响应面法优选复方红参丹富氧分散片的最佳提取工艺及活性研究
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摘要
目的:采用响应面法研究复方红参丹富氧分散片的最佳提取工艺及其提高机体缺氧耐受力的活性研究。方法:以红景天苷、丹参酮ⅡA、丹酚酸B、总多糖、总皂苷提取率所得综合评分为考察指标,利用响应面法对提取工艺中的提取时间、提取次数、溶媒量进行进一步的参数优选研究,从而优选出复方红参丹富氧分散片的最佳提取工艺;采用常压耐缺氧、急性脑缺氧、心肌细胞缺氧耐受力实验方法对复方红参丹富氧分散片提取物进行活性研究。结果:复方红参丹富氧分散片最优提取工艺为溶媒量8倍,提取3次,每次2.5h;复方红参丹富氧分散片提取物明显提高机体的缺氧耐受力。结论:响应面法优选工艺简便高效,为确保开发出安全有效、适合工业化生产的复方红参丹富氧分散片提供了实验依据与技术支撑。
Objective: To study on the optimal extraction technology of Compound Hongshendan Oxygen Rich Dispersible Tablets by response surface methodology and its effect of improving the activity of hypoxia tolerance of the body.Methods: Taking the comprehensive scores of salidroside, tanshinone ⅡA, salvianolic acid B, total polysaccharides and total saponins as the evaluation indexes, the response surface methodology was used to further optimize the extraction time, extraction times and solvent content in the extraction process, so as to optimize the extraction process of Compound Hongshendan OxygenEnriched Dispersible Tablets. The activity of Compound Hongshendan Oxygen Rich Dispersible Tablets was studied by normobaric hypoxia tolerance, acute cerebral hypoxia, and myocardial cell hypoxia tolerance test method. Results: The parameter of the optimal extraction process of the Compound Hongshendan Oxygen Rich Dispersible Tablets was as follows: 8 times water for extracting for 3 times and 2.5 hours each time. The extracts of Compound Hongshendan Oxygen Rich Dispersible Tablets could obviously improve the hypoxia tolerance of the body. Conclusion: The response surface methodology is simple and effective optimal process, which provides a experimental basis and technical support to ensure the development of Compound Hongshendan Oxygen Rich Dispersible Tablets is safe, effective and suitable for industrial production.
Objective: To study on the optimal extraction technology of Compound Hongshendan Oxygen Rich Dispersible Tablets by response surface methodology and its effect of improving the activity of hypoxia tolerance of the body.Methods: Taking the comprehensive scores of salidroside, tanshinone ⅡA, salvianolic acid B, total polysaccharides and total saponins as the evaluation indexes, the response surface methodology was used to further optimize the extraction time, extraction times and solvent content in the extraction process, so as to optimize the extraction process of Compound Hongshendan OxygenEnriched Dispersible Tablets. The activity of Compound Hongshendan Oxygen Rich Dispersible Tablets was studied by normobaric hypoxia tolerance, acute cerebral hypoxia, and myocardial cell hypoxia tolerance test method. Results: The parameter of the optimal extraction process of the Compound Hongshendan Oxygen Rich Dispersible Tablets was as follows: 8 times water for extracting for 3 times and 2.5 hours each time. The extracts of Compound Hongshendan Oxygen Rich Dispersible Tablets could obviously improve the hypoxia tolerance of the body. Conclusion: The response surface methodology is simple and effective optimal process, which provides a experimental basis and technical support to ensure the development of Compound Hongshendan Oxygen Rich Dispersible Tablets is safe, effective and suitable for industrial production.
