分子蒸馏法富集茶树油特征成分的研究
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摘要
以茶树油中1,8-桉叶素和松油烯-4-醇的相对含量为指标,借助刮膜式分子蒸馏技术,在单因素试验基础上进行正交试验,并通过气相色谱法测定相对含量,得出在蒸馏温度38℃,蒸馏压力110 Pa,进料速率2.5 mL/min,刮膜转速450 r/min条件下,轻组分中1,8-桉叶素相对含量由2.674%提升至5.33%;在蒸馏温度38℃,蒸馏压力110 Pa,进料速率3 mL/min,刮膜转速450 r/min,重组分中松油烯-4-醇的相对含量由43.416%提升至65.17%。运用该技术,富集效果明显,能为挥发油类物质的工业化生产提供参考价值。
With the relative content of 1,8-cineole and terpinene-4-ol in tea tree oil as the index,the orthogonal experiment was carried out on the basis of single factor experiment by means of wiped-film molecular distillation technology,and the relative content was determined by gas chromatography. The results showed that the distillation temperature was 38 ℃,the distillation pressure was 110 Pa,the feed rate was 2.5 mL/min,and the scraping film was rotated. At 450 r/min,the relative content of 1,8-cineole in the light component increased from 2.674 % to 5.33 %. At 38 ℃,110 Pa,3 mL/min feed rate,450 r/min scraping speed,the relative content of terpinene-4-ol in the reconstituted component increased from 43.416 % to 65.17 %. Using this technology,the enrichment effect is obvious,and it can provide reference value for industrialized production of volatile oils.
With the relative content of 1,8-cineole and terpinene-4-ol in tea tree oil as the index,the orthogonal experiment was carried out on the basis of single factor experiment by means of wiped-film molecular distillation technology,and the relative content was determined by gas chromatography. The results showed that the distillation temperature was 38 ℃,the distillation pressure was 110 Pa,the feed rate was 2.5 mL/min,and the scraping film was rotated. At 450 r/min,the relative content of 1,8-cineole in the light component increased from 2.674 % to 5.33 %. At 38 ℃,110 Pa,3 mL/min feed rate,450 r/min scraping speed,the relative content of terpinene-4-ol in the reconstituted component increased from 43.416 % to 65.17 %. Using this technology,the enrichment effect is obvious,and it can provide reference value for industrialized production of volatile oils.
引文
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[9]胡雪芳,田志清,裴海生,等.短程分子蒸馏技术精制巨尾桉叶精油工艺优化[J].农业工程学报,2018,34(2):299-307
[10]刘克海,陈秋林,谢晶,等.分子蒸馏法富集甜橙油特征香气成分[J].食品科学,2012,33(10):200-203
[11]罗盛旭,梁振益,杨雪蕊.气相色谱-质谱联用分析茶树油的成分[J].化学分析计量,2003,12(5):37-38
[2]王懿,王振维.茶树油灭活脊髓灰质炎病毒的试验观察[J].第三军医大学学报,2004,26(13):1169-1170
[3]秦文文,梁剑平,郝宝成,等.茶树油对5种常见致病菌的体外抑菌作用研究[J].黑龙江畜牧兽医,2017(21):195-199
[4]文洁,李婕,曹维.天然茶树油消毒剂抗菌活性与应用前景[J].中药材,2011,34(3):487-489
[5]黄德浩,吴伟超,吴国兴,等.茶树精油抗炎抗菌功效的研究现状[J].今日药学,2018(3):211-215
[6]尹伦甫.水产用抗寄生虫药研发新动态——植物精油杀虫活性因子的应用[J].北京水产,2007(6):13-14
[7]Riccioni L,Orzali L.Activity of tea tree(Melaleuca alternifolia,Cheel)andthyme(Thymus vulgaris,Linnaeus.)essential oils against some PAthogenic seed borne fungi[J].Journal of Essential Oil Research,2011,23(6):43-47
[8]吴頔,谢吉蓉,宋琴,等.茶树油作为天然抗菌剂的研究进展[J].中国药学杂志,2013,48(21):1803-1807
[9]胡雪芳,田志清,裴海生,等.短程分子蒸馏技术精制巨尾桉叶精油工艺优化[J].农业工程学报,2018,34(2):299-307
[10]刘克海,陈秋林,谢晶,等.分子蒸馏法富集甜橙油特征香气成分[J].食品科学,2012,33(10):200-203
[11]罗盛旭,梁振益,杨雪蕊.气相色谱-质谱联用分析茶树油的成分[J].化学分析计量,2003,12(5):37-38