溶剂法提取松子壳总黄酮工艺的研究
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摘要
探究松子壳中总黄酮的最佳提取工艺条件.以松子壳粉为原材料,利用乙醇水浴加热提取,研究了提取时间、提取温度、乙醇体积分数、料液比对松子壳中总黄酮得率的影响,以总黄酮得率为响应值,进行响应面分析.结果表明松子壳的最佳提取工艺条件为:乙醇体积分数62%、料液比1︰28、提取温度60℃、提取时间1.2 h.此条件下松子壳中总黄酮的预测得率为5.878 mg/g,实际值为5.882 mg/g,实际值与预测值接近,说明响应面法分析结果较为合理、简单,可以得到松子壳提取总黄酮的最佳工艺条件.
Optimum extraction conditions of total flavonoids was studied from pine nut shell.The powder of pine nut shell was extracted with ethanol in a water bath. Extraction time,extraction temperature,ethanol concentration and ratio of solid to liquid were selected as influence factors,response surface analysis was carried out with the yield of total flavonoids as response value. The results showed that optimum conditions for total flavonoids were ethanol concentration 62%,solid-liquid ratio 1∶ 28,extraction temperature 60 ℃,extraction time1. 2 h. The predicted extraction yield of total flavonoids was 5. 878 mg/g,and the verification5. 882 mg/g. The actual value was close to the predicted value. The results showed that the response surface method was more reasonable and simple,and the optimum extraction conditions of total flavonoids from pine nut shell could be obtained.
Optimum extraction conditions of total flavonoids was studied from pine nut shell.The powder of pine nut shell was extracted with ethanol in a water bath. Extraction time,extraction temperature,ethanol concentration and ratio of solid to liquid were selected as influence factors,response surface analysis was carried out with the yield of total flavonoids as response value. The results showed that optimum conditions for total flavonoids were ethanol concentration 62%,solid-liquid ratio 1∶ 28,extraction temperature 60 ℃,extraction time1. 2 h. The predicted extraction yield of total flavonoids was 5. 878 mg/g,and the verification5. 882 mg/g. The actual value was close to the predicted value. The results showed that the response surface method was more reasonable and simple,and the optimum extraction conditions of total flavonoids from pine nut shell could be obtained.
引文
[1]任华东,段福文,王祖芳.世界松籽资源生产利用现状及我国松籽发展对策探讨[J].世界林业研究,2006,19(2):28-33.
[2]张大伟,郝琳琳,李明谦,等.红松松籽壳和松塔天然产物研究进展[J].时珍国医国药,2012,23(10):2588-2590.
[3]丁宁.红松松子壳多糖的提取纯化、组成分析及抗氧化活性研究[D].青岛:青岛科技大学,2014.
[4]李明谦.红松松子壳多糖超声提取工艺的建立与抗肿瘤活性的研究[D].长春:吉林大学,2013.
[5]付昊雨.红松松子壳多糖的分离纯化、结构表征及其抗氧化活性研究[D].长春:吉林大学,2017.
[6]MICHIHARA S,ANAKA T,ZAWA Y,et al.Puerarin exerted anti-osteoporotic action independent of estrogen receptor-mediatedpathway[J].J Nutr Sci Vitaminol(Tokyo),2012,8(3):202-209.
[7]王慧.黄酮类化合物生物活性的研究进展[J].食品与药品,2012,12(9):347-350.
[8]何佳珂,于洋,陈西敬,等.黄酮类化合物的药物代谢研究进展[J].中国中药杂志,2010,35(21):2789-2794.
[9]朱文振,马龙,李国荣.黄酮类化合物的抗癌作用及作用机制[J].生命科学,2012,4(5):444-449.
[10]BATRA P,SHARMA A K.Anti-cancer potential of flavonoids:recent trends and future perspectives[J].Biotech,2013,3(6):439-459.
[11]张黎明,李瑞超,郝利民,等.响应面优化玛咖叶总黄酮提取工艺及其抗氧化活性研究[J].现代食品科技,2014,30(4):233-239.
[12]黎晨晨,张煜,迟逍遥,等.响应面法优化花椰菜总黄酮提取工艺的研究[J].河南工业大学学报:自然科学版,2018,39(2):87-92.
[13]孙雪婷,蒋玉蓉,袁俊杰,等.响应面法优化提取藜麦种子黄酮及抗氧化活性[J].中国食品学报,2017,17(03):127-135.
[14]李珍,哈益明,李安,等.响应面优化苹果皮渣多酚超声提取工艺研究[J].中国农业科学,2013,46(21):4569-4577.
[15]吴子江.无花果叶类黄酮提取、纯化、鉴定及抗氧化研究[D].福州:福建农林大学,2013.
[2]张大伟,郝琳琳,李明谦,等.红松松籽壳和松塔天然产物研究进展[J].时珍国医国药,2012,23(10):2588-2590.
[3]丁宁.红松松子壳多糖的提取纯化、组成分析及抗氧化活性研究[D].青岛:青岛科技大学,2014.
[4]李明谦.红松松子壳多糖超声提取工艺的建立与抗肿瘤活性的研究[D].长春:吉林大学,2013.
[5]付昊雨.红松松子壳多糖的分离纯化、结构表征及其抗氧化活性研究[D].长春:吉林大学,2017.
[6]MICHIHARA S,ANAKA T,ZAWA Y,et al.Puerarin exerted anti-osteoporotic action independent of estrogen receptor-mediatedpathway[J].J Nutr Sci Vitaminol(Tokyo),2012,8(3):202-209.
[7]王慧.黄酮类化合物生物活性的研究进展[J].食品与药品,2012,12(9):347-350.
[8]何佳珂,于洋,陈西敬,等.黄酮类化合物的药物代谢研究进展[J].中国中药杂志,2010,35(21):2789-2794.
[9]朱文振,马龙,李国荣.黄酮类化合物的抗癌作用及作用机制[J].生命科学,2012,4(5):444-449.
[10]BATRA P,SHARMA A K.Anti-cancer potential of flavonoids:recent trends and future perspectives[J].Biotech,2013,3(6):439-459.
[11]张黎明,李瑞超,郝利民,等.响应面优化玛咖叶总黄酮提取工艺及其抗氧化活性研究[J].现代食品科技,2014,30(4):233-239.
[12]黎晨晨,张煜,迟逍遥,等.响应面法优化花椰菜总黄酮提取工艺的研究[J].河南工业大学学报:自然科学版,2018,39(2):87-92.
[13]孙雪婷,蒋玉蓉,袁俊杰,等.响应面法优化提取藜麦种子黄酮及抗氧化活性[J].中国食品学报,2017,17(03):127-135.
[14]李珍,哈益明,李安,等.响应面优化苹果皮渣多酚超声提取工艺研究[J].中国农业科学,2013,46(21):4569-4577.
[15]吴子江.无花果叶类黄酮提取、纯化、鉴定及抗氧化研究[D].福州:福建农林大学,2013.