新型复合脉冲不可逆电穿孔治疗肿瘤关键技术及临床应用研究进展
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摘要
癌症是威胁人类健康的主要杀手之一。虽然传统微秒脉冲不可逆电穿孔治疗肿瘤方法以其非热效应的优势,在临床实体瘤治疗中得到了大量的应用并取得了良好的治疗效果,但是随着临床研究的深入,发现在治疗过程中仍存在电场分布不均致肿瘤残留以及肌肉收缩等亟需解决的问题。临床需求催生并促进了新一代复合脉冲不可逆电穿孔治疗肿瘤方法的发展与成熟。在简要叙述传统不可逆电穿孔发展历程的基础上,指出目前不可逆电穿孔临床应用存在的相关问题,并针对这些问题引出复合脉冲不可逆电穿孔的肿瘤新型治疗方法;重点介绍了复合脉冲不可逆电穿孔的发展历程及研究成果;以及由我国研究人员独立研发并成功开展的新型复合脉冲不可逆电穿孔治疗前列腺癌的世界首批临床实验,验证了复合脉冲临床应用的安全性与有效性。最后指出了不可逆电穿孔疗法的应用前景和发展趋势。
Tumor becomes one of the major killers to human health.Although the conventional microsecond pulse irreversible electroporation has been widely applied in solid tumor therapy and achieved great effects because of the non-thermal ablation,some problems still need to be solved with the clinical process,such as incomplete ablation due to the non-uniform distribution of electric field and muscle contraction during treatment.The clinical demands give rise to a new generation irreversible electroporation—composite pulse irreversible electroporation.In this paper,we firstly introduced the development of the traditional irreversible electroporation and its problems,then the appearance of composite pulse,especially,the development and the achievement of composite pulse were described.Secondly,the design of the composite pulse apparatus and the first clinical trial on human prostate in the world by Chinese researchers were mentioned,which verified the safety and efficiency of composite pulse in tumor ablation.Finally,we pointed out the application prospect and development tendency of composite pulse irreversible electroporation.
Tumor becomes one of the major killers to human health.Although the conventional microsecond pulse irreversible electroporation has been widely applied in solid tumor therapy and achieved great effects because of the non-thermal ablation,some problems still need to be solved with the clinical process,such as incomplete ablation due to the non-uniform distribution of electric field and muscle contraction during treatment.The clinical demands give rise to a new generation irreversible electroporation—composite pulse irreversible electroporation.In this paper,we firstly introduced the development of the traditional irreversible electroporation and its problems,then the appearance of composite pulse,especially,the development and the achievement of composite pulse were described.Secondly,the design of the composite pulse apparatus and the first clinical trial on human prostate in the world by Chinese researchers were mentioned,which verified the safety and efficiency of composite pulse in tumor ablation.Finally,we pointed out the application prospect and development tendency of composite pulse irreversible electroporation.
引文
[1]CHEN W,ZHENG R,BAADE P D,et al.Cancer statistics in China,2015[J].A Cancer Journal for Clinicians,2016,66(2):115-132.
[2]李莹,邹鹏飞,刘兴发,等.极低频磁场的生物学效应[J].高电压技术,2017,43(2):567-577.LI Ying,ZOU Pengfei,LIU Xingfa,et al.Biological effects of extremely low frequency magnetic fields[J].High Voltage Engineering,2017,43(2):567-577.
[3]李成祥,张睿哲,姚陈果,等.高强度皮秒脉冲电场抑制HUVEC迁移、增殖及小管形成的实验研究[J].高电压技术,2017,43(2):587-593.LI Chengxiang,ZHANG Ruizhe,YAO Chenguo,et al.Experimental study on inhibition effect of HUVEC migration,proliferation and tube formation by intense picosecond pulsed electric field[J].High Voltage Engineering,2017,43(2):587-593.
[4]WEAVER J C,CHIZMADZHEV Y A.Theory of electroporation:a review[J].Bioelectrochemistry and Bioenergetics,1996,41(2):135-160.
[5]KOTNIK T,KRAMAR P,PUCIHAR G,et al.Cell membrane electroporation-part 1:the phenomenon[J].IEEE Electrical Insulation Magazine,2012,28(5):14-23.
