基于AHP-CRITIC法的正交设计优选参膝口服液提取工艺
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摘要
目的确定指标权重,优选参膝口服液的最佳提取工艺。方法以HPLC法测定杯苋甾酮、人参皂苷Rg1、Re、Rb1的量,HPLC-ELSD法测定人参二醇的量、干膏率、总多糖含量及总峰面积作为评价指标,采用层次分析法(AHP)、基于指标相关性的权重确定方法(CRITIC)、AHP-CRITIC混合加权法确定各评价指标的权重系数,比较混合加权法和单一赋权法,并结合正交试验结果优选复方提取工艺的最佳参数。结果 AHP-CRITIC混合加权法较单一赋权法科学、合理、全面,按其所得的权重系数进行综合评价,优选出最佳工艺参数为复方药材加9倍量水,提取3次,每次80 min。3批验证试验综合评分均值为99.06,RSD为0.18%。结论 AHP法结合CRITIC法可用于参膝口服液提取工艺多指标权重的建立,优选的提取工艺经验证,合理、稳定,重复性好,可用于工业化生产。
Objective The extraction process of Shenxi Oral Liquid by exploring the optional extraction process parameters based on determining the index weight. Methods Using the contents of cyasterone, ginsenosides Rg1, Re, Rb1, panaxadiol, ratio of dry extraction, total polysaccharides, and total peak area as comprehensive evaluation indexes, the weighting coefficient in eight synthetic evaluation indexes was determined by analytic hierarchy process(AHP), criteria importance through intercriteria correlation(CRITIC) method, and mixed weighted AHP-CRITIC. Compared mixed weighting method and single weighting method, the optimal parameters of compound extraction process were optimized by orthogonal test. Results Mixed weighted AHP-CRITIC was more scientific, reasonable, and comprehensive than the single weighting method. According to the weighting coefficient determined by comprehensive evaluation, the optimal process parameters were as follow: compound herbs plus nine times of water, extracting three times, each for 80 min. The mean of three batches of comprehensive evaluation was 99.06 and RSD value was 0.18%. Conclusion AHP combined with CRITIC could be used to establish weighting coefficient of the compound formula extraction process and the optimized process of extraction had been verified to be reasonable, stable, and reproducible, which could be used for industrial production.
Objective The extraction process of Shenxi Oral Liquid by exploring the optional extraction process parameters based on determining the index weight. Methods Using the contents of cyasterone, ginsenosides Rg1, Re, Rb1, panaxadiol, ratio of dry extraction, total polysaccharides, and total peak area as comprehensive evaluation indexes, the weighting coefficient in eight synthetic evaluation indexes was determined by analytic hierarchy process(AHP), criteria importance through intercriteria correlation(CRITIC) method, and mixed weighted AHP-CRITIC. Compared mixed weighting method and single weighting method, the optimal parameters of compound extraction process were optimized by orthogonal test. Results Mixed weighted AHP-CRITIC was more scientific, reasonable, and comprehensive than the single weighting method. According to the weighting coefficient determined by comprehensive evaluation, the optimal process parameters were as follow: compound herbs plus nine times of water, extracting three times, each for 80 min. The mean of three batches of comprehensive evaluation was 99.06 and RSD value was 0.18%. Conclusion AHP combined with CRITIC could be used to establish weighting coefficient of the compound formula extraction process and the optimized process of extraction had been verified to be reasonable, stable, and reproducible, which could be used for industrial production.
引文
[1]王桂蓉,黄涛,李根.参杞口服液质量标准研究[A]//2008年成渝药学学术年会论文集[C].成都:成渝药学学术年会,2008.
[2]郭俊生,陈洪章,赵法伋,等.参杞口服液的抗衰老作用[J].中国老年学杂志,1996,16(1):24-27.
[3]宋佳,黄飞龙,段树卿,等.Plackett-Burnman设计联合Box-Behnken设计-效应面法优化心神宁片的提取工艺[J].中草药,2016,47(3):430-435.
[4]石浩源,王春雷,赵艳敏,等.正交试验结合体外抑制肠平滑肌收缩活性研究优化固公果根总三萜提取工艺[J].药物评价研究,2017,40(11):1581-1586.
[5]余姣,陈娇,周文杰,等.基于抗炎解热药效的小儿温热口服液中心复合设计-效应面法提取工艺优化研究[J].中草药,2016,47(20):3632-3638.
[6]殷明阳,刘毅,那溪元,等.正交试验优化止咳平喘颗粒处方药材的乙醇提取工艺[J].现代药物与临床,2015,30(10):1208-1211.
