺酶方法的比较[J].生物技术通报,2010(6):202-205.