[6]HABERL S,MIKLAV?I?D,SER?A G,et al.Cell membrane electroporation-part 2:the applications[J].IEEE Electrical Insulation Magazine,2013,29(1):29-37.
[7]YARMUSH M L,GOLBERG A,SER?A G,et al.Electroporation-based technologies for medicine:principles,applications,and challenges[J].Annual Review of Biomedical Engineering,2014,16(16):295-320.
[8]赵亚军,姚陈果,董守龙,等.基于遗传算法的不可逆电穿孔肿瘤消融治疗计划的优化[J].高电压技术,2017,43(2):627-636.ZHAO Yajun,YAO Chenguo,DONG Shoulong,et al.Optimization of the treatment planning for the tumor ablation of irreversible electroporation based on genetic algorithm[J].High Voltage Engineering,2017,43(2):627-636.
[9]姚陈果,赵亚军,李成祥,等.不可逆电穿孔微创消融肿瘤技术的研究进展[J].高电压技术,2014,40(12):3725-3737.YAO Chenguo,ZHAO Yajun,LI Chengxiang,et al.Recent advances in tissue minimally invasive ablation with irreversible electroporation[J].High Voltage Engineering,2014,40(12):3725-3737.
[10]李成祥,姚陈果,米彦,等.物理消融肿瘤的研究进展[J].生物医学工程学杂志,2009(5):1137-1140.LI Chengxiang,YAO Chenguo,MI Yan,et al.Recent advances on physical ablation for tumor[J].Journal of Biomedical Engineering,2009(5):1137-1140.
[11]LENCIONI R,CROCETTI L.Radio frequency ablation of liver cancer[J].Techniques in Vascular and Interventional Radiology,2007,10(1):38-46.
[12]于哲,吴家昌,张明华,等.微波消融治疗骨肉瘤诱导免疫原性细胞死亡的机制研究[J].中华放射肿瘤学杂志,2016,25(6):602-608.YU Zhe,WU Jiachang,ZHANG Minghua,et al.Mechanism of immunogenic cell death induced by microwave ablation treatment of osteosarcoma[J].Chinese Journal of Radiation Oncology,2016,25(6):602-608.
[13]何志祝,郑永昌,刘静.脑肿瘤冷冻消融区域影响因素分析[J].工程热物理学报,2016,37(8):1753-1757.HE Zhizhu,ZHENG Yongchang,LIU Jing.Analyzing the influence factors of brain tumor cryoablation[J].Journal of Engineering Thermophysics,2016,37(8):1753-1757.
[14]YAO C G,SUN C X,MI Y,et al.Experimental studies on killing and inhibiting effects of steep pulsed electric field(SPEF)to target cancer cell and solid tumor[J].IEEE Transactions on Plasma Science,2004,32(4):1626-1633.
[15]TANG L L,YAO C G,SUN C X.Apoptosis induction with electric pulses—a new approach to cancer therapy with drug free[J].Biochemical and Biophysical Research Communications,2009,390(4):1098-1101.
[16]YAO C G,HU X,MI Y,et al.Window effect of pulsed electric field on biological cells[J].IEEE Transactions on Dielectrics and Electrical Insulation,2009,16(5):1259-1266.
[17]LIU Y,XIONG Z A,ZHOU W,et al.Percutaneous ultrasound-guided irreversible electroporation:a goat liver study[J].Oncology Letters,2012,4(3):450-454.
[18]DAVALOS R V,MIR L M,RUBINSKY B.Tissue ablation with irreversible electroporation[J].Annals of Biomedical Engineering,2005,33(2):223-231.
[19]MILLER L,LEOR J,RUBINSKY B.Cancer cells ablation with irreversible electroporation[J].Technology in Cancer Research&Treatment,2005,4(6):699-705.
[20]GARCIA P A,ROSSMEISL J H,II R E N,et al.Intracranial nonthermal irreversible electroporation:in vivo analysis[J].Journal of Membrane Biology,2010,236(1):127-136.
[21]GARCIA P A,DAVALOS R V,MIKLAVCIC D.A numerical investigation of the electric and thermal cell kill distributions in electroporation-based therapies in tissue[J].Plos One,2014,9(8):e103083.