[7]周泽琴,蔡延渠,张雄飞,等.中药水提取有效成分转移率低的问题分析[J].中草药,2014,45(23):3478-3485.
[8]中国药典[S].一部.2015.
[9]杨铭,周昕,谢瑞方,等.用层次分析法结合CRITIC法研究复方自身清颗粒提取工艺的多指标权重[J].药学服务与研究,2009,9(1):36-39.
[10]杨秀青,石征蓉,谷江华,等.银菊解毒口服液大孔树脂纯化工艺研究[J].中草药,2017,48(23):4904-4911.
[11]石振武,赵敏.运用层次分析法确定指标的权值[J].科技和产业,2008,8(2):23-25.
[12]刘小妹,施崇精,程中琴,等.人参水解物中人参二醇的工艺优化及其HPLC-ELSD含量测定方法[J/OL].天然产物研究与开发,2018-04-18.
[13]林丽,王琰,王福星,等.基于硫酸-蒽酮法对不同产地藏药线叶龙胆多糖的含量测定[J].中国中药杂志,2014,39(14):2774-2776.
[14]辛敏,刘轩,詹欣,等.硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较[J].中国实验方剂学杂志,2014,20(3):62-65.
[15]黄潇,刘婧,付小梅,等.基于CRITIC法计算权重系数的Box-Behnken响应面法优化栀子炭微波炮制工艺研究[J].中草药,2017,48(6):1133-1138.
[16]贾执瑛,谢燮,王晓艳,等.人参主要成分对大鼠免疫功能的比较研究[J].中国中药杂志,2014,39(17):3363-3366.
[17]郑智茵,尹利明,庄海峰,等.人参二醇组皂苷提取物对再生障碍性贫血小鼠免疫调节作用的研究[J].中国药理学通报,2015,31(6):790-795.
[2]郭俊生,陈洪章,赵法伋,等.参杞口服液的抗衰老作用[J].中国老年学杂志,1996,16(1):24-27.
[3]宋佳,黄飞龙,段树卿,等.Plackett-Burnman设计联合Box-Behnken设计-效应面法优化心神宁片的提取工艺[J].中草药,2016,47(3):430-435.
[4]石浩源,王春雷,赵艳敏,等.正交试验结合体外抑制肠平滑肌收缩活性研究优化固公果根总三萜提取工艺[J].药物评价研究,2017,40(11):1581-1586.
[5]余姣,陈娇,周文杰,等.基于抗炎解热药效的小儿温热口服液中心复合设计-效应面法提取工艺优化研究[J].中草药,2016,47(20):3632-3638.
[6]殷明阳,刘毅,那溪元,等.正交试验优化止咳平喘颗粒处方药材的乙醇提取工艺[J].现代药物与临床,2015,30(10):1208-1211.
[7]周泽琴,蔡延渠,张雄飞,等.中药水提取有效成分转移率低的问题分析[J].中草药,2014,45(23):3478-3485.
[8]中国药典[S].一部.2015.
[9]杨铭,周昕,谢瑞方,等.用层次分析法结合CRITIC法研究复方自身清颗粒提取工艺的多指标权重[J].药学服务与研究,2009,9(1):36-39.
[10]杨秀青,石征蓉,谷江华,等.银菊解毒口服液大孔树脂纯化工艺研究[J].中草药,2017,48(23):4904-4911.
[11]石振武,赵敏.运用层次分析法确定指标的权值[J].科技和产业,2008,8(2):23-25.
[12]刘小妹,施崇精,程中琴,等.人参水解物中人参二醇的工艺优化及其HPLC-ELSD含量测定方法[J/OL].天然产物研究与开发,2018-04-18.
[13]林丽,王琰,王福星,等.基于硫酸-蒽酮法对不同产地藏药线叶龙胆多糖的含量测定[J].中国中药杂志,2014,39(14):2774-2776.
[14]辛敏,刘轩,詹欣,等.硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较[J].中国实验方剂学杂志,2014,20(3):62-65.
[15]黄潇,刘婧,付小梅,等.基于CRITIC法计算权重系数的Box-Behnken响应面法优化栀子炭微波炮制工艺研究[J].中草药,2017,48(6):1133-1138.
[16]贾执瑛,谢燮,王晓艳,等.人参主要成分对大鼠免疫功能的比较研究[J].中国中药杂志,2014,39(17):3363-3366.
[17]郑智茵,尹利明,庄海峰,等.人参二醇组皂苷提取物对再生障碍性贫血小鼠免疫调节作用的研究[J].中国药理学通报,2015,31(6):790-795.