引文
[1]Kelly G S.Rhodiola rosea:A possible plant adeptogen.Altema Med Rev,2001,6(3):293-302
[2]金雪莲.红景天苷在不同缺氧状况下抗缺氧作用的实验研究.卫生职业教育,2012,30(3):121-122
[3]张民,张骅,徐鹏.丹参酮ⅡA的药理作用研究进展.医药导报,2008,27(10):1237-1239
[4]蔡丽萍,习志刚,杨红.丹参酮的药理作用和临床研究进展.广东药学院学报,2008,24(3):321-324
[5]吴杲,何招兵,吴汉斌.丹参酮的药理作用研究进展.现代中西医结合杂志,2005,14(10):1382-1385
[6]尹东锋,陈效.丹参水提取物对小鼠耐缺氧作用的影响.实验研究,2012,9(6):33-35
[7]汤佩莲,何飞武,潘甜美.丹参水溶性成分对中枢神经系统缺血缺氧的保护.河北医学,2006,6(12):523-525
[8]孔宏亮,侯爱洁,陈晓明,等.HIF-1α介导人参皂甙Rb1对缺氧心肌细胞葡萄糖代谢的调节.中国病理生理杂志,2016,32(9):1621-1626
[9]安明,赵国君,韦新成.人参皂苷Rg1保护心血管系统和中枢神经系统的药理活性进展.中国临床药理学杂志,2012,28(1):75-77
[10]Beall C M.Tibetan and andean patterns of adaptation to highaltitude hypoxia.Hum Biol,2000,72(1):201-228
[11]Nieber K,Eschke D,Brand A.Brain hypoxia:Effects of ATP and adenosine.Prog Brain Res,1999,83(1):21-37
[12]King H S,Zhang Y H,Fang L H,et al.Effects of ginsenosides on bovine adrenal tyrosine hydroxylase.J Ethnopharmacol,1999,66(1):107-111
[13]陈立波,赵洪序,宋翔翔,等.人参皂甙对兔心肌缺血再灌注损伤的保护作用及其浓度-效应关系.白求恩医科大学学报,1994,20:442-443
[14]王浴生.中药药理与应用.北京:人民卫生出版社,1983:23-39
[15]李磊,戴金凤,王小如,等.丹参提取物耐缺氧功能及营养化学研究.食品科学,2005,4(26):232-235
[16]龙怡,李佳川,孟宪丽.红景天有效成分对缺氧缺糖心肌细胞损伤的保护作用研究.中药药理与临床,2010,26(1):24-25
[17]徐瑶瑶.复合人参素及其所含人参多糖的药理作用对比研究.海峡药学,2015,27(12):28-29
[18]国家药典委员会.中华人民共和国药典.北京:中国医药科技出版社,2015
[19]张小红,梁洁,肖雪,等.HPLC法测定林芝地区丹参中丹参酮Ⅱa含量研究.亚太传统医药,2015,11(12):17-19
[20]靳维荣,孙强,单成钢,等.丹参药材中丹参酮ⅡA含量测定方法改进.时珍国医国药,2010,21(7):1672-1673
[21]薛静,叶正良,李德坤,等.HPLC同时测定丹参多酚酸中丹酚酸B,迷迭香酸,紫草酸,丹酚酸B的含量.中国实验方剂学杂志,2013,19(3):70-72
[22]丁菊英,张典瑞.芪参玉液胶囊中丹酚酸B的含量测定.中国实验方剂学杂志,2010,16(1):35-36
[23]陈旭,党晓芳,曹飒丽,等.三七中总皂苷含量测定的对照品筛选.中国实验方剂学杂志,2013,19(7):66-68
[24]刘晓棠,张卫明,姜洪芳,等.不同种类木瓜样品的总皂苷含量测定.食品工业科技,2009,30(4):324-326
[25]黄樱华,李赐恩,萧月兴.人参叶中总皂苷及人参皂苷Rg1、Re含量测定.亚太传统医药,2013,9(5):49-51
[26]钟岩,潘浦群,王艳红,等.苯酚-硫酸法测定鲜人参中多糖含量.时珍国医国药,2008,19(8):1957-1958
[27]李计萍.中药新药研究中多糖含量测定方法探讨.中国中药杂志,2014,39(17):3392-3394
[28]Gupta S,Wen J J,Garg N J.Oxidative stress in chagas disease.Inerdiscip Perspect Infect Dis,2009,2009:190354
[29]Lakshmi S V,Padmaja G,Kuppusamy P,et al.Oxidative stress in cardiovascular disease.Indian J Biochem Biophys,2009,46(6):421-440
[30]孙琳,柴智,张涛.木豆叶提取物对H2O2诱导的H9c2细胞氧化损伤的保护作用及机制研究.中草药,2016,47(2):297-300