[22]NEAL R E,GARCIA P A,ROSSMEISL J H,et al.A study using irreversible electroporation to treat large,irregular tumors in a canine patient[C]∥International Conference of the IEEE Engineering in Medicine&Biology Society.[S.l.]:IEEE,2010:2747.
[23]ONIK G,RUBINSKY B.Irreversible electroporation:first patient experience focal therapy of prostate cancer[M].Berlin,Germany:Springer,2010:235-247.
[24]GUO Y,ZHANG Y,KLEIN R,et al.Irreversible electroporation therapy in the liver:longitudinal efficacy studies in a rat model of hepatocellular carcinoma[J].Cancer Research,2010,70(4):1555-1563.
[25]QIN Z,JIANG J,LONG G,et al.Irreversible electroporation:an in vivo study with dorsal skin fold chamber[J].Annals of Biomedical Engineering,2013,41(3):619-629.
[26]PHILIPS P,LI Y,LI S,et al.Efficacy of irreversible electroporation in human pancreatic adenocarcinoma:advanced murine model[J].Molecular Therapy—Methods&Clinical Development,2015,2:15001.
[27]THOMSON K R,CHEUNG W,ELLIS S J,et al.Investigation of the safety of irreversible electroporation in humans[J].Journal of Vascular&Interventional Radiology Jvir,2011,22(5):611-621.
[28]KINGHAM T P,KARKAR A M,D'ANGELICA M I,et al.Ablation of perivascular hepatic malignant tumors with irreversible electroporation[J].Journal of the American College of Surgeons,2012,215(3):379-387.
[29]万军平.肝动脉介入热化疗治疗转移性肝肿瘤的临床应用[J].临床医学工程,2012,19(11):1917-1918.WANG Junping.Clinical application of metastatic liver treated by hepatic artery involved thermo-chemotherapy[J].Medicine Healthcare Apparatus,2012,19(11):1917-1918.
[30]谢晓燕,吕明德,殷晓煜,等.超声引导经皮射频消融治疗肝癌的研究[J].中华外科杂志,2003,41(1):23-26.XIE Xiaoyan,LüMingde,YIN Xiaoyu,et al.Application of ultrasound-guided percutaneous radiofrequency ablation in treatment of liver cancer[J].Chinese Journal of Surgery,2003,41(1):23-26.
[31]CHEUNG W,KAVNOUDIAS H,ROBERTS S,et al.Irreversible electroporation for unresectable hepatocellular carcinoma:initial experience and review of safety and outcomes[J].Technology in Cancer Research&Treatment,2013,12(3):233-241.
[32]GOLBERG A,YARMUSH M L.Nonthermal irreversible electroporation:fundamentals,applications,and challenges[J].IEEE Transactions on Biomedical Engineering,2013,60(3):707-714.
[33]MARTIN R C,SCHWARTZ E,ADAMS J,et al.Intra-operative anesthesia management in patients undergoing surgical irreversible electroporation of the pancreas,liver,kidney,and retroperitoneal tumors[J].Anesthesiology and Pain Medicine,2015,5(3):e22786.
[34]GOLBERG A,BRUINSMA B G,UYGUN B E,et al.Tissue heterogeneity in structure and conductivity contribute to cell survival during irreversible electroporation ablation by“electric field sinks”[J].Scientific Reports,2015,5:8485.
[35]董守龙.高频双极性微秒脉冲电场不可逆电穿孔消融肿瘤的实验与机理研究[D].重庆:重庆大学,2017.DONG Shoulong.Experiments and mechanism research of irreversible electroporation by high frequency bipolar microsecond pulse for tumor ablation[D].Chongqing,China:Chongqing University,2017.
[36]ARENA C B,SANO M B,RYLANDER M N,et al.Theoretical considerations of tissue electroporation with high-frequency bipolar pulses[J].IEEE Transactions on Biomedical Engineering,2011,58(5):1474-1482.
[37]MIKLAV?I?D,PUCIHAR G,PAVLOVEC M,et al.The effect of high frequency electric pulses on muscle contractions and antitumor efficiency in vivo for a potential use in clinical electrochemotherapy[J].Bioelectrochemistry,2005,65:121-128.
[38]MI Y,RUI S Q,LI C X,et al.Multi-parametric study of temperature and thermal damage of tumor exposed to high-frequency nanosecond-pulsed electric fields based on finite element simulation[J].Medical&Biological Engineering&Computing,2017,55(7):1109-1122.
