不同生长年限川牛膝中葛根素和杯苋甾酮含量比较与分析研究
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摘要
目的比较不同生长年限川牛膝药材中葛根素和杯苋甾酮的含量差异,为其采收年限确定和质量控制提供依据。方法采用高效液相色谱法,色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),检测波长243nm,流动相0.1%磷酸溶液-乙腈,流速1.0 m L/min。结果不同生长年限川牛膝药材中杯苋甾酮的含量高低依次为:3年(0.84±0.14 mg/g)>2年(0.65±0.09 mg/g)>1年(0.46±0.08 mg/g);葛根素的含量高低依次为:3年(21.41±10.21μg/g)>2年(10.08±7.96μg/g)>1年(6.97±4.12μg/g)。结论不同生长年限的川牛膝药材中两种有效成分含量有明显的差异(P<0.05)。因此,应以3年生的川牛膝采收入药最佳。
Objective To compare the differences of puerarin and cyasterone in Cyathulae radix with different growth years, and provide a reference for the harvest age and quality control. Methods The samples were separated on a Kromasil C18 column(250 mm×4.6 mm, 5 μm). Acetonitrile-0.1 % phosphoric acid solution was used as mobile phase, with a flow rate of 1.0 m L/min. The detection wavelength was set at 243 nm. Results The contents of cyasterone in Cyathulae radix with different growth years were in the order: 3 years(0.84±0.14 mg/g) > 2 years(0.65±0.09 mg/g) > 1 year(0.46±0.08 mg/g), and the contents of puerarin were in the order: 3 years(21.41±10.21 μg/g) > 2 years(10.08±7.96 μg/g) > 1 year(6.97±4.12 μg/g). Conclusion The contents differences of the two effective components in Cyathulae radix with different growth years are significant(P<0.05). The results indicate that optimal harvest age of Cyathulae radix is 3 years.
Objective To compare the differences of puerarin and cyasterone in Cyathulae radix with different growth years, and provide a reference for the harvest age and quality control. Methods The samples were separated on a Kromasil C18 column(250 mm×4.6 mm, 5 μm). Acetonitrile-0.1 % phosphoric acid solution was used as mobile phase, with a flow rate of 1.0 m L/min. The detection wavelength was set at 243 nm. Results The contents of cyasterone in Cyathulae radix with different growth years were in the order: 3 years(0.84±0.14 mg/g) > 2 years(0.65±0.09 mg/g) > 1 year(0.46±0.08 mg/g), and the contents of puerarin were in the order: 3 years(21.41±10.21 μg/g) > 2 years(10.08±7.96 μg/g) > 1 year(6.97±4.12 μg/g). Conclusion The contents differences of the two effective components in Cyathulae radix with different growth years are significant(P<0.05). The results indicate that optimal harvest age of Cyathulae radix is 3 years.
引文
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[11]王怀玉,马鹏,彭锐.不同生长年限太白贝母中贝母辛和总生物碱的含量测定[J].中药材,2011,34(7):1034-1037.
[12]肖新月,尹继飞,张南平,等.不同生长年限的人参中8种主要皂苷成分的分析研究[J].药物分析杂志,2004,24(3):238-244.
[2]陈幸,黎万寿,梁溢,等.川牛膝化学成分的鉴定[J].中草药,2004,35(9):978-979.
[3]田会萍,董亚琳.川牛膝的药学研究进展[J].国际中医中药杂志,2013,35(3):270-273.
[4]周容.川牛膝及西南风车子的化学成分研究[D].中国科学院成都有机化学研究所,2004.
[5]谭玉柱,童婷婷,潘晓丽,等.川牛膝的HPLC指纹图谱研究[J].成都中医药大学学报,2011,4(3):75-77.
[6]陈幸,黎万寿,朱久武,等.RP-HPLC法测定川牛膝中杯苋甾酮的含量[J].药物分析杂志,2000,20(4):69-71.
[7]马英,毕开顺,王玺,等.HPLC法测定川牛膝中杯苋甾酮的含量[J].中草药,2000,31(6):427-428.
[8]张晓晓,刘胜,薛晓红.具有雌激素类似作用的中药研究进展[J].结合医学学报,2005,3(2):149-153.
[9]钟保恒.葛根素药理研究现状[J].中国民族民间医药,2009,18(5):16-18.
[10]杨鹏,李秀兰,贾雪岭.葛根素的药理作用和临床应用[J].内蒙古民族大学学报(自然科学版),2013,28(2):226-227.
[11]王怀玉,马鹏,彭锐.不同生长年限太白贝母中贝母辛和总生物碱的含量测定[J].中药材,2011,34(7):1034-1037.
[12]肖新月,尹继飞,张南平,等.不同生长年限的人参中8种主要皂苷成分的分析研究[J].药物分析杂志,2004,24(3):238-244.