[31]张双伟,李润美,徐进文,等.毛冬青皂苷E对H9C2心肌细胞缺氧/复氧损伤的影响.中药新药与临床药理,2015,26(5):591-595
[2]金雪莲.红景天苷在不同缺氧状况下抗缺氧作用的实验研究.卫生职业教育,2012,30(3):121-122
[3]张民,张骅,徐鹏.丹参酮ⅡA的药理作用研究进展.医药导报,2008,27(10):1237-1239
[4]蔡丽萍,习志刚,杨红.丹参酮的药理作用和临床研究进展.广东药学院学报,2008,24(3):321-324
[5]吴杲,何招兵,吴汉斌.丹参酮的药理作用研究进展.现代中西医结合杂志,2005,14(10):1382-1385
[6]尹东锋,陈效.丹参水提取物对小鼠耐缺氧作用的影响.实验研究,2012,9(6):33-35
[7]汤佩莲,何飞武,潘甜美.丹参水溶性成分对中枢神经系统缺血缺氧的保护.河北医学,2006,6(12):523-525
[8]孔宏亮,侯爱洁,陈晓明,等.HIF-1α介导人参皂甙Rb1对缺氧心肌细胞葡萄糖代谢的调节.中国病理生理杂志,2016,32(9):1621-1626
[9]安明,赵国君,韦新成.人参皂苷Rg1保护心血管系统和中枢神经系统的药理活性进展.中国临床药理学杂志,2012,28(1):75-77
[10]Beall C M.Tibetan and andean patterns of adaptation to highaltitude hypoxia.Hum Biol,2000,72(1):201-228
[11]Nieber K,Eschke D,Brand A.Brain hypoxia:Effects of ATP and adenosine.Prog Brain Res,1999,83(1):21-37
[12]King H S,Zhang Y H,Fang L H,et al.Effects of ginsenosides on bovine adrenal tyrosine hydroxylase.J Ethnopharmacol,1999,66(1):107-111
[13]陈立波,赵洪序,宋翔翔,等.人参皂甙对兔心肌缺血再灌注损伤的保护作用及其浓度-效应关系.白求恩医科大学学报,1994,20:442-443
[14]王浴生.中药药理与应用.北京:人民卫生出版社,1983:23-39
[15]李磊,戴金凤,王小如,等.丹参提取物耐缺氧功能及营养化学研究.食品科学,2005,4(26):232-235
[16]龙怡,李佳川,孟宪丽.红景天有效成分对缺氧缺糖心肌细胞损伤的保护作用研究.中药药理与临床,2010,26(1):24-25
[17]徐瑶瑶.复合人参素及其所含人参多糖的药理作用对比研究.海峡药学,2015,27(12):28-29
[18]国家药典委员会.中华人民共和国药典.北京:中国医药科技出版社,2015
[19]张小红,梁洁,肖雪,等.HPLC法测定林芝地区丹参中丹参酮Ⅱa含量研究.亚太传统医药,2015,11(12):17-19
[20]靳维荣,孙强,单成钢,等.丹参药材中丹参酮ⅡA含量测定方法改进.时珍国医国药,2010,21(7):1672-1673
[21]薛静,叶正良,李德坤,等.HPLC同时测定丹参多酚酸中丹酚酸B,迷迭香酸,紫草酸,丹酚酸B的含量.中国实验方剂学杂志,2013,19(3):70-72
[22]丁菊英,张典瑞.芪参玉液胶囊中丹酚酸B的含量测定.中国实验方剂学杂志,2010,16(1):35-36
[23]陈旭,党晓芳,曹飒丽,等.三七中总皂苷含量测定的对照品筛选.中国实验方剂学杂志,2013,19(7):66-68
[24]刘晓棠,张卫明,姜洪芳,等.不同种类木瓜样品的总皂苷含量测定.食品工业科技,2009,30(4):324-326
[25]黄樱华,李赐恩,萧月兴.人参叶中总皂苷及人参皂苷Rg1、Re含量测定.亚太传统医药,2013,9(5):49-51
[26]钟岩,潘浦群,王艳红,等.苯酚-硫酸法测定鲜人参中多糖含量.时珍国医国药,2008,19(8):1957-1958
[27]李计萍.中药新药研究中多糖含量测定方法探讨.中国中药杂志,2014,39(17):3392-3394
[28]Gupta S,Wen J J,Garg N J.Oxidative stress in chagas disease.Inerdiscip Perspect Infect Dis,2009,2009:190354
[29]Lakshmi S V,Padmaja G,Kuppusamy P,et al.Oxidative stress in cardiovascular disease.Indian J Biochem Biophys,2009,46(6):421-440
[30]孙琳,柴智,张涛.木豆叶提取物对H2O2诱导的H9c2细胞氧化损伤的保护作用及机制研究.中草药,2016,47(2):297-300
[31]张双伟,李润美,徐进文,等.毛冬青皂苷E对H9C2心肌细胞缺氧/复氧损伤的影响.中药新药与临床药理,2015,26(5):591-595