[39]ARENA C B,SANO M B,ROSSMEISL J H,et al.High-frequency irreversible electroporation(H-FIRE)for non-thermal ablation without muscle contraction[J].Biomedical Engineering Online,2011,10(1):102.
[40]SWEENEY D C,REBER?EK M,DERMOL J,et al.Quantification of cell membrane permeability induced by monopolar and high-frequency bipolar bursts of electrical pulses[J].Biochimica et Biophysica Acta(BBA)-Biomembranes,2016,1858(11):2689-2698.
[41]IVEY J W,LATOUCHE E L,SANO M B,et al.Targeted cellular ablation based on the morphology of malignant cells[J].Scientific Reports,2015,5:17157.
[42]YAO C G,LIU H M,ZHAO Y J,et al.Analysis of dynamic processes in single-cell electroporation and their effects on parameter selection based on the finite-element model[J].IEEE Transactions on Plasma Science,2017,45(5):889-900.
[43]YAO C,ZHAO Y,DONG S,et al.Differences in the effects of duty cycle and interval on cell response induced by high-frequency pulses under different pulse durations[J].IEEE Transactions on Plasma Science,2016,44(10):2097-2110.
[44]SANO M B,ARENA C B,DEWITT M R,et al.In-vitro bipolar nano-and microsecond electro-pulse bursts for irreversible electroporation therapies[J].Bioelectrochemistry,2014,100:69-79.
[45]YAO C G,ZHAO Y J,MI Y,et al.Comparative study of the biological responses to conventional pulse and high-frequency monopolar pulse bursts[J].IEEE Transactions on Plasma Science,2017,45(10):2629-2638.
[46]YAO C G,ZHAO Y J,LIU H M,et al.Dielectric variations of potato induced by irreversible electroporation under different pulses based on the cole-cole model[J].IEEE Transactions on Dielectrics and Electrical Insulation,2017,24(4):2225-2233.
[47]BHONSLE S P,ARENA C B,SWEENEY D C,et al.Mitigation of impedance changes due to electroporation therapy using bursts of high-frequency bipolar pulses[J].Biomedical Engineering Online,2015,14(3):1-14.
[48]SANO M B,ARENA C B,BITTLEMAN K R,et al.Bursts of bipolar microsecond pulses inhibit tumor growth[J].Scientific Reports,2015,5:14999.
[49]ARENA C B,GARCIA P A,SANO M B,et al.Focal blood-brainbarrier disruption with high-frequency pulsed electric fields[J].Technology,2014,2(3):206-213.
[50]YAO C G,DONG S L,ZHAO Y J,et al.Bipolar microsecond pulses and insulated needle electrodes for reducing muscle contractions during irreversible electroporation[J].IEEE Transactions on Biomedical Engineering,2017,64(12):2924-2937.
[51]姚陈果,赵亚军,董守龙,等.基于阻抗谱的脉冲电场诱导兔肝脏组织不可逆电穿孔程度评估新方法的实验研究[J].高电压技术,2017,43(8):2454-2463.YAO Chenguo,ZHAO Yajun,DONG Shoulong,et al.Study on the impedance characteristic of rabbit liver induced by irreversible electroporation caused by high-frequency and conventional pulsed electric field[J].High Voltage Engineering,2017,43(8):2454-2463.
[52]SIDDIQUI I A,LATOUCHE E L,DEWITT M R,et al.Induction of rapid,reproducible hepatic ablations using next-generation,high frequency irreversible electroporation(H-FIRE)in vivo[J].HPB,2016,18(9):726-734.
[2]李莹,邹鹏飞,刘兴发,等.极低频磁场的生物学效应[J].高电压技术,2017,43(2):567-577.LI Ying,ZOU Pengfei,LIU Xingfa,et al.Biological effects of extremely low frequency magnetic fields[J].High Voltage Engineering,2017,43(2):567-577.
[3]李成祥,张睿哲,姚陈果,等.高强度皮秒脉冲电场抑制HUVEC迁移、增殖及小管形成的实验研究[J].高电压技术,2017,43(2):587-593.LI Chengxiang,ZHANG Ruizhe,YAO Chenguo,et al.Experimental study on inhibition effect of HUVEC migration,proliferation and tube formation by intense picosecond pulsed electric field[J].High Voltage Engineering,2017,43(2):587-593.
[4]WEAVER J C,CHIZMADZHEV Y A.Theory of electroporation:a review[J].Bioelectrochemistry and Bioenergetics,1996,41(2):135-160.
[5]KOTNIK T,KRAMAR P,PUCIHAR G,et al.Cell membrane electroporation-part 1:the phenomenon[J].IEEE Electrical Insulation Magazine,2012,28(5):14-23.
[6]HABERL S,MIKLAV?I?D,SER?A G,et al.Cell membrane electroporation-part 2:the applications[J].IEEE Electrical Insulation Magazine,2013,29(1):29-37.
[7]YARMUSH M L,GOLBERG A,SER?A G,et al.Electroporation-based technologies for medicine:principles,applications,and challenges[J].Annual Review of Biomedical Engineering,2014,16(16):295-320.
[8]赵亚军,姚陈果,董守龙,等.基于遗传算法的不可逆电穿孔肿瘤消融治疗计划的优化[J].高电压技术,2017,43(2):627-636.ZHAO Yajun,YAO Chenguo,DONG Shoulong,et al.Optimization of the treatment planning for the tumor ablation of irreversible electroporation based on genetic algorithm[J].High Voltage Engineering,2017,43(2):627-636.
[9]姚陈果,赵亚军,李成祥,等.不可逆电穿孔微创消融肿瘤技术的研究进展[J].高电压技术,2014,40(12):3725-3737.YAO Chenguo,ZHAO Yajun,LI Chengxiang,et al.Recent advances in tissue minimally invasive ablation with irreversible electroporation[J].High Voltage Engineering,2014,40(12):3725-3737.
[10]李成祥,姚陈果,米彦,等.物理消融肿瘤的研究进展[J].生物医学工程学杂志,2009(5):1137-1140.LI Chengxiang,YAO Chenguo,MI Yan,et al.Recent advances on physical ablation for tumor[J].Journal of Biomedical Engineering,2009(5):1137-1140.
[11]LENCIONI R,CROCETTI L.Radio frequency ablation of liver cancer[J].Techniques in Vascular and Interventional Radiology,2007,10(1):38-46.
[12]于哲,吴家昌,张明华,等.微波消融治疗骨肉瘤诱导免疫原性细胞死亡的机制研究[J].中华放射肿瘤学杂志,2016,25(6):602-608.YU Zhe,WU Jiachang,ZHANG Minghua,et al.Mechanism of immunogenic cell death induced by microwave ablation treatment of osteosarcoma[J].Chinese Journal of Radiation Oncology,2016,25(6):602-608.
[13]何志祝,郑永昌,刘静.脑肿瘤冷冻消融区域影响因素分析[J].工程热物理学报,2016,37(8):1753-1757.HE Zhizhu,ZHENG Yongchang,LIU Jing.Analyzing the influence factors of brain tumor cryoablation[J].Journal of Engineering Thermophysics,2016,37(8):1753-1757.
[14]YAO C G,SUN C X,MI Y,et al.Experimental studies on killing and inhibiting effects of steep pulsed electric field(SPEF)to target cancer cell and solid tumor[J].IEEE Transactions on Plasma Science,2004,32(4):1626-1633.
[15]TANG L L,YAO C G,SUN C X.Apoptosis induction with electric pulses—a new approach to cancer therapy with drug free[J].Biochemical and Biophysical Research Communications,2009,390(4):1098-1101.
[16]YAO C G,HU X,MI Y,et al.Window effect of pulsed electric field on biological cells[J].IEEE Transactions on Dielectrics and Electrical Insulation,2009,16(5):1259-1266.
[17]LIU Y,XIONG Z A,ZHOU W,et al.Percutaneous ultrasound-guided irreversible electroporation:a goat liver study[J].Oncology Letters,2012,4(3):450-454.
[18]DAVALOS R V,MIR L M,RUBINSKY B.Tissue ablation with irreversible electroporation[J].Annals of Biomedical Engineering,2005,33(2):223-231.
[19]MILLER L,LEOR J,RUBINSKY B.Cancer cells ablation with irreversible electroporation[J].Technology in Cancer Research&Treatment,2005,4(6):699-705.
[20]GARCIA P A,ROSSMEISL J H,II R E N,et al.Intracranial nonthermal irreversible electroporation:in vivo analysis[J].Journal of Membrane Biology,2010,236(1):127-136.
[21]GARCIA P A,DAVALOS R V,MIKLAVCIC D.A numerical investigation of the electric and thermal cell kill distributions in electroporation-based therapies in tissue[J].Plos One,2014,9(8):e103083.
[22]NEAL R E,GARCIA P A,ROSSMEISL J H,et al.A study using irreversible electroporation to treat large,irregular tumors in a canine patient[C]∥International Conference of the IEEE Engineering in Medicine&Biology Society.[S.l.]:IEEE,2010:2747.
[23]ONIK G,RUBINSKY B.Irreversible electroporation:first patient experience focal therapy of prostate cancer[M].Berlin,Germany:Springer,2010:235-247.
[24]GUO Y,ZHANG Y,KLEIN R,et al.Irreversible electroporation therapy in the liver:longitudinal efficacy studies in a rat model of hepatocellular carcinoma[J].Cancer Research,2010,70(4):1555-1563.
[25]QIN Z,JIANG J,LONG G,et al.Irreversible electroporation:an in vivo study with dorsal skin fold chamber[J].Annals of Biomedical Engineering,2013,41(3):619-629.
[26]PHILIPS P,LI Y,LI S,et al.Efficacy of irreversible electroporation in human pancreatic adenocarcinoma:advanced murine model[J].Molecular Therapy—Methods&Clinical Development,2015,2:15001.
[27]THOMSON K R,CHEUNG W,ELLIS S J,et al.Investigation of the safety of irreversible electroporation in humans[J].Journal of Vascular&Interventional Radiology Jvir,2011,22(5):611-621.
[28]KINGHAM T P,KARKAR A M,D'ANGELICA M I,et al.Ablation of perivascular hepatic malignant tumors with irreversible electroporation[J].Journal of the American College of Surgeons,2012,215(3):379-387.
[29]万军平.肝动脉介入热化疗治疗转移性肝肿瘤的临床应用[J].临床医学工程,2012,19(11):1917-1918.WANG Junping.Clinical application of metastatic liver treated by hepatic artery involved thermo-chemotherapy[J].Medicine Healthcare Apparatus,2012,19(11):1917-1918.
[30]谢晓燕,吕明德,殷晓煜,等.超声引导经皮射频消融治疗肝癌的研究[J].中华外科杂志,2003,41(1):23-26.XIE Xiaoyan,LüMingde,YIN Xiaoyu,et al.Application of ultrasound-guided percutaneous radiofrequency ablation in treatment of liver cancer[J].Chinese Journal of Surgery,2003,41(1):23-26.
[31]CHEUNG W,KAVNOUDIAS H,ROBERTS S,et al.Irreversible electroporation for unresectable hepatocellular carcinoma:initial experience and review of safety and outcomes[J].Technology in Cancer Research&Treatment,2013,12(3):233-241.
[32]GOLBERG A,YARMUSH M L.Nonthermal irreversible electroporation:fundamentals,applications,and challenges[J].IEEE Transactions on Biomedical Engineering,2013,60(3):707-714.
[33]MARTIN R C,SCHWARTZ E,ADAMS J,et al.Intra-operative anesthesia management in patients undergoing surgical irreversible electroporation of the pancreas,liver,kidney,and retroperitoneal tumors[J].Anesthesiology and Pain Medicine,2015,5(3):e22786.
[34]GOLBERG A,BRUINSMA B G,UYGUN B E,et al.Tissue heterogeneity in structure and conductivity contribute to cell survival during irreversible electroporation ablation by“electric field sinks”[J].Scientific Reports,2015,5:8485.
[35]董守龙.高频双极性微秒脉冲电场不可逆电穿孔消融肿瘤的实验与机理研究[D].重庆:重庆大学,2017.DONG Shoulong.Experiments and mechanism research of irreversible electroporation by high frequency bipolar microsecond pulse for tumor ablation[D].Chongqing,China:Chongqing University,2017.
[36]ARENA C B,SANO M B,RYLANDER M N,et al.Theoretical considerations of tissue electroporation with high-frequency bipolar pulses[J].IEEE Transactions on Biomedical Engineering,2011,58(5):1474-1482.
[37]MIKLAV?I?D,PUCIHAR G,PAVLOVEC M,et al.The effect of high frequency electric pulses on muscle contractions and antitumor efficiency in vivo for a potential use in clinical electrochemotherapy[J].Bioelectrochemistry,2005,65:121-128.
[38]MI Y,RUI S Q,LI C X,et al.Multi-parametric study of temperature and thermal damage of tumor exposed to high-frequency nanosecond-pulsed electric fields based on finite element simulation[J].Medical&Biological Engineering&Computing,2017,55(7):1109-1122.
[39]ARENA C B,SANO M B,ROSSMEISL J H,et al.High-frequency irreversible electroporation(H-FIRE)for non-thermal ablation without muscle contraction[J].Biomedical Engineering Online,2011,10(1):102.
[40]SWEENEY D C,REBER?EK M,DERMOL J,et al.Quantification of cell membrane permeability induced by monopolar and high-frequency bipolar bursts of electrical pulses[J].Biochimica et Biophysica Acta(BBA)-Biomembranes,2016,1858(11):2689-2698.
[41]IVEY J W,LATOUCHE E L,SANO M B,et al.Targeted cellular ablation based on the morphology of malignant cells[J].Scientific Reports,2015,5:17157.
[42]YAO C G,LIU H M,ZHAO Y J,et al.Analysis of dynamic processes in single-cell electroporation and their effects on parameter selection based on the finite-element model[J].IEEE Transactions on Plasma Science,2017,45(5):889-900.
[43]YAO C,ZHAO Y,DONG S,et al.Differences in the effects of duty cycle and interval on cell response induced by high-frequency pulses under different pulse durations[J].IEEE Transactions on Plasma Science,2016,44(10):2097-2110.
[44]SANO M B,ARENA C B,DEWITT M R,et al.In-vitro bipolar nano-and microsecond electro-pulse bursts for irreversible electroporation therapies[J].Bioelectrochemistry,2014,100:69-79.
[45]YAO C G,ZHAO Y J,MI Y,et al.Comparative study of the biological responses to conventional pulse and high-frequency monopolar pulse bursts[J].IEEE Transactions on Plasma Science,2017,45(10):2629-2638.
[46]YAO C G,ZHAO Y J,LIU H M,et al.Dielectric variations of potato induced by irreversible electroporation under different pulses based on the cole-cole model[J].IEEE Transactions on Dielectrics and Electrical Insulation,2017,24(4):2225-2233.
[47]BHONSLE S P,ARENA C B,SWEENEY D C,et al.Mitigation of impedance changes due to electroporation therapy using bursts of high-frequency bipolar pulses[J].Biomedical Engineering Online,2015,14(3):1-14.
[48]SANO M B,ARENA C B,BITTLEMAN K R,et al.Bursts of bipolar microsecond pulses inhibit tumor growth[J].Scientific Reports,2015,5:14999.
[49]ARENA C B,GARCIA P A,SANO M B,et al.Focal blood-brainbarrier disruption with high-frequency pulsed electric fields[J].Technology,2014,2(3):206-213.
[50]YAO C G,DONG S L,ZHAO Y J,et al.Bipolar microsecond pulses and insulated needle electrodes for reducing muscle contractions during irreversible electroporation[J].IEEE Transactions on Biomedical Engineering,2017,64(12):2924-2937.
[51]姚陈果,赵亚军,董守龙,等.基于阻抗谱的脉冲电场诱导兔肝脏组织不可逆电穿孔程度评估新方法的实验研究[J].高电压技术,2017,43(8):2454-2463.YAO Chenguo,ZHAO Yajun,DONG Shoulong,et al.Study on the impedance characteristic of rabbit liver induced by irreversible electroporation caused by high-frequency and conventional pulsed electric field[J].High Voltage Engineering,2017,43(8):2454-2463.
[52]SIDDIQUI I A,LATOUCHE E L,DEWITT M R,et al.Induction of rapid,reproducible hepatic ablations using next-generation,high frequency irreversible electroporation(H-FIRE)in vivo[J].HPB,2016,18(9):